TGDS_MOUSE
ID TGDS_MOUSE Reviewed; 355 AA.
AC Q8VDR7; Q3U4A6;
DT 23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=dTDP-D-glucose 4,6-dehydratase;
DE EC=4.2.1.46;
GN Name=Tgds;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC ChEBI:CHEBI:57649; EC=4.2.1.46;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. dTDP-glucose dehydratase subfamily. {ECO:0000305}.
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DR EMBL; AK154341; BAE32527.1; -; mRNA.
DR EMBL; BC021419; AAH21419.1; -; mRNA.
DR CCDS; CCDS27332.1; -.
DR RefSeq; NP_083854.3; NM_029578.3.
DR AlphaFoldDB; Q8VDR7; -.
DR SMR; Q8VDR7; -.
DR STRING; 10090.ENSMUSP00000022727; -.
DR iPTMnet; Q8VDR7; -.
DR PhosphoSitePlus; Q8VDR7; -.
DR PaxDb; Q8VDR7; -.
DR PRIDE; Q8VDR7; -.
DR Antibodypedia; 24784; 103 antibodies from 19 providers.
DR DNASU; 76355; -.
DR Ensembl; ENSMUST00000022727; ENSMUSP00000022727; ENSMUSG00000022130.
DR GeneID; 76355; -.
DR KEGG; mmu:76355; -.
DR UCSC; uc007uyo.2; mouse.
DR CTD; 23483; -.
DR MGI; MGI:1923605; Tgds.
DR VEuPathDB; HostDB:ENSMUSG00000022130; -.
DR eggNOG; KOG0747; Eukaryota.
DR GeneTree; ENSGT00940000159196; -.
DR HOGENOM; CLU_007383_1_14_1; -.
DR InParanoid; Q8VDR7; -.
DR OMA; KLIPLMC; -.
DR OrthoDB; 815803at2759; -.
DR PhylomeDB; Q8VDR7; -.
DR TreeFam; TF313892; -.
DR BioGRID-ORCS; 76355; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q8VDR7; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8VDR7; protein.
DR Bgee; ENSMUSG00000022130; Expressed in animal zygote and 255 other tissues.
DR ExpressionAtlas; Q8VDR7; baseline and differential.
DR Genevisible; Q8VDR7; MM.
DR GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IBA:GO_Central.
DR GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR CDD; cd05246; dTDP_GD_SDR_e; 1.
DR InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Lyase; NAD; Reference proteome.
FT CHAIN 1..355
FT /note="dTDP-D-glucose 4,6-dehydratase"
FT /id="PRO_0000183251"
FT ACT_SITE 143
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 144
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 166
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 142
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 205
FT /note="K -> R (in Ref. 2; AAH21419)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 355 AA; 40570 MW; FE0018334153E5DD CRC64;
MSAASREERS GPPGSFAKRV LVTGGAGFIA SHVIVSLVED YPDYMIVNLD KLDYCASLKN
LEPVSNKQNY KFIQGDICDS HFVKLLFEVE KIDIVLHFAA QTHVDLSFVR AFEFTYVNVY
GTHVLVNAAY EAGVEKFIYV STDEVYGGSL DQEFDESSPK QPTNPYASSK AAAECFVQSY
WERYKFPVVI TRSSNVYGPH QYPEKVIPKF ISLLQHNRKC CIHGSGLQRR NFLYAADVVE
AFLTVLTKGE PGEIYNIGTN FEMSVVQLAK ELIQLIKETN SESETESWVD YVSDRPHNDM
RYPMKSEKIH SLGWKPKVPW EEGIKKTVEW YRKNFHNWKN AEKALEPFPV QPPFM
