ID   TGP1_TETTH              Reviewed;         725 AA.
AC   O43952;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=G-quartet DNA-binding protein TGP1;
GN   Name=TGP1;
OS   Tetrahymena thermophila.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=5911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 368-390.
RC   STRAIN=C3-V;
RX   PubMed=9512530; DOI=10.1093/nar/26.7.1613;
RA   Lu Q., Schierer T.P., Kang S.-G., Henderson E.;
RT   "Purification, characterization and molecular cloning of TGP1, a novel G-
RT   DNA binding protein from Tetrahymena thermophila.";
RL   Nucleic Acids Res. 26:1613-1620(1998).
RN   [2]
RP   CHARACTERIZATION.
RC   STRAIN=C3-V;
RX   PubMed=7509637; DOI=10.1021/bi00174a034;
RA   Schierer T., Henderson E.;
RT   "A protein from Tetrahymena thermophila that specifically binds parallel-
RT   stranded G4-DNA.";
RL   Biochemistry 33:2240-2246(1994).
CC   -!- FUNCTION: Binds specifically to parallel G4-DNA, a four-stranded
CC       structure stabilized by tetrads of hydrogen-bonded guanines.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- PTM: The N-terminus is blocked.
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DR   EMBL; AF006380; AAC48333.1; -; mRNA.
DR   PIR; T03219; T03219.
DR   AlphaFoldDB; O43952; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 2.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   SUPFAM; SSF50249; SSF50249; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Nucleus.
FT   CHAIN           1..725
FT                   /note="G-quartet DNA-binding protein TGP1"
FT                   /id="PRO_0000072510"
FT   REGION          252..284
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          468..614
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           241..258
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        260..275
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        468..482
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        484..498
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        499..513
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        514..551
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        562..609
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        385
FT                   /note="K -> R (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   725 AA;  83212 MW;  7175AC9FA9C85205 CRC64;
     MTDQGVAKEQ YITVGSLKEI ADPVKKFLRK GNRKGRNQKE LKFRAKVVNQ QLADETGSIN
     FTTEGVAVNN NQTYEFTGSL KVVDYKLNLA VTSATPAKDQ IANLGSEQLS KADIDQHSGK
     HIVLIKDLIN LRIGQSFFAR PKDIVHNNEQ RRMTATLADN TAAIQAEISV NKKSITQEVL
     ESLNSDKVYL FSDVVLNFNE SNKLVARFNY RSNIVEANDR QIQQLSNNNL SAKEYSDETE
     KKALTDVLQI HEKKERVHKQ QNKNKNPRNA HKNHNRQRPN LQETEPVKIS GLQQWNNNQT
     VIGKIVSLRT EDKSLPAKQD GTSTTMVYLK GTIGDETGVI DFDMAEKRDC PRFKENDVVK
     FTSVMNKGRQ SAEGKVGGHY IEVKKFGQYI ILSDHNINNV NLNNNLSNLE LTARPKNPNP
     RFIKGEFVGV KEEEKDGNIQ YTYTVRSKEG EEQSITIRNK ITTLKVGEIH KIDRERKRSS
     SRPNHQGGQR GNRSHSQNNR NQRNRDKHHN SQNNQPNKYK NTSVQNNNNN KNQQRSQSQN
     QRPPRNYDNR QGGENRNNRQ RNENNRNNFN GNGHRVNNQN NQRNRNSSYP RNNNYDHHHN
     QQTDISGLEP GKRGQNVTGQ VIEVSEFSKQ INDKTLHFVK GRIADENANI RFDIKKPQNL
     EIKVGEVYNF KDVNNKVDDN GYHYIDLNRF GRVFPSHKKF QSINDKRNCD ADRSSIEYVK
     KTVPN