ID   TGS1_MYCTO              Reviewed;         463 AA.
AC   P9WKC8; E0YJK4; F2GJ38; O07035; P0A650;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Probable diacyglycerol O-acyltransferase tgs1;
DE            Short=TGS1;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Probable triacylglycerol synthase tgs1;
GN   Name=tgs1; OrderedLocusNames=MT3216;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, AND DORMANCY REGULON.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12953092; DOI=10.1084/jem.20030205;
RA   Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA   Sherman D.R., Schoolnik G.K.;
RT   "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT   tuberculosis dormancy program.";
RL   J. Exp. Med. 198:705-713(2003).
CC   -!- FUNCTION: Catalyzes the terminal and only committed step in
CC       triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as
CC       substrates. Required for storage lipid synthesis.
CC       {ECO:0000250|UniProtKB:P9WKC9}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- INDUCTION: A member of the dormancy regulon. Induced in response to
CC       reduced oxygen tension (hypoxia) and low levels of nitric oxide (NO).
CC       {ECO:0000269|PubMed:12953092}.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK47554.1; -; Genomic_DNA.
DR   PIR; H70922; H70922.
DR   RefSeq; WP_003917740.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKC8; -.
DR   SMR; P9WKC8; -.
DR   EnsemblBacteria; AAK47554; AAK47554; MT3216.
DR   KEGG; mtc:MT3216; -.
DR   PATRIC; fig|83331.31.peg.3466; -.
DR   HOGENOM; CLU_024186_3_1_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..463
FT                   /note="Probable diacyglycerol O-acyltransferase tgs1"
FT                   /id="PRO_0000427676"
FT   ACT_SITE        137
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   463 AA;  50755 MW;  2F0E680C2BE957CF CRC64;
     MNHLTTLDAG FLKAEDVDRH VSLAIGALAV IEGPAPDQEA FLSSLAQRLR PCTRFGQRLR
     LRPFDLGAPK WVDDPDFDLG RHVWRIALPR PGNEDQLFEL IADLMARRLD RGRPLWEVWV
     IEGLADSKWA ILTKLHHCMA DGIAATHLLA GLSDESMSDS FASNIHTTMQ SQSASVRRGG
     FRVNPSEALT ASTAVMAGIV RAAKGASEIA AGVLSPAASS LNGPISDLRR YSAAKVPLAD
     VEQVCRKFDV TINDVALAAI TESYRNVFIQ RGERPRFDSL RTLVPVSTRS NSALSKTDNR
     VSLMLPNLPV DQENPLQRLR IVHSRLTRAK AGGQRQFGNT LMAIANRLPF PMTAWAVGLL
     MRLPQRGVVT VATNVPGPRR PLQIMGRRVL DLYPVSPIAM QLRTSVAMLS YADDLYFGIL
     ADYDVVADAG QLARGIEDAV ARLVAISKRR KVTRRRGALS LVV