TGD5_ARATH
ID TGD5_ARATH Reviewed; 91 AA.
AC Q8VY77;
DT 09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein TRIGALACTOSYLDIACYLGLYCEROL 5, chloroplastic {ECO:0000303|PubMed:26410300};
GN Name=TGD5 {ECO:0000303|PubMed:26410300};
GN OrderedLocusNames=At1g27695 {ECO:0000312|Araport:AT1G27695};
GN ORFNames=T22C5 {ECO:0000312|EMBL:AC012375};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAL62371.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-41, DISRUPTION
RP PHENOTYPE, AND INTERACTION WITH TGD1; TGD2; TGD3 AND TGD4.
RX PubMed=26410300; DOI=10.1105/tpc.15.00394;
RA Fan J., Zhai Z., Yan C., Xu C.;
RT "Arabidopsis TRIGALACTOSYLDIACYLGLYCEROL5 interacts with TGD1, TGD2, and
RT TGD4 to facilitate lipid transfer from the endoplasmic reticulum to
RT plastids.";
RL Plant Cell 27:2941-2955(2015).
CC -!- FUNCTION: Facilitates lipid transfer from the outer to the inner
CC plastid envelope by bridging TGD4 with the TGD1,2,3 transport complex.
CC Required for endoplasmic reticulum-to-plastid lipid trafficking as part
CC of the eukaryotic pathway of thylakoid lipid assembly.
CC {ECO:0000269|PubMed:26410300}.
CC -!- SUBUNIT: Interacts with TGD1, TGD2, TGD3 and TGD4.
CC {ECO:0000269|PubMed:26410300}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:26410300}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Is resistant to both thermolysin and trypsin like
CC an inner envelope-localized protein, but physically interacts with the
CC outer envelope protein TGD4. {ECO:0000269|PubMed:26410300}.
CC -!- DISRUPTION PHENOTYPE: Growth retardation, reduced rosette size,
CC slightly pale color and early flowering. Deficiency in eukaryotic
CC thylakoid galactolipids and accumulation of trigalactosyldiacylglycerol
CC (TGDG) and phosphatidylcholine (PC). Changed fatty acid profile in
CC leaves with a substential increase in 16:0, 18:1 and 18:2 at the
CC expense of 16:3 and 18:3. {ECO:0000269|PubMed:26410300}.
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DR EMBL; AC012375; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE30864.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30865.1; -; Genomic_DNA.
DR EMBL; AY072379; AAL62371.1; -; mRNA.
DR EMBL; BT024637; ABD57462.1; -; mRNA.
DR RefSeq; NP_001031097.1; NM_001036020.2.
DR RefSeq; NP_683330.1; NM_148489.3.
DR AlphaFoldDB; Q8VY77; -.
DR STRING; 3702.AT1G27695.1; -.
DR TCDB; 8.A.86.1.1; the chloroplast trigalactosyldiacylglycerol-5 (tgd5) family.
DR PaxDb; Q8VY77; -.
DR PRIDE; Q8VY77; -.
DR ProteomicsDB; 246412; -.
DR EnsemblPlants; AT1G27695.1; AT1G27695.1; AT1G27695.
DR EnsemblPlants; AT1G27695.2; AT1G27695.2; AT1G27695.
DR GeneID; 839662; -.
DR Gramene; AT1G27695.1; AT1G27695.1; AT1G27695.
DR Gramene; AT1G27695.2; AT1G27695.2; AT1G27695.
DR KEGG; ath:AT1G27695; -.
DR Araport; AT1G27695; -.
DR TAIR; locus:504956157; AT1G27695.
DR eggNOG; ENOG502S2VR; Eukaryota.
DR HOGENOM; CLU_095133_1_0_1; -.
DR OMA; NFNGAGV; -.
DR OrthoDB; 1649115at2759; -.
DR PRO; PR:Q8VY77; -.
DR Proteomes; UP000006548; Chromosome 1.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0020037; F:heme binding; IBA:GO_Central.
DR GO; GO:0005319; F:lipid transporter activity; IGI:TAIR.
DR GO; GO:1901965; P:endoplasmic reticulum to chloroplast transport; IGI:TAIR.
DR GO; GO:1990052; P:ER to chloroplast lipid transport; IGI:TAIR.
DR GO; GO:0010441; P:guard cell development; IMP:TAIR.
DR GO; GO:0009667; P:plastid inner membrane organization; IMP:TAIR.
DR GO; GO:0009666; P:plastid outer membrane organization; IMP:TAIR.
PE 1: Evidence at protein level;
KW Chloroplast; Lipid transport; Membrane; Plastid; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..91
FT /note="Protein TRIGALACTOSYLDIACYLGLYCEROL 5,
FT chloroplastic"
FT /id="PRO_0000434890"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 41
FT /note="G->D: In tgd5-1; loss of function."
FT /evidence="ECO:0000269|PubMed:26410300"
SQ SEQUENCE 91 AA; 9174 MW; 90C1CA4EA4921CCF CRC64;
MVLSDFTGVG VGFGFGVGCG FGVGWGFGGM PMNILGVGAG GGCGVGLGLG WGFGTAFGSH
YRSSRLTFQG IELETADKRE EVVANMSKNS T
