BRE4_YEAST
ID BRE4_YEAST Reviewed; 1125 AA.
AC Q07660; D6VRC5;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Protein BRE4;
DE AltName: Full=Brefeldin A-sensitivity protein 4;
GN Name=BRE4; OrderedLocusNames=YDL231C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=11169758;
RX DOI=10.1002/1097-0061(20010130)18:2<163::aid-yea659>3.0.co;2-#;
RA Muren E., Oeyen M., Barmark G., Ronne H.;
RT "Identification of yeast deletion strains that are hypersensitive to
RT brefeldin A or monensin, two drugs that affect intracellular transport.";
RL Yeast 18:163-172(2001).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: May be involved in intracellular vesicle transport.
CC {ECO:0000269|PubMed:11169758}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Deletion of the gene leads to brefeldin A-sensitivity.
CC -!- SIMILARITY: Belongs to the BRE4 family. {ECO:0000305}.
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DR EMBL; Z74279; CAA98810.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11635.1; -; Genomic_DNA.
DR PIR; S67794; S67794.
DR RefSeq; NP_010050.1; NM_001180291.1.
DR AlphaFoldDB; Q07660; -.
DR SMR; Q07660; -.
DR BioGRID; 31880; 35.
DR DIP; DIP-5168N; -.
DR IntAct; Q07660; 1.
DR STRING; 4932.YDL231C; -.
DR iPTMnet; Q07660; -.
DR MaxQB; Q07660; -.
DR PaxDb; Q07660; -.
DR PRIDE; Q07660; -.
DR EnsemblFungi; YDL231C_mRNA; YDL231C; YDL231C.
DR GeneID; 851367; -.
DR KEGG; sce:YDL231C; -.
DR SGD; S000002390; BRE4.
DR VEuPathDB; FungiDB:YDL231C; -.
DR eggNOG; KOG4711; Eukaryota.
DR HOGENOM; CLU_004486_0_0_1; -.
DR InParanoid; Q07660; -.
DR OMA; FWGWAAN; -.
DR BioCyc; YEAST:G3O-29610-MON; -.
DR PRO; PR:Q07660; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q07660; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006897; P:endocytosis; IMP:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR018820; BRE4-related_DUF2421.
DR InterPro; IPR023244; Brefeldin_A-sensitivity_4.
DR Pfam; PF10334; ArAE_2; 1.
DR PRINTS; PR02047; BREFELDNASP4.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1125
FT /note="Protein BRE4"
FT /id="PRO_0000239634"
FT TOPO_DOM 1..143
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..176
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..198
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 220..227
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..284
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..706
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 707..727
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 728..750
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 751..771
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 772..780
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 781..801
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 802..843
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 844..864
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 865..1125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 426..449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 427..446
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 830
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1125 AA; 129725 MW; 3E0E04F6916A7E74 CRC64;
MKRYERDRSP TPDPDIVKGS YSQTSLRSLH ELNYKNPAGI SGLSFAGSPQ QSVASLSQMR
LENLVKDKHW EEVEDFGLEE LRDGFFDAAF TKPDSKARSP NSDIDDDNGA ARKKLQSGFT
KLSEYVWTAI YRPIIHFPRD IRKNGVSIFK FFIAYFIAIV ICVIRPSGRW IGHEFRYFLP
IAVLIHHPVR NIGVQLEMTI SSIIGASFGL GWSALAWYIS TATKPTANYQ GGILFQSLTM
ALLFAIWLRS VYRRFFYFTT SFSISIIFTH TVRLASSKFD LKWQIFWDFG ISYLFGLLLS
LLVCVCVSPH SGNAELMEHY NKCLQTTKTF LMALVDTELI KSKEQIYLAQ VKMVKTLNID
LSQGFRDFVN QLTISRFDLQ SLKSLRNSLT AMETSLRVLP IAPKIFNDDE LKKMYEELEK
YRSDSATLSK EASASPQSSG IPTRENTPSA FKPIGPGLLK NEIYINALKA SFSKSIFNLI
LEMIFVLENL SRVLKKYESP NQKNNLDECV KILSHSHSKL KRKIYKLDVC YRDFVNSSFF
SQELLNDEES VDIFLFLRYL RNSARQLVTV IHDCQVLGEN IHWRIALPSY PLSRALTRLP
KQCVLDEGAG NVLHYFEAKR DVDEIFERVY NTYTSRHKYN KGEEEALRLD SQGGDEKSQN
RKNHTISIRA IDHNDFNFHT TQNPWRFKLW KLSRILSGDE CKWTLKITFC MIFLCLPTWL
PESYHWYQEF HCWWAPLTFY LLAHRRYSGN WALVMRRLIC GIVGIFWGWA ANQSRHFGSP
YVVCTFAGLI VVPFSINFLV YRNTKSSFTA LMCFTIIALE PYSKPNRHYN LTTAGIWKST
WVTGLALIIG ILVSIPINWI VWPFRARTEL RDSMSSLLAH LGQSYQTVAD RYLYRDADDA
PTDLTFAFSH IREVRLTQSL EAIRELLKKA RHEPIIISNF NPEKYASLIN SCQFLLSKII
EARISGAFFE IWDQDFDIET TRALLSLRRD SVSSVIFVFY ILSNCFRSKN KIPRYLPNPI
MSRKKLYHFI KKFSEMKDQS HSNLNSGGNS MEKNLFKKIY QQKASSSGQQ QLPLPSVANS
SEIDSEKMHW TEVHGIAFAR AFTDISEALF QVESCAKDIL GEENF
