ID   BRHP_CONIM              Reviewed;           7 AA.
AC   P58803;
DT   02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2002, sequence version 1.
DT   22-APR-2020, entry version 47.
DE   RecName: Full=Bromoheptapeptide Im;
OS   Conus imperialis (Imperial cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Stephanoconus.
OX   NCBI_TaxID=35631;
RN   [1]
RP   PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, PYROGLUTAMATE FORMATION AT GLN-1,
RP   BROMINATION AT TRP-6, AMIDATION AT CYS-7, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=9030520; DOI=10.1074/jbc.272.8.4689;
RA   Craig A.G., Jimenez E.C., Dykert J., Nielsen D.B., Gulyas J.,
RA   Abogadie F.C., Porter J., Rivier J.E., Cruz L.J., Olivera B.M.,
RA   McIntosh J.M.;
RT   "A novel post-translational modification involving bromination of
RT   tryptophan. Identification of the residue, L-6-bromotryptophan, in peptides
RT   from Conus imperialis and Conus radiatus venom.";
RL   J. Biol. Chem. 272:4689-4698(1997).
CC   -!- FUNCTION: Does not elicit gross behavioral symptoms when injected
CC       centrally or peripherally in mice. {ECO:0000269|PubMed:9030520}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- DOMAIN: The cysteine framework is C-C.
CC   -!- MASS SPECTROMETRY: Mass=853.19; Method=LSI;
CC       Evidence={ECO:0000269|PubMed:9030520};
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DR   PIR; A58512; A58512.
DR   ConoServer; 3552; Bromoheptapeptide Im.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Bromination; Direct protein sequencing; Disulfide bond;
KW   Pyrrolidone carboxylic acid; Secreted; Toxin.
FT   PEPTIDE         1..7
FT                   /note="Bromoheptapeptide Im"
FT                   /id="PRO_0000044512"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   MOD_RES         6
FT                   /note="6'-bromotryptophan"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   MOD_RES         7
FT                   /note="Cysteine amide"
FT                   /evidence="ECO:0000269|PubMed:9030520"
FT   DISULFID        2..7
SQ   SEQUENCE   7 AA;  795 MW;  6EA37DC6D87EA6B0 CRC64;
     QCGQAWC