BRH1_ARATH
ID BRH1_ARATH Reviewed; 170 AA.
AC Q9XF92; A0A178VCF4;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Brassinosteroid-responsive RING protein 1 {ECO:0000303|PubMed:12012249};
GN Name=BRH1 {ECO:0000303|PubMed:12012249};
GN OrderedLocusNames=At3g61460 {ECO:0000312|Araport:AT3G61460};
GN ORFNames=F2A19.60 {ECO:0000312|EMBL:CAB71076.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION
RP BY BRASSINOSTEROIDS.
RC STRAIN=cv. Columbia;
RX PubMed=12012249; DOI=10.1007/s00425-001-0723-z;
RA Molnar G., Bancos S., Nagy F., Szekeres M.;
RT "Characterisation of BRH1, a brassinosteroid-responsive RING-H2 gene from
RT Arabidopsis thaliana.";
RL Planta 215:127-133(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=11983057; DOI=10.1186/gb-2002-3-4-research0016;
RA Kosarev P., Mayer K.F.X., Hardtke C.S.;
RT "Evaluation and classification of RING-finger domains encoded by the
RT Arabidopsis genome.";
RL Genome Biol. 3:RESEARCH0016.1-RESEARCH0016.12(2002).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY BRASSINOSTEROIDS, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=28887625; DOI=10.1007/s11427-017-9133-8;
RA Wang X., Chen E., Ge X., Gong Q., Butt H., Zhang C., Yang Z., Li F.,
RA Zhang X.;
RT "Overexpressed BRH1, a RING finger gene, alters rosette leaf shape in
RT Arabidopsis thaliana.";
RL Sci. China Life Sci. 61:79-87(2018).
CC -!- FUNCTION: May be involved in the brassinosteroids (BRs) signaling
CC pathway and regulate the growth and development of rosette leaves
CC (PubMed:28887625). Seems to prevent over development of leaves and
CC inflorescence stems (PubMed:12012249). {ECO:0000269|PubMed:12012249,
CC ECO:0000269|PubMed:28887625}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in stems, rosette leaves and
CC siliques, and moderately expressed in roots, cauline leaves and flower
CC (PubMed:28887625). Detected at low levels in seeds (PubMed:28887625).
CC {ECO:0000269|PubMed:28887625}.
CC -!- INDUCTION: Repressed by brassinosteroids (BRs) such as brassinolide and
CC 24-epi-brassinolide in a BRI1-dependent manner (PubMed:12012249).
CC Induced rapidly but transiently induced by chitin, a fungal pathogen
CC elicitor (PubMed:12012249). {ECO:0000269|PubMed:12012249}.
CC -!- DISRUPTION PHENOTYPE: Vigorous plants with thick inflorescence stems
CC and enlarged leaves; more rounded tip in rosette leaves and more
CC serrated cauline leaves. {ECO:0000269|PubMed:12012249,
CC ECO:0000269|PubMed:28887625}.
CC -!- SIMILARITY: Belongs to the RING-type zinc finger family. {ECO:0000305}.
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DR EMBL; AF134155; AAD27870.1; -; mRNA.
DR EMBL; AL132962; CAB71076.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE80208.1; -; Genomic_DNA.
DR EMBL; AY065300; AAL38776.1; -; mRNA.
DR EMBL; AY117307; AAM51382.1; -; mRNA.
DR EMBL; AY086562; AAM63625.1; -; mRNA.
DR PIR; T47938; T47938.
DR RefSeq; NP_191705.1; NM_116011.2.
DR AlphaFoldDB; Q9XF92; -.
DR SMR; Q9XF92; -.
DR IntAct; Q9XF92; 7.
DR STRING; 3702.AT3G61460.1; -.
DR PaxDb; Q9XF92; -.
DR PRIDE; Q9XF92; -.
DR EnsemblPlants; AT3G61460.1; AT3G61460.1; AT3G61460.
DR GeneID; 825319; -.
DR Gramene; AT3G61460.1; AT3G61460.1; AT3G61460.
DR KEGG; ath:AT3G61460; -.
DR Araport; AT3G61460; -.
DR TAIR; locus:2082757; AT3G61460.
DR eggNOG; KOG0800; Eukaryota.
DR HOGENOM; CLU_013137_18_2_1; -.
DR InParanoid; Q9XF92; -.
DR OMA; MANCKHM; -.
DR OrthoDB; 1353630at2759; -.
DR PhylomeDB; Q9XF92; -.
DR PRO; PR:Q9XF92; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9XF92; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:1901371; P:regulation of leaf morphogenesis; IMP:UniProtKB.
DR GO; GO:1905421; P:regulation of plant organ morphogenesis; IMP:UniProtKB.
DR GO; GO:0009741; P:response to brassinosteroid; IEP:UniProtKB.
DR GO; GO:0010200; P:response to chitin; IEP:TAIR.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR011016; Znf_RING-CH.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF13639; zf-RING_2; 1.
DR SMART; SM00184; RING; 1.
DR SMART; SM00744; RINGv; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 2: Evidence at transcript level;
KW Brassinosteroid signaling pathway; Developmental protein; Membrane;
KW Metal-binding; Reference proteome; Transmembrane; Transmembrane helix;
KW Zinc; Zinc-finger.
FT CHAIN 1..170
FT /note="Brassinosteroid-responsive RING protein 1"
FT /id="PRO_0000449047"
FT TRANSMEM 15..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ZN_FING 94..137
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
SQ SEQUENCE 170 AA; 20060 MW; F25DAAD643C42761 CRC64;
MGFPVGYTEV FLPKLFVQTL SILGFIRTIV FSIFRFLGLS DFLEMDQTWP DYTSYPTRIP
ETRSPFSALL IREILPVIKF EELTNSGEDL PENCAVCLYE FEGEQEIRWL RNCRHIFHRS
CLDRWMDHDQ KTCPLCRTPF VPDEMQEEFN QRLWAASGVH DFHCPVTELL
