BRI3_HUMAN
ID BRI3_HUMAN Reviewed; 125 AA.
AC O95415; D6W5R8; F5GXW6; Q8WV52; Q9UIC6;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Brain protein I3;
DE AltName: Full=pRGR2;
GN Name=BRI3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RA Adler H.J., Warner S.J., Rossi A.M., Lomax M.I.;
RT "The gene encoding the chick orthologue of mouse brain protein I3 is
RT expressed in the inner ear.";
RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Gastric adenocarcinoma;
RA Kato S.;
RT "Human full-length cDNA for I3 protein.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Cervix carcinoma;
RA Hu Y.F., Mao X.H., Zhuang M., Lu C.D.;
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP INTERACTION WITH BRI3BP.
RX PubMed=11860200; DOI=10.1023/a:1013807518791;
RA Lin L., Wu Y., Li C., Zhao S.;
RT "Cloning, tissue expression pattern, and chromosome location of a novel
RT human gene BRI3BP.";
RL Biochem. Genet. 39:369-377(2001).
RN [8]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=14592447; DOI=10.1016/j.bbrc.2003.10.038;
RA Wu H., Liu G., Li C., Zhao S.;
RT "bri3, a novel gene, participates in tumor necrosis factor-alpha-induced
RT cell death.";
RL Biochem. Biophys. Res. Commun. 311:518-524(2003).
RN [9]
RP FUNCTION, AND INDUCTION.
RX PubMed=20538055; DOI=10.1016/j.cellsig.2010.05.021;
RA Kavak E., Najafov A., Ozturk N., Seker T., Cavusoglu K., Aslan T.,
RA Duru A.D., Saygili T., Hoxhaj G., Hiz M.C., Unal D.O., Birguel-Iyison N.,
RA Ozturk M., Koman A.;
RT "Analysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide
RT microarray and promoter analysis: Identification of BRI3 and HSF2 as novel
RT targets.";
RL Cell. Signal. 22:1523-1535(2010).
RN [10]
RP INTERACTION WITH BRI3BP; MGAT1 AND IFITM3 (ISOFORMS 1 AND 2), AND
RP SUBCELLULAR LOCATION (ISOFORMS 1 AND 2).
RX PubMed=30983867; DOI=10.3906/biy-1805-47;
RA Akiva I., Birguel Iyison N.;
RT "Identification of IFITM3 and MGAT1 as novel interaction partners of BRI3
RT by yeast two-hybrid screening.";
RL Turk. J. Biol. 42:463-470(2018).
CC -!- FUNCTION: Participates in tumor necrosis factor-alpha (TNF)-induced
CC cell death (PubMed:14592447). May be a target of Wnt/beta-catenin
CC signaling in the liver (PubMed:20538055). {ECO:0000269|PubMed:14592447,
CC ECO:0000269|PubMed:20538055}.
CC -!- SUBUNIT: [Isoform 1]: Interacts with BRI3BP (PubMed:11860200,
CC PubMed:30983867). Interacts with MGAT1 and IFITM3 (PubMed:30983867).
CC {ECO:0000269|PubMed:11860200, ECO:0000269|PubMed:30983867}.
CC -!- SUBUNIT: [Isoform 2]: Interacts with BRI3BP, MGAT1 and IFITM3; the
CC interactions are weaker than with isoform 1.
CC {ECO:0000269|PubMed:30983867}.
CC -!- INTERACTION:
CC O95415; O95870: ABHD16A; NbExp=3; IntAct=EBI-2874789, EBI-348517;
CC O95415; Q8WY22: BRI3BP; NbExp=3; IntAct=EBI-2874789, EBI-359348;
CC O95415; Q01628: IFITM3; NbExp=6; IntAct=EBI-2874789, EBI-7932862;
CC O95415; Q13021: MALL; NbExp=3; IntAct=EBI-2874789, EBI-750078;
CC O95415; P26572: MGAT1; NbExp=6; IntAct=EBI-2874789, EBI-7211952;
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000305|PubMed:14592447};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:30983867}. Note=Co-localizes with MGAT1 and IFITM3
CC at the perinuclear region. {ECO:0000269|PubMed:30983867}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm
CC {ECO:0000269|PubMed:30983867}. Nucleus {ECO:0000269|PubMed:30983867}.
CC Note=Diffuse localization in the cytoplasm and nucleus.
CC {ECO:0000269|PubMed:30983867}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000312|HGNC:HGNC:1109}; Synonyms=a
CC {ECO:0000303|PubMed:30983867};
CC IsoId=O95415-1; Sequence=Displayed;
CC Name=2 {ECO:0000312|HGNC:HGNC:1109}; Synonyms=b
CC {ECO:0000303|PubMed:30983867};
CC IsoId=O95415-2; Sequence=VSP_060620, VSP_060621;
CC -!- INDUCTION: Up-regulated during TNF-mediated inflammation and immunity
CC (By similarity). Up-regulated by beta-catenin and TCF4
CC (PubMed:20538055). {ECO:0000250|UniProtKB:P28662,
CC ECO:0000269|PubMed:20538055}.
CC -!- SIMILARITY: Belongs to the BRI3 family. {ECO:0000305}.
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DR EMBL; AF106966; AAD05167.1; -; mRNA.
DR EMBL; AB055977; BAB32785.1; -; mRNA.
DR EMBL; AF041430; AAF18565.2; -; mRNA.
DR EMBL; AC004841; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC091654; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC093169; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471091; EAW76713.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76714.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76715.1; -; Genomic_DNA.
DR EMBL; BC018737; AAH18737.1; -; mRNA.
DR EMBL; BC062370; AAH62370.1; -; mRNA.
DR CCDS; CCDS55133.1; -. [O95415-2]
DR CCDS; CCDS5656.1; -. [O95415-1]
DR RefSeq; NP_001152963.1; NM_001159491.1. [O95415-2]
DR RefSeq; NP_056194.1; NM_015379.4. [O95415-1]
DR AlphaFoldDB; O95415; -.
DR BioGRID; 117330; 5.
DR IntAct; O95415; 9.
DR STRING; 9606.ENSP00000297290; -.
DR iPTMnet; O95415; -.
DR PhosphoSitePlus; O95415; -.
DR BioMuta; BRI3; -.
DR EPD; O95415; -.
DR jPOST; O95415; -.
DR MassIVE; O95415; -.
DR MaxQB; O95415; -.
DR PaxDb; O95415; -.
DR PeptideAtlas; O95415; -.
DR PRIDE; O95415; -.
DR ProteomicsDB; 24549; -.
DR ProteomicsDB; 50863; -.
DR Antibodypedia; 49158; 51 antibodies from 19 providers.
DR DNASU; 25798; -.
DR Ensembl; ENST00000297290.4; ENSP00000297290.3; ENSG00000164713.10. [O95415-1]
DR Ensembl; ENST00000539286.5; ENSP00000440936.1; ENSG00000164713.10. [O95415-2]
DR GeneID; 25798; -.
DR KEGG; hsa:25798; -.
DR MANE-Select; ENST00000297290.4; ENSP00000297290.3; NM_015379.5; NP_056194.1.
DR UCSC; uc003upi.3; human. [O95415-1]
DR CTD; 25798; -.
DR DisGeNET; 25798; -.
DR GeneCards; BRI3; -.
DR HGNC; HGNC:1109; BRI3.
DR HPA; ENSG00000164713; Low tissue specificity.
DR MIM; 615628; gene.
DR neXtProt; NX_O95415; -.
DR OpenTargets; ENSG00000164713; -.
DR PharmGKB; PA25422; -.
DR VEuPathDB; HostDB:ENSG00000164713; -.
DR eggNOG; KOG4517; Eukaryota.
DR GeneTree; ENSGT00510000048486; -.
DR HOGENOM; CLU_138141_0_0_1; -.
DR InParanoid; O95415; -.
DR OMA; YEYGPQQ; -.
DR OrthoDB; 1433992at2759; -.
DR PhylomeDB; O95415; -.
DR TreeFam; TF329242; -.
DR PathwayCommons; O95415; -.
DR Reactome; R-HSA-6798695; Neutrophil degranulation.
DR SignaLink; O95415; -.
DR BioGRID-ORCS; 25798; 107 hits in 1073 CRISPR screens.
DR ChiTaRS; BRI3; human.
DR GenomeRNAi; 25798; -.
DR Pharos; O95415; Tbio.
DR PRO; PR:O95415; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; O95415; protein.
DR Bgee; ENSG00000164713; Expressed in right lung and 182 other tissues.
DR ExpressionAtlas; O95415; baseline and differential.
DR Genevisible; O95415; HS.
DR GO; GO:0035577; C:azurophil granule membrane; TAS:Reactome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0042802; F:identical protein binding; IDA:MGI.
DR InterPro; IPR019317; Brain_I3.
DR PANTHER; PTHR13551; PTHR13551; 1.
DR Pfam; PF10164; DUF2367; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Lysosome; Membrane; Nucleus;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..125
FT /note="Brain protein I3"
FT /id="PRO_0000064923"
FT TRANSMEM 67..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 83..98
FT /note="VGVLEDCFTFLGIFLA -> HQRSSWLYTYPLRVCT (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_060620"
FT VAR_SEQ 99..125
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_060621"
FT VARIANT 123
FT /note="T -> A (in dbSNP:rs12865)"
FT /id="VAR_033516"
FT CONFLICT 53
FT /note="H -> Y (in Ref. 6; AAH18737)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 125 AA; 13645 MW; ABBFADD00E91C52C CRC64;
MDHKPLLQER PPAYNLEAGQ GDYACGPHGY GAIPAAPPPP PYPYLVTGIP THHPRVYNIH
SRTVTRYPAN SIVVVGGCPV CRVGVLEDCF TFLGIFLAII LFPFGFICCF ALRKRRCPNC
GATFA
