THA8_ARATH
ID THA8_ARATH Reviewed; 222 AA.
AC Q9LVW6;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Protein THYLAKOID ASSEMBLY 8, chloroplastic {ECO:0000303|PubMed:22495966};
DE Short=At-THA8 {ECO:0000303|PubMed:22495966};
DE Short=AtTHA8 {ECO:0000303|PubMed:24047899};
DE AltName: Full=Protein EMBRYO DEFECTIVE 3123 {ECO:0000303|PubMed:15266054};
DE Flags: Precursor;
GN Name=THA8 {ECO:0000303|PubMed:22495966};
GN Synonyms=EMB3123 {ECO:0000303|PubMed:15266054};
GN OrderedLocusNames=At3g27750 {ECO:0000312|Araport:AT3G27750};
GN ORFNames=MGF10.16 {ECO:0000312|EMBL:BAB02699.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION [LARGE SCALE ANALYSIS], FUNCTION [LARGE SCALE ANALYSIS], AND
RP DISRUPTION PHENOTYPE [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia, and cv. No-0;
RX PubMed=15266054; DOI=10.1104/pp.104.045179;
RA Tzafrir I., Pena-Muralla R., Dickerman A., Berg M., Rogers R., Hutchens S.,
RA Sweeney T.C., McElver J., Aux G., Patton D., Meinke D.;
RT "Identification of genes required for embryo development in Arabidopsis.";
RL Plant Physiol. 135:1206-1220(2004).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=22495966; DOI=10.1261/rna.032623.112;
RA Khrouchtchova A., Monde R.-A., Barkan A.;
RT "A short PPR protein required for the splicing of specific group II introns
RT in angiosperm chloroplasts.";
RL RNA 18:1197-1209(2012).
RN [7]
RP FUNCTION.
RX PubMed=24047899; DOI=10.1074/jbc.m113.496828;
RA Ban T., Ke J., Chen R., Gu X., Tan M.H.E., Zhou X.E., Kang Y., Melcher K.,
RA Zhu J.-K., Xu H.E.;
RT "Structure of a PLS-class pentatricopeptide repeat protein provides
RT insights into mechanism of RNA recognition.";
RL J. Biol. Chem. 288:31540-31548(2013).
CC -!- FUNCTION: Essential protein required during embryogenesis
CC (PubMed:15266054, PubMed:22495966). Mediates group II organellar RNA
CC introns splicing (e.g. ycf3-2 and trnA) (PubMed:22495966). Binds weakly
CC to specific RNA (PubMed:22495966, PubMed:24047899). Promotes the
CC biogenesis of chloroplast thylakoid membranes (PubMed:22495966).
CC {ECO:0000269|PubMed:15266054, ECO:0000269|PubMed:22495966,
CC ECO:0000269|PubMed:24047899}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:22495966}; Peripheral membrane protein
CC {ECO:0000269|PubMed:22495966}; Stromal side
CC {ECO:0000269|PubMed:22495966}.
CC -!- DISRUPTION PHENOTYPE: Pigment defective embryo arrested at cotyledon
CC stage in homozygous plants, partially rescued when grown on sucrose-
CC containing medium (PubMed:15266054, PubMed:22495966). Retarded growth
CC and greening. Reduced abundance of both the excised intron and the
CC spliced product from ycf3-2 and trnA (PubMed:22495966).
CC {ECO:0000269|PubMed:15266054, ECO:0000269|PubMed:22495966}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
CC -!- WEB RESOURCE: Name=Seed defective Arabidopsis mutants;
CC URL="http://seedgenes.org/MutantList";
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DR EMBL; AB018114; BAB02699.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77360.1; -; Genomic_DNA.
DR EMBL; AY600581; AAT68380.1; -; mRNA.
DR EMBL; AY924778; AAX23853.1; -; Genomic_DNA.
DR RefSeq; NP_189412.1; NM_113691.2.
DR AlphaFoldDB; Q9LVW6; -.
DR SMR; Q9LVW6; -.
DR STRING; 3702.AT3G27750.1; -.
DR PaxDb; Q9LVW6; -.
DR PRIDE; Q9LVW6; -.
DR ProteomicsDB; 246475; -.
DR EnsemblPlants; AT3G27750.1; AT3G27750.1; AT3G27750.
DR GeneID; 822397; -.
DR Gramene; AT3G27750.1; AT3G27750.1; AT3G27750.
DR KEGG; ath:AT3G27750; -.
DR Araport; AT3G27750; -.
DR TAIR; locus:2089154; AT3G27750.
DR eggNOG; ENOG502RXN7; Eukaryota.
DR HOGENOM; CLU_081958_2_0_1; -.
DR InParanoid; Q9LVW6; -.
DR OMA; CHLALQV; -.
DR OrthoDB; 1327283at2759; -.
DR PhylomeDB; Q9LVW6; -.
DR PRO; PR:Q9LVW6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LVW6; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IDA:TAIR.
DR GO; GO:1990825; F:sequence-specific mRNA binding; IDA:UniProtKB.
DR GO; GO:0003727; F:single-stranded RNA binding; ISS:UniProtKB.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:UniProtKB.
DR GO; GO:0000373; P:Group II intron splicing; IMP:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR044190; THA8-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR47594; PTHR47594; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; mRNA processing; mRNA splicing; Plastid;
KW Reference proteome; Repeat; RNA-binding; Thylakoid; Transit peptide.
FT TRANSIT 1..32
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 33..222
FT /note="Protein THYLAKOID ASSEMBLY 8, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000442039"
FT REPEAT 115..149
FT /note="PPR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00708"
FT REPEAT 150..184
FT /note="PPR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00708"
SQ SEQUENCE 222 AA; 24849 MW; 61B833AE5B645CC9 CRC64;
MALSLSQTRP PSLSHSHTLS VIVPKRTFVS IRCGPRDNRG PLLKGRILST EAIQSIQSLK
RAHRTGVSLS LTLRPLRRLI KSDLISVLRE LLRQDYCTLA VHVLSTLRTE YPPLDLVLYA
DIVNALTRNK EFDEIDRLIG EIDGIDQRSD DKALAKLIRA VVGAERRESV VRVYTLMRES
GWGSESWEAD EYVAEVLSKG LLRLGEPDLA SQVSLKSSIL KP
