TGON3_RAT
ID TGON3_RAT Reviewed; 357 AA.
AC P19814; Q4G0B6;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Trans-Golgi network integral membrane protein TGN38;
DE Flags: Precursor;
GN Name=Ttgn1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=2204342; DOI=10.1042/bj2700097;
RA Luzio J.P., Brake B., Banting G., Howell K.E., Braghetta P., Stanley K.K.;
RT "Identification, sequencing and expression of an integral membrane protein
RT of the trans-Golgi network (TGN38).";
RL Biochem. J. 270:97-102(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 119-125, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Hippocampus;
RA Lubec G., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [4]
RP INTERACTION WITH NEURABIN-1 AND NEURABIN-2, AND MUTAGENESIS.
RX PubMed=10514494; DOI=10.1074/jbc.274.42.30080;
RA Stephens D.J., Banting G.;
RT "Direct interaction of the trans-Golgi network membrane protein, TGN38,
RT with the F-actin binding protein, neurabin.";
RL J. Biol. Chem. 274:30080-30086(1999).
RN [5]
RP METHYLATION AT ARG-74, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=15047867; DOI=10.1091/mbc.e04-02-0101;
RA Wu C.C., MacCoss M.J., Mardones G., Finnigan C., Mogelsvang S.,
RA Yates J.R. III, Howell K.E.;
RT "Organellar proteomics reveals Golgi arginine dimethylation.";
RL Mol. Biol. Cell 15:2907-2919(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234; SER-235 AND SER-271, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- SUBUNIT: Interacts with neurabin-1 and neurabin-2. Binds preferentially
CC to the dimeric form of neurabin-1. {ECO:0000269|PubMed:10514494}.
CC -!- INTERACTION:
CC P19814; P84092: Ap2m1; NbExp=4; IntAct=EBI-541446, EBI-297693;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
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DR EMBL; X53565; CAA37637.1; -; mRNA.
DR EMBL; BC098512; AAH98512.1; -; mRNA.
DR RefSeq; NP_620195.1; NM_138840.2.
DR PDB; 4IKN; X-ray; 1.85 A; B=348-353.
DR PDBsum; 4IKN; -.
DR AlphaFoldDB; P19814; -.
DR SMR; P19814; -.
DR BioGRID; 251332; 2.
DR ELM; P19814; -.
DR IntAct; P19814; 7.
DR MINT; P19814; -.
DR STRING; 10116.ENSRNOP00000019713; -.
DR GlyGen; P19814; 4 sites.
DR iPTMnet; P19814; -.
DR PhosphoSitePlus; P19814; -.
DR PaxDb; P19814; -.
DR PRIDE; P19814; -.
DR Ensembl; ENSRNOT00000019713; ENSRNOP00000019713; ENSRNOG00000014617.
DR GeneID; 192152; -.
DR KEGG; rno:192152; -.
DR UCSC; RGD:620445; rat.
DR CTD; 10618; -.
DR RGD; 620445; Ttgn1.
DR eggNOG; ENOG502S6YU; Eukaryota.
DR GeneTree; ENSGT00530000064712; -.
DR HOGENOM; CLU_047350_0_0_1; -.
DR InParanoid; P19814; -.
DR OMA; MWRSRRI; -.
DR OrthoDB; 1324458at2759; -.
DR PhylomeDB; P19814; -.
DR Reactome; R-RNO-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-RNO-432722; Golgi Associated Vesicle Biogenesis.
DR Reactome; R-RNO-6811440; Retrograde transport at the Trans-Golgi-Network.
DR Reactome; R-RNO-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-RNO-8856828; Clathrin-mediated endocytosis.
DR Reactome; R-RNO-8957275; Post-translational protein phosphorylation.
DR PRO; PR:P19814; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000014617; Expressed in colon and 19 other tissues.
DR Genevisible; P19814; RN.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005768; C:endosome; ISO:RGD.
DR GO; GO:0005794; C:Golgi apparatus; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; TAS:RGD.
DR GO; GO:0005802; C:trans-Golgi network; IDA:MGI.
DR GO; GO:0030140; C:trans-Golgi network transport vesicle; IBA:GO_Central.
DR GO; GO:0006895; P:Golgi to endosome transport; TAS:RGD.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Glycoprotein; Golgi apparatus;
KW Membrane; Methylation; Phosphoprotein; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..357
FT /note="Trans-Golgi network integral membrane protein TGN38"
FT /id="PRO_0000022488"
FT TOPO_DOM 18..303
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 325..357
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 133..140
FT /note="1"
FT REPEAT 141..148
FT /note="2"
FT REPEAT 149..156
FT /note="3"
FT REPEAT 157..164
FT /note="4"
FT REPEAT 165..172
FT /note="5"
FT REPEAT 173..180
FT /note="6"
FT REGION 20..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 133..180
FT /note="6 X 8 AA tandem repeats of [PT]-[TS]-G-[GVS]-D-[SN]-
FT [DN]-[NK]"
FT COMPBIAS 20..34
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..71
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..194
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..240
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 241..259
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 279..295
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 74
FT /note="Dimethylated arginine"
FT /evidence="ECO:0000269|PubMed:15047867"
FT MOD_RES 234
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 235
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 271
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 26
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 140
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 147
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 348
FT /note="S->A,D: Abolishes neurabin-1 and neurabin-2
FT binding."
FT /evidence="ECO:0000269|PubMed:10514494"
FT MUTAGEN 350
FT /note="Y->A: No effect on neurabin-1 and neurabin-2
FT binding."
FT /evidence="ECO:0000269|PubMed:10514494"
FT MUTAGEN 354..357
FT /note="Missing: No effect on neurabin-1 and neurabin-2
FT binding."
FT /evidence="ECO:0000269|PubMed:10514494"
SQ SEQUENCE 357 AA; 38305 MW; 173B64C51CAD593B CRC64;
MQFLVALLLL SVAVARALPS ASKPNNTSSE NNPPIQPSTP LPPGVDISQQ VKTNRPTDQR
LESDKEGQDK TVARTSASVS SGVESATNLN LDDSKKHPET ADAKLKETLQ QLLPVDPKQE
KSGQKFTKDS GSPTGGDSDN TTGGDSNKTT GVDSDKTSGG DSNKPTGSDN DKPTGGDSNK
PTSKVPSNTE TPKIDKVQLT EKGQKPTLIS KTESGEKLAG DSDFSLKPEK GDKSSEPTED
VETKEIEEGD TEPEEGSPLE EENEKVLGPS SSENQEGTLT DSMKDEKDDH YKDNSGNTSA
ESSHFFAYLV TAAVLVAVLY IAYHNKRKII AFALEGKRSK VTRRPKASDY QRLNLKL
