ID   THAA_BURTA              Reviewed;         238 AA.
AC   Q2T4M4;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Transcriptional repressor ThaA {ECO:0000305};
GN   Name=thaA {ECO:0000303|PubMed:20853892}; OrderedLocusNames=BTH_II1681;
OS   Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 /
OS   E264).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=271848;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=16336651; DOI=10.1186/1471-2164-6-174;
RA   Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C.,
RA   DeShazer D.;
RT   "Bacterial genome adaptation to niches: divergence of the potential
RT   virulence genes in three Burkholderia species of different survival
RT   strategies.";
RL   BMC Genomics 6:174-174(2005).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=20853892; DOI=10.1021/ja105003g;
RA   Ishida K., Lincke T., Behnken S., Hertweck C.;
RT   "Induced biosynthesis of cryptic polyketide metabolites in a Burkholderia
RT   thailandensis quorum sensing mutant.";
RL   J. Am. Chem. Soc. 132:13966-13968(2010).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX   PubMed=29914944; DOI=10.1128/aac.00463-18;
RA   Wozniak C.E., Lin Z., Schmidt E.W., Hughes K.T., Liou T.G.;
RT   "Thailandamide, a Fatty Acid Synthesis Antibiotic That Is Coexpressed with
RT   a Resistant Target Gene.";
RL   Antimicrob. Agents Chemother. 62:0-0(2018).
CC   -!- FUNCTION: Represses thailandamide production.
CC       {ECO:0000305|PubMed:20853892}.
CC   -!- DISRUPTION PHENOTYPE: No production of thailandamide antibiotic. When
CC       the promoter region is disrupted (121 bases before the start codon)
CC       thailandamide production increases (PubMed:20853892). In 2 other
CC       promoter disruption mutants (617 and 624 bases before the start codon)
CC       this bacterium no longer inhibits growth of Salmonella in an overlay
CC       assay, suggesting no thailandamide is produced (PubMed:29914944).
CC       {ECO:0000269|PubMed:20853892, ECO:0000269|PubMed:29914944}.
CC   -!- MISCELLANEOUS: Thailandamide is a polyketide that is toxic to human
CC       cell lines but also has antibacterial activity on E.coli, S.typhimurium
CC       and S.aureus. It probably acts on acetyl-CoA carboxylase in the fatty
CC       acid synthesis pathway, which is rarely found to be an antibiotic
CC       target. These data suggest it might be a good starting point for
CC       engineering of novel antibiotics. {ECO:0000305|PubMed:29914944}.
CC   -!- SIMILARITY: Belongs to the autoinducer-regulated transcriptional
CC       regulatory protein family. {ECO:0000305}.
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DR   EMBL; CP000085; ABC34138.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q2T4M4; -.
DR   SMR; Q2T4M4; -.
DR   EnsemblBacteria; ABC34138; ABC34138; BTH_II1681.
DR   KEGG; bte:BTH_II1681; -.
DR   HOGENOM; CLU_072786_7_0_4; -.
DR   OMA; NINFHIQ; -.
DR   Proteomes; UP000001930; Chromosome II.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.80; -; 2.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR   InterPro; IPR036693; TF_LuxR_autoind-bd_dom_sf.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03472; Autoind_bind; 1.
DR   Pfam; PF00196; GerE; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF75516; SSF75516; 1.
DR   PROSITE; PS00622; HTH_LUXR_1; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Transcription; Transcription regulation.
FT   CHAIN           1..238
FT                   /note="Transcriptional repressor ThaA"
FT                   /id="PRO_0000452503"
FT   DOMAIN          169..234
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        193..212
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
SQ   SEQUENCE   238 AA;  26136 MW;  CA5A9D964DBC6686 CRC64;
     MSAGREQFVR RMRASRSAGD AFDVVLQRGT ELGFQFCAYR LTAPIPITRR RTFVWSNYPN
     ACAASPVLDA DRSGRGADTD AQACGVTADA TQVFESMADG LWAETSDQGV RYGWALSVRD
     RWGAVGTLKF ARGTREIVQE ELDDIEPEMI WLAHLAHDTI GSLMRDETIP GEIARVSLVE
     RQILLWTAEG KTVSEISSIL QMSVRNINFH IQNVVGKLGA TNKTHAAVKA TLVGLIPV