ID   THAD_ARATH              Reviewed;         511 AA.
AC   Q9FI39;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Cytochrome P450 705A5;
DE            EC=1.14.-.-;
DE   AltName: Full=Thalian-diol desaturase;
DE            Short=AtTHAD;
GN   Name=CYP705A5; Synonyms=THAD; OrderedLocusNames=At5g47990;
GN   ORFNames=MDN11.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT   features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT   clones.";
RL   DNA Res. 6:183-195(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=18356490; DOI=10.1126/science.1154990;
RA   Field B., Osbourn A.E.;
RT   "Metabolic diversification -- independent assembly of operon-like gene
RT   clusters in different plants.";
RL   Science 320:543-547(2008).
CC   -!- FUNCTION: Converts thalian-diol to a desaturated thalian-diol.
CC       {ECO:0000269|PubMed:18356490}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed primarily in the root epidermis.
CC       {ECO:0000269|PubMed:18356490}.
CC   -!- DISRUPTION PHENOTYPE: Increased levels of thalian-diol in roots.
CC       {ECO:0000269|PubMed:18356490}.
CC   -!- MISCELLANEOUS: Constitutes with three contiguous genes an operon-like
CC       gene cluster that is involved in the thalianol pathway.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AB017064; BAB11063.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95603.1; -; Genomic_DNA.
DR   RefSeq; NP_199610.1; NM_124173.3.
DR   AlphaFoldDB; Q9FI39; -.
DR   SMR; Q9FI39; -.
DR   BioGRID; 20098; 4.
DR   STRING; 3702.AT5G47990.1; -.
DR   iPTMnet; Q9FI39; -.
DR   PaxDb; Q9FI39; -.
DR   PRIDE; Q9FI39; -.
DR   ProteomicsDB; 234215; -.
DR   EnsemblPlants; AT5G47990.1; AT5G47990.1; AT5G47990.
DR   GeneID; 834850; -.
DR   Gramene; AT5G47990.1; AT5G47990.1; AT5G47990.
DR   KEGG; ath:AT5G47990; -.
DR   Araport; AT5G47990; -.
DR   TAIR; locus:2152768; AT5G47990.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_0_1; -.
DR   InParanoid; Q9FI39; -.
DR   OMA; TNNTICK; -.
DR   OrthoDB; 702827at2759; -.
DR   PhylomeDB; Q9FI39; -.
DR   BioCyc; MetaCyc:AT5G47990-MON; -.
DR   PRO; PR:Q9FI39; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FI39; baseline and differential.
DR   Genevisible; Q9FI39; AT.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR   GO; GO:0080004; F:thalian-diol desaturase activity; IMP:TAIR.
DR   GO; GO:0051554; P:flavonol metabolic process; IMP:TAIR.
DR   GO; GO:0009958; P:positive gravitropism; IMP:TAIR.
DR   GO; GO:0048364; P:root development; IMP:TAIR.
DR   GO; GO:0080003; P:thalianol metabolic process; IMP:TAIR.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..511
FT                   /note="Cytochrome P450 705A5"
FT                   /id="PRO_0000366942"
FT   TRANSMEM        12..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         454
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   511 AA;  58441 MW;  95CD5E2EEEA36341 CRC64;
     MASMITVDFE NCFIFLLLCL FSRLSYDLFF RKTKDSRAGC ALPPSPPSLP IIGHLHLILF
     VPIHQSFKNI SSKYGPLLHL RFFNFPIVLV SSASTAYEIF KAQDVNVSSR PPPPIEESLI
     LGSSSFINTP YGDYSKFMKK FMVQKLLGPQ ALQRSRNIRA DELERFYKTL LDKAMKKQTV
     EIRNEAMKLT NNTICKMIMG RSCSEENGEA ETVRGLVTES IFLTKKHFLG AMFHKPLKKL
     GISLFAKELM NVSNRFDELL EKILVEHEEK LQEHHQTSDM LDMLLEAYGD ENAEYKITRD
     QIKSLFVDLF SAGTEASANT IQWTMAEIIK NPKICERLRE EIDSVVGKTR LVQETDLPNL
     PYLQAIVKEG LRLHPPGPVV RTFKETCEIK GFYIPEKTRL FVNVYAIMRD PDFWEDPEEF
     KPERFLASSR LGEEDEKRED MLKYIPFGSG RRACPGSHLA YTVVGSVIGM MVQHFDWIIK
     GEKINMKEGG TMTLTMAHPL KCTPVPRNLN T