ID   TGDS_BOVIN              Reviewed;         355 AA.
AC   A6QLW2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=dTDP-D-glucose 4,6-dehydratase;
DE            EC=4.2.1.46;
GN   Name=TGDS;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal pons;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC         + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC         ChEBI:CHEBI:57649; EC=4.2.1.46;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. dTDP-glucose dehydratase subfamily. {ECO:0000305}.
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DR   EMBL; BC148106; AAI48107.1; -; mRNA.
DR   RefSeq; NP_001094629.1; NM_001101159.1.
DR   AlphaFoldDB; A6QLW2; -.
DR   SMR; A6QLW2; -.
DR   STRING; 9913.ENSBTAP00000006984; -.
DR   PaxDb; A6QLW2; -.
DR   PRIDE; A6QLW2; -.
DR   GeneID; 534594; -.
DR   KEGG; bta:534594; -.
DR   CTD; 23483; -.
DR   eggNOG; KOG0747; Eukaryota.
DR   HOGENOM; CLU_007383_1_14_1; -.
DR   InParanoid; A6QLW2; -.
DR   OrthoDB; 815803at2759; -.
DR   TreeFam; TF313892; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IBA:GO_Central.
DR   GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR   CDD; cd05246; dTDP_GD_SDR_e; 1.
DR   InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   2: Evidence at transcript level;
KW   Lyase; NAD; Reference proteome.
FT   CHAIN           1..355
FT                   /note="dTDP-D-glucose 4,6-dehydratase"
FT                   /id="PRO_0000328213"
FT   ACT_SITE        143
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        144
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        166
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         142
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   355 AA;  40666 MW;  871C965B58BEF68C CRC64;
     MSVAGRAESL GPPDSFAKRV LVTGGAGFIA SHMIVSLVED YPNYMIINLD KLDYCASLKN
     LETISNKQNY KFIQGDICDS HFVKLLFETE KIDIVLHFAA QTHVDLSFVR AFEFTYVNVY
     GTHVLVSAAH EARVEKFIYV STDEVYGGSL DKEFDESSPK QPTNPYASSK AAAECFVQSY
     WEQYKFPVVI TRSSNVYGPH QYPEKVIPKF ISLLQHNRKC CIHGTGLQTR NFLYATDVVE
     AFLTVLKKGK PGEIYNIGTN FEMSVLQLAK ELIQLIKETN SESEMENWVD YVDDRPTNDM
     RYPMKSEKIH GLGWRPKVPW KEGIKKTIEW YRENFHNWKN AEKALEPFPV QPPFV