TGIF1_MOUSE
ID TGIF1_MOUSE Reviewed; 272 AA.
AC P70284; Q921F9; Q99JS3;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 17-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Homeobox protein TGIF1;
DE AltName: Full=5'-TG-3'-interacting factor 1;
GN Name=Tgif1; Synonyms=Tgif;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=BALB/cJ; TISSUE=Liver;
RX PubMed=8901052;
RX DOI=10.1002/(sici)1097-0177(199604)205:4<410::aid-aja5>3.0.co;2-l;
RA Bertolino E., Wildt S., Richards G., Clerc R.G.;
RT "Expression of a novel murine homeobox gene in the developing cerebellar
RT external granular layer during its proliferation.";
RL Dev. Dyn. 205:410-420(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Mammary fibroblast, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Binds to a retinoid X receptor (RXR) responsive element from
CC the cellular retinol-binding protein II promoter (CRBPII-RXRE).
CC Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription
CC activation of the retinoic acid responsive element. May participate in
CC the transmission of nuclear signals during development and in the
CC adult, as illustrated by the down-modulation of the RXR alpha
CC activities (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with SMAD2 (By similarity). Interacts with CTBP,
CC SMAD3 and HDAC1. {ECO:0000250, ECO:0000250|UniProtKB:Q15583}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P70284-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P70284-2; Sequence=VSP_002297;
CC -!- SIMILARITY: Belongs to the TALE/TGIF homeobox family. {ECO:0000305}.
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DR EMBL; X89749; CAA61896.1; -; mRNA.
DR EMBL; BC005724; AAH05724.1; -; mRNA.
DR EMBL; BC012700; AAH12700.1; -; mRNA.
DR CCDS; CCDS37683.1; -. [P70284-1]
DR CCDS; CCDS50174.1; -. [P70284-2]
DR RefSeq; NP_001157546.1; NM_001164074.1. [P70284-2]
DR RefSeq; NP_001157548.1; NM_001164076.1. [P70284-2]
DR RefSeq; NP_001157549.1; NM_001164077.1. [P70284-2]
DR RefSeq; NP_033398.2; NM_009372.3. [P70284-1]
DR RefSeq; XP_006524095.1; XM_006524032.2. [P70284-2]
DR AlphaFoldDB; P70284; -.
DR BMRB; P70284; -.
DR SMR; P70284; -.
DR BioGRID; 204166; 6.
DR STRING; 10090.ENSMUSP00000130930; -.
DR iPTMnet; P70284; -.
DR PhosphoSitePlus; P70284; -.
DR MaxQB; P70284; -.
DR PaxDb; P70284; -.
DR PRIDE; P70284; -.
DR ProteomicsDB; 262898; -. [P70284-1]
DR ProteomicsDB; 262899; -. [P70284-2]
DR Antibodypedia; 3890; 396 antibodies from 33 providers.
DR DNASU; 21815; -.
DR Ensembl; ENSMUST00000059775; ENSMUSP00000060512; ENSMUSG00000047407. [P70284-1]
DR Ensembl; ENSMUST00000118283; ENSMUSP00000113192; ENSMUSG00000047407. [P70284-2]
DR Ensembl; ENSMUST00000172229; ENSMUSP00000127139; ENSMUSG00000047407. [P70284-2]
DR GeneID; 21815; -.
DR KEGG; mmu:21815; -.
DR UCSC; uc008dlp.2; mouse. [P70284-1]
DR CTD; 7050; -.
DR MGI; MGI:1194497; Tgif1.
DR VEuPathDB; HostDB:ENSMUSG00000047407; -.
DR eggNOG; KOG0773; Eukaryota.
DR GeneTree; ENSGT00940000155230; -.
DR HOGENOM; CLU_034318_2_0_1; -.
DR InParanoid; P70284; -.
DR OMA; FPLCQSV; -.
DR PhylomeDB; P70284; -.
DR Reactome; R-MMU-2173795; Downregulation of SMAD2/3:SMAD4 transcriptional activity.
DR BioGRID-ORCS; 21815; 21 hits in 78 CRISPR screens.
DR ChiTaRS; Tgif1; mouse.
DR PRO; PR:P70284; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; P70284; protein.
DR Bgee; ENSMUSG00000047407; Expressed in embryonic post-anal tail and 222 other tissues.
DR ExpressionAtlas; P70284; baseline and differential.
DR Genevisible; P70284; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0070410; F:co-SMAD binding; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0008432; F:JUN kinase binding; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0035881; P:amacrine cell differentiation; IDA:MGI.
DR GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:MGI.
DR GO; GO:0048144; P:fibroblast proliferation; IMP:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:MGI.
DR GO; GO:0048387; P:negative regulation of retinoic acid receptor signaling pathway; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0001843; P:neural tube closure; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IDA:MGI.
DR GO; GO:0038092; P:nodal signaling pathway; IGI:MGI.
DR GO; GO:1902871; P:positive regulation of amacrine cell differentiation; IDA:MGI.
DR GO; GO:0048146; P:positive regulation of fibroblast proliferation; IMP:MGI.
DR GO; GO:0010470; P:regulation of gastrulation; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060041; P:retina development in camera-type eye; IDA:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR Pfam; PF05920; Homeobox_KN; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..272
FT /note="Homeobox protein TGIF1"
FT /id="PRO_0000049320"
FT DNA_BIND 35..97
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 24..28
FT /note="CTBP-binding motif"
FT VAR_SEQ 1..20
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_002297"
FT CONFLICT 35
FT /note="K -> M (in Ref. 1; CAA61896)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 272 AA; 29573 MW; 625528713B4C0C42 CRC64;
MKSKKGLVAA SGSDSEDEDS MDSPLDLSSS AASGKRRRRG NLPKESVQIL RDWLYEHRYN
AYPSEQEKAL LSQQTHLSTL QVCNWFINAR RRLLPDMLRK DGKDPNQFTI SRRGAKISEA
SSIEAAMGIK NFMPTLEESP FHSCVVGPNP TLGRPVSPKP PSPGSILARP SVICHTTVTA
LKDGPFSLCQ PIGVGQSTDV PQIAPSNFTD TSLVYPEDTC KSGPSPNPQS GLFNTPPPTP
PDLNQDFSGF QLLVDVALKR AAEMELQAKL TA
