TGL3_SCHPO
ID TGL3_SCHPO Reviewed; 545 AA.
AC Q9Y7P3;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2012, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Triacylglycerol lipase ptl1;
DE EC=3.1.1.3 {ECO:0000250|UniProtKB:P40308};
GN Name=ptl1; ORFNames=SPCC1450.16c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP FUNCTION.
RX PubMed=22592553; DOI=10.1007/s00253-012-4151-8;
RA Yazawa H., Kumagai H., Uemura H.;
RT "Characterization of triglyceride lipase genes of fission yeast
RT Schizosaccharomyces pombe.";
RL Appl. Microbiol. Biotechnol. 96:981-991(2012).
CC -!- FUNCTION: Lipid particle-localized triacylglycerol (TAG) lipase. The
CC lipid droplet/particle is a lipid storage compartment which serves as a
CC depot of energy and building blocks for membrane lipid biosynthesis.
CC Involved in the mobilization of the non-polar storage lipids
CC triacylglycerols (TAGs) from lipid particles by hydrolysis of TAGs,
CC releasing and supplying specific fatty acids to the appropriate
CC metabolic pathways. {ECO:0000269|PubMed:22592553}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid +
CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
CC Evidence={ECO:0000250|UniProtKB:P40308};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12045;
CC Evidence={ECO:0000250|UniProtKB:P40308};
CC -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:P40308}.
CC Note=Partially retained in the endoplasmic reticulum in cells lacking
CC triacylglycerols. {ECO:0000250|UniProtKB:P40308}.
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DR EMBL; CU329672; CAB40183.2; -; Genomic_DNA.
DR PIR; T40998; T40998.
DR RefSeq; NP_588315.2; NM_001023305.2.
DR AlphaFoldDB; Q9Y7P3; -.
DR BioGRID; 275901; 13.
DR STRING; 4896.SPCC1450.16c.1; -.
DR MaxQB; Q9Y7P3; -.
DR PaxDb; Q9Y7P3; -.
DR PRIDE; Q9Y7P3; -.
DR EnsemblFungi; SPCC1450.16c.1; SPCC1450.16c.1:pep; SPCC1450.16c.
DR GeneID; 2539335; -.
DR KEGG; spo:SPCC1450.16c; -.
DR PomBase; SPCC1450.16c; ptl1.
DR VEuPathDB; FungiDB:SPCC1450.16c; -.
DR eggNOG; KOG2214; Eukaryota.
DR HOGENOM; CLU_009031_5_1_1; -.
DR InParanoid; Q9Y7P3; -.
DR OMA; LAEFWTV; -.
DR PRO; PR:Q9Y7P3; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005811; C:lipid droplet; IEA:UniProtKB-SubCell.
DR GO; GO:0004806; F:triglyceride lipase activity; IMP:PomBase.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006642; P:triglyceride mobilization; IMP:PomBase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR002641; PNPLA_dom.
DR InterPro; IPR021771; Triacylglycerol_lipase_N.
DR Pfam; PF11815; DUF3336; 1.
DR Pfam; PF01734; Patatin; 1.
DR SUPFAM; SSF52151; SSF52151; 1.
DR PROSITE; PS51635; PNPLA; 1.
PE 3: Inferred from homology;
KW Hydrolase; Lipid degradation; Lipid droplet; Lipid metabolism;
KW Reference proteome.
FT CHAIN 1..545
FT /note="Triacylglycerol lipase ptl1"
FT /id="PRO_0000317234"
FT DOMAIN 182..358
FT /note="PNPLA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT MOTIF 213..217
FT /note="GXSXG"
SQ SEQUENCE 545 AA; 62546 MW; 4769AD9F56852B10 CRC64;
MTALFNFWRI MYANISSIWL LVVSFFEWLF SATSISQQRG SGPRKGKVVM NKDCRSWEDW
KVLATTIDKA SGRWKWRFTP ASDKYDYLLI DRCTVSLKRY RQRKSVYPML MFLRSSLLRN
FGNIGNSSLY TENYSGTKIL IEEYVREVNN CLEFLYHTKR LSYDVKCDFF SAARISFGTT
CLYFNGGTAF GLYHFGVAKT LWKRNLLPQI LAGCASGALI ASLLSVYRDE ELNGLFDTFP
SELWKICQQT SDYSLSKVVE YGNMLDISMI ASFVRQRLGT ITFQEAFERT GRIVNIVAPP
SAVSGSPQVL NYFTAPNVLI WSAVCSSNSW AAIYRSSPLL AKLPDGSTEV CTPKNFIWPY
AGLPNTGRSN PYARISEIFN VNHFVITQSR PSLFPTFYDE LHHHRVSGYS LKMIRLVGLE
MAYRFRQLDI LGLLPPRLRR FFVDDYVPSA YITLTPTFSF SDIKHAFTKP SLSDIQYWIL
VGERATWQAI PLLQVRCKTE ISLRHLSKNL TNSYVEPLSV NNLASPFVTN LEENQEKMLK
IFKVK
