TGDS_MIMIV
ID TGDS_MIMIV Reviewed; 323 AA.
AC Q5UR12;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Putative dTDP-D-glucose 4,6-dehydratase;
DE EC=4.2.1.46;
GN OrderedLocusNames=MIMI_R141;
OS Acanthamoeba polyphaga mimivirus (APMV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC Imitervirales; Mimiviridae; Mimivirus.
OX NCBI_TaxID=212035;
OH NCBI_TaxID=5757; Acanthamoeba polyphaga (Amoeba).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Rowbotham-Bradford;
RX PubMed=15486256; DOI=10.1126/science.1101485;
RA Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H.,
RA La Scola B., Susan M., Claverie J.-M.;
RT "The 1.2-megabase genome sequence of Mimivirus.";
RL Science 306:1344-1350(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC ChEBI:CHEBI:57649; EC=4.2.1.46;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. dTDP-glucose dehydratase subfamily. {ECO:0000305}.
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DR EMBL; AY653733; AAV50416.1; -; Genomic_DNA.
DR RefSeq; YP_003986633.1; NC_014649.1.
DR PDB; 6VLO; X-ray; 2.05 A; A/B/C/D=1-323.
DR PDBsum; 6VLO; -.
DR SMR; Q5UR12; -.
DR GeneID; 9924741; -.
DR KEGG; vg:9924741; -.
DR Proteomes; UP000001134; Genome.
DR GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR CDD; cd05246; dTDP_GD_SDR_e; 1.
DR InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Lyase; NAD; Reference proteome.
FT CHAIN 1..323
FT /note="Putative dTDP-D-glucose 4,6-dehydratase"
FT /id="PRO_0000309205"
FT ACT_SITE 125
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 126
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 149
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 124
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT STRAND 2..7
FT /evidence="ECO:0007829|PDB:6VLO"
FT TURN 8..10
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 12..24
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 29..34
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 42..44
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 51..55
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 61..70
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 75..78
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 91..99
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 101..113
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 117..124
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:6VLO"
FT TURN 134..136
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 139..143
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 148..167
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 171..176
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 178..181
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 189..198
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 203..206
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 212..217
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 218..231
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 237..240
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 245..247
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 248..259
FT /evidence="ECO:0007829|PDB:6VLO"
FT TURN 264..266
FT /evidence="ECO:0007829|PDB:6VLO"
FT STRAND 267..270
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 285..289
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 299..313
FT /evidence="ECO:0007829|PDB:6VLO"
FT HELIX 314..316
FT /evidence="ECO:0007829|PDB:6VLO"
SQ SEQUENCE 323 AA; 36874 MW; A5289D45C9EF8D4B CRC64;
MKNILVTGGL GFIGSNFVNH ISSKYDNVNI YVYDIGDYCA SVENVEWNNR TKLIKGDIRN
FDLIMHTLTE HEIDTIVHFA AHSHVDNSFK NSLAFTETNV FGTHVLLECS RMYGKLKLFF
HMSTDEVYGE IDTTDTSREV SLLCPTNPYA ATKAGAEHIV KSYFLSYKLP IIIARCNNVY
GRNQYPEKLI PKFICSLLDG KKLHIQGTGN SRRNFIHAID VADAVDLVIN NGVIGETYNI
GVTNEHSVLD VAQILCDIAG VNLENQLEYV PDRLFNDFRY NITNDKIKSL GWEQSRKDFK
KELVELFDWY KVNRHRYNIP GSQ
