BRG1_CANAL
ID BRG1_CANAL Reviewed; 422 AA.
AC Q59LY1; A0A1D8PDH2;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Biofilm regulator 1;
GN Name=BRG1; Synonyms=GAT2; OrderedLocusNames=CAALFM_C105140WA;
GN ORFNames=CaO19.11538, CaO19.4056;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION.
RX PubMed=12773383; DOI=10.1093/emboj/cdg256;
RA Uhl M.A., Biery M., Craig N., Johnson A.D.;
RT "Haploinsufficiency-based large-scale forward genetic analysis of
RT filamentous growth in the diploid human fungal pathogen C.albicans.";
RL EMBO J. 22:2668-2678(2003).
RN [5]
RP INDUCTION.
RX PubMed=18321992; DOI=10.1091/mbc.e07-09-0960;
RA Goyard S., Knechtle P., Chauvel M., Mallet A., Prevost M.C., Proux C.,
RA Coppee J.Y., Schwartz P., Dromer F., Park H., Filler S.G., Janbon G.,
RA d'Enfert C.;
RT "The Yak1 kinase is involved in the initiation and maintenance of hyphal
RT growth in Candida albicans.";
RL Mol. Biol. Cell 19:2251-2266(2008).
RN [6]
RP FUNCTION.
RX PubMed=20041210; DOI=10.1371/journal.pgen.1000783;
RA Homann O.R., Dea J., Noble S.M., Johnson A.D.;
RT "A phenotypic profile of the Candida albicans regulatory network.";
RL PLoS Genet. 5:E1000783-E1000783(2009).
RN [7]
RP INDUCTION.
RX PubMed=21414038; DOI=10.1111/j.1365-2958.2011.07626.x;
RA Bonhomme J., Chauvel M., Goyard S., Roux P., Rossignol T., d'Enfert C.;
RT "Contribution of the glycolytic flux and hypoxia adaptation to efficient
RT biofilm formation by Candida albicans.";
RL Mol. Microbiol. 80:995-1013(2011).
RN [8]
RP FUNCTION.
RX PubMed=22757963; DOI=10.1111/j.1365-2958.2012.08127.x;
RA Cleary I.A., Lazzell A.L., Monteagudo C., Thomas D.P., Saville S.P.;
RT "BRG1 and NRG1 form a novel feedback circuit regulating Candida albicans
RT hypha formation and virulence.";
RL Mol. Microbiol. 85:557-573(2012).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22276126; DOI=10.1371/journal.pone.0029707;
RA Du H., Guan G., Xie J., Sun Y., Tong Y., Zhang L., Huang G.;
RT "Roles of Candida albicans Gat2, a GATA-type zinc finger transcription
RT factor, in biofilm formation, filamentous growth and virulence.";
RL PLoS ONE 7:E29707-E29707(2012).
RN [10]
RP FUNCTION.
RX PubMed=23049891; DOI=10.1371/journal.pone.0045912;
RA Chauvel M., Nesseir A., Cabral V., Znaidi S., Goyard S.,
RA Bachellier-Bassi S., Firon A., Legrand M., Diogo D., Naulleau C.,
RA Rossignol T., d'Enfert C.;
RT "A versatile overexpression strategy in the pathogenic yeast Candida
RT albicans: identification of regulators of morphogenesis and fitness.";
RL PLoS ONE 7:E45912-E45912(2012).
RN [11]
RP FUNCTION, INTERACTION WITH HDA1, SUBCELLULAR LOCATION, AND DNA-BINDING.
RX PubMed=22536157; DOI=10.1371/journal.ppat.1002663;
RA Lu Y., Su C., Liu H.;
RT "A GATA transcription factor recruits Hda1 in response to reduced Tor1
RT signaling to establish a hyphal chromatin state in Candida albicans.";
RL PLoS Pathog. 8:E1002663-E1002663(2012).
RN [12]
RP FUNCTION, AND INDUCTION.
RX PubMed=23171549; DOI=10.1091/mbc.e12-06-0477;
RA Su C., Lu Y., Liu H.;
RT "Reduced TOR signaling sustains hyphal development in Candida albicans by
RT lowering Hog1 basal activity.";
RL Mol. Biol. Cell 24:385-397(2013).
RN [13]
RP FUNCTION IN WHITE-OPAQUE SWITCHING.
RX PubMed=23555196; DOI=10.1371/journal.pbio.1001525;
RA Xie J., Tao L., Nobile C.J., Tong Y., Guan G., Sun Y., Cao C.,
RA Hernday A.D., Johnson A.D., Zhang L., Bai F.Y., Huang G.;
RT "White-opaque switching in natural MTLa/alpha isolates of Candida albicans:
RT evolutionary implications for roles in host adaptation, pathogenesis, and
RT sex.";
RL PLoS Biol. 11:E1001525-E1001525(2013).
RN [14]
RP FUNCTION.
RX PubMed=23637598; DOI=10.1371/journal.ppat.1003305;
RA Lin C.H., Kabrawala S., Fox E.P., Nobile C.J., Johnson A.D., Bennett R.J.;
RT "Genetic control of conventional and pheromone-stimulated biofilm formation
RT in Candida albicans.";
RL PLoS Pathog. 9:E1003305-E1003305(2013).
CC -!- FUNCTION: Transcription factor required for hyphal growth, biofilm
CC formation, and virulence. Promotes formation of both conventional and
CC pheromone-stimulated biofilms. Binds and recruits HDA1 to promoters of
CC hypha-specific genes in a rapamycin-dependent manner. Involved in the
CC switch between two heritable states, the white and opaque states. These
CC two cell types differ in many characteristics, including cell
CC structure, mating competence, and virulence. Each state is heritable
CC for many generations, and switching between states occurs
CC stochastically at low frequency. {ECO:0000269|PubMed:12773383,
CC ECO:0000269|PubMed:20041210, ECO:0000269|PubMed:22276126,
CC ECO:0000269|PubMed:22536157, ECO:0000269|PubMed:22757963,
CC ECO:0000269|PubMed:23049891, ECO:0000269|PubMed:23171549,
CC ECO:0000269|PubMed:23555196, ECO:0000269|PubMed:23637598}.
CC -!- SUBUNIT: Interacts with HDA1. {ECO:0000269|PubMed:22536157}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22536157}.
CC -!- INDUCTION: Up-regulation during the yeast-to-hypha transition is
CC dependent upon the function of YAK1. HOG1 represses the expression of
CC BRG1 via the transcriptional repressor SKO1. Regulated by TYE7 during
CC late-stage biofilm formation. {ECO:0000269|PubMed:18321992,
CC ECO:0000269|PubMed:21414038, ECO:0000269|PubMed:23171549}.
CC -!- DISRUPTION PHENOTYPE: Attenuates virulence in a mouse model of systemic
CC infection. {ECO:0000269|PubMed:22276126}.
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DR EMBL; CP017623; AOW26184.1; -; Genomic_DNA.
DR RefSeq; XP_710725.2; XM_705633.2.
DR AlphaFoldDB; Q59LY1; -.
DR SMR; Q59LY1; -.
DR STRING; 237561.Q59LY1; -.
DR PRIDE; Q59LY1; -.
DR GeneID; 3647664; -.
DR KEGG; cal:CAALFM_C105140WA; -.
DR CGD; CAL0000196982; BRG1.
DR VEuPathDB; FungiDB:C1_05140W_A; -.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_731585_0_0_1; -.
DR InParanoid; Q59LY1; -.
DR OrthoDB; 1538591at2759; -.
DR PRO; PR:Q59LY1; -.
DR Proteomes; UP000000559; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00320; GATA; 1.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 1: Evidence at protein level;
KW Cell adhesion; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Virulence; Zinc; Zinc-finger.
FT CHAIN 1..422
FT /note="Biofilm regulator 1"
FT /id="PRO_0000422799"
FT ZN_FING 282..307
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 1..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 116..207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 422 AA; 45513 MW; 41BA1B04FEBC4F7A CRC64;
MSSSSSLSSS TTTATTTSAR IRLPSISELT SRSTISGGSN NGNGSALKSQ ISPRLSDTSR
ILPSILKNTS GSSTPTSSST PFKCPPIKST VGGTLSSGNT QSNYVLGNTK INSLPRLSSP
TLPVKVQPQQ QPQLPPASSL SPVTRVINTP PQQPQSVSAS TSPNTQYQYY QYQQQSSPIQ
QQQQQQQATP AATPTVMQMA QNQPSHPAPL QYATQQYYPQ PVYYQSPAGV PPPPPSVTHQ
GHIIAVHQHP GHLPQVGVNG MPPNVGYTIV QPEIVNKSTN RCHRCGTTET PEWRRGPKGV
RTLCNACGLF HAKLVKRKGA ALAAEEVLNN KVTKGKNGRR ISMKKHLLNE SLKQQQQING
VGIPINGFNH QILPPSFKPQ QGGIATLPPL MHGQYPNNVN NLVIHQPPPQ QQQQQQQHNN
IC
