ID   TGS4_MYCTO              Reviewed;         474 AA.
AC   P9WKC2; L0TBT5; O53305; P67208;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Probable diacyglycerol O-acyltransferase Tgs4;
DE            Short=TGS4;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC3};
DE   AltName: Full=Probable triacylglycerol synthase tgs4;
GN   Name=tgs4; OrderedLocusNames=MT3173;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Required for maintaining the appropriate mycolic acid
CC       composition and permeability of the envelope on its exposure to acidic
CC       pH. {ECO:0000250|UniProtKB:P9WKC3}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC3};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK47509.1; -; Genomic_DNA.
DR   PIR; D70853; D70853.
DR   RefSeq; WP_003416079.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKC2; -.
DR   SMR; P9WKC2; -.
DR   EnsemblBacteria; AAK47509; AAK47509; MT3173.
DR   KEGG; mtc:MT3173; -.
DR   PATRIC; fig|83331.31.peg.3419; -.
DR   HOGENOM; CLU_024186_4_1_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..474
FT                   /note="Probable diacyglycerol O-acyltransferase Tgs4"
FT                   /id="PRO_0000427679"
FT   ACT_SITE        135
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   474 AA;  50887 MW;  36832D972BE3851A CRC64;
     MTRINPIDLS FLLLERANRP NHMAAYTIFE KPKGQKSSFG PRLFDAYRHS QAAKPFNHKL
     KWLGTDVAAW ETVEPDMGYH IRHLALPAPG SMQQFHETVS FLNTGLLDRG HPMWECYIID
     GIERGRIAIL LKVHHALIDG EGGLRAMRNF LSDSPDDTTL AGPWMSAQGA DRPRRTPATV
     SRRAQLQGQL QGMIKGLTKL PSGLFGVSAD AADLGAQALS LKARKASLPF TARRTLFNNT
     AKSAARAYGN VELPLADVKA LAKATGTSVN DVVMTVIDDA LHHYLAEHQA STDRPLVAFM
     PMSLREKSGE GGGNRVSAEL VPMGAPKASP VERLKEINAA TTRAKDKGRG MQTTSRQAYA
     LLLLGSLTVA DALPLLGKLP SANVVISNMK GPTEQLYLAG APLVAFSGLP IVPPGAGLNV
     TFASINTALC IAIGAAPEAV HEPSRLAELM QRAFTELQTE AGTTSPTTSK SRTP