THAG_BURTA
ID THAG_BURTA Reviewed; 5628 AA.
AC Q2T4N1;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Polyketide synthase ThaG {ECO:0000305};
GN Name=thaG {ECO:0000303|PubMed:20853892}; OrderedLocusNames=BTH_II1674;
OS Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 /
OS E264).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; pseudomallei group.
OX NCBI_TaxID=271848;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX PubMed=16336651; DOI=10.1186/1471-2164-6-174;
RA Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C.,
RA DeShazer D.;
RT "Bacterial genome adaptation to niches: divergence of the potential
RT virulence genes in three Burkholderia species of different survival
RT strategies.";
RL BMC Genomics 6:174-174(2005).
RN [2]
RP INDUCTION.
RC STRAIN=ATCC 700388 / DSM 13276 / CIP 106301 / E264;
RX PubMed=20853892; DOI=10.1021/ja105003g;
RA Ishida K., Lincke T., Behnken S., Hertweck C.;
RT "Induced biosynthesis of cryptic polyketide metabolites in a Burkholderia
RT thailandensis quorum sensing mutant.";
RL J. Am. Chem. Soc. 132:13966-13968(2010).
CC -!- FUNCTION: Involved in production of the polyketide antibiotic
CC thailandamide. {ECO:0000305|PubMed:20853892}.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00258};
CC Note=Binds 4 phosphopantetheines covalently. {ECO:0000255|PROSITE-
CC ProRule:PRU00258};
CC -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000305|PubMed:20853892}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expressed at basal levels. {ECO:0000269|PubMed:20853892}.
CC -!- MISCELLANEOUS: Thailandamide is a polyketide that is toxic to human
CC cell lines but also has antibacterial activity on E.coli, S.typhimurium
CC and S.aureus. It probably acts on acetyl-CoA carboxylase in the fatty
CC acid synthesis pathway, which is rarely found to be an antibiotic
CC target. These data suggest it might be a good starting point for
CC engineering of novel antibiotics. {ECO:0000305}.
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DR EMBL; CP000085; ABC34832.1; -; Genomic_DNA.
DR RefSeq; WP_011401356.1; NZ_CP008786.1.
DR SMR; Q2T4N1; -.
DR PRIDE; Q2T4N1; -.
DR EnsemblBacteria; ABC34832; ABC34832; BTH_II1674.
DR KEGG; bte:BTH_II1674; -.
DR HOGENOM; CLU_223515_0_0_4; -.
DR OMA; ATHERHA; -.
DR OrthoDB; 1123553at2; -.
DR Proteomes; UP000001930; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR Gene3D; 1.10.1200.10; -; 4.
DR Gene3D; 3.10.129.110; -; 2.
DR Gene3D; 3.40.47.10; -; 4.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020807; PKS_dehydratase.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR Pfam; PF16197; KAsynt_C_assoc; 4.
DR Pfam; PF00109; ketoacyl-synt; 4.
DR Pfam; PF02801; Ketoacyl-synt_C; 4.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF00550; PP-binding; 5.
DR Pfam; PF14765; PS-DH; 2.
DR SMART; SM00826; PKS_DH; 2.
DR SMART; SM00825; PKS_KS; 4.
DR SMART; SM00823; PKS_PP; 4.
DR SUPFAM; SSF47336; SSF47336; 5.
DR SUPFAM; SSF51735; SSF51735; 4.
DR SUPFAM; SSF53335; SSF53335; 1.
DR SUPFAM; SSF53901; SSF53901; 4.
DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 2.
DR PROSITE; PS50075; CARRIER; 5.
PE 2: Evidence at transcript level;
KW Antibiotic biosynthesis; Cytoplasm; Multifunctional enzyme;
KW Phosphopantetheine; Phosphoprotein; Repeat; Transferase.
FT CHAIN 1..5628
FT /note="Polyketide synthase ThaG"
FT /id="PRO_0000452505"
FT DOMAIN 1209..1286
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 2702..2780
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 2862..2935
FT /note="Carrier 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 4840..4914
FT /note="Carrier 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 5470..5544
FT /note="Carrier 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 552..613
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1156..1183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2426..2446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2817..2836
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3526..3546
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3917..3942
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4960..4992
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5573..5603
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 555..593
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2426..2440
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3921..3940
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4970..4989
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5576..5596
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1246
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 2896
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 4874
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 5504
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 5628 AA; 587364 MW; 4AB7F021112D134E CRC64;
MGIEQLNTTR AQHDDIAVIG IACRFPGASD YRQFWDNLCE RRVSIGEIPG ERWDWRAYWG
DPQQDANACN SRWGGFVDEV GAFDLGFFGM SAREVDKMDP QQRLALELAW HCFEDAGLRP
SAVAKRDVGV FVGIANLDYK EIVEAHASEI DAYYASGVAA SVASNRLSYW FDLRGPSVTV
DTACSGSLYA LHLAREALRR GECEMALAGG VSLLLTPRRY LGFARARMLS PTGAIRAFDD
AADGMVRGEG GGLVLLKPLS RALDDGDRIF GVLAGSAVNH SGRTYSLTYP DADAQARVIR
AAFDAAGVSP AQVSYVEAHG TGTPKGDPIE LEGLLRVFGA QPRDALAPRC AIGSVKANIG
HLESAAGIAG VIKTLLALHH GMLPPMPHFA ALNHRVDAAR FAAAGLAVAD ALAPWEAGAS
ADGASAARIA GVSAFGFAGT NAHVVLREAP AAARAAVAAG RADGAGVLCL SAKTDAALER
VRAGFVEWLA RDGARHAFAD ICAALVSRRE HFASRIAVAA CDVDGALAAL RARADTASSV
YRAGRVALDD DGNLVDASGP HDEQADHDGS GEHGEHGERA RAGADDLSRA RRRASRYVRG
ASPDAWGPSD GPTPHVPLPD YPFERTVCWT GPRPSRAVAG PARGPAVDEA VDEAVDEAGA
ADGDVFVLEP RWHLAPAADA RPAPARPEDA AVVVCVVADA AQRAAVERAL ARIATAPACM
FVEPDATMGE PARAVDALVR TLDGIADRAR ADVSGVDTSG AAAPISVWYC ADLARDARAP
IDVRAYDRLL TLLQALAKTR APLRRVLLAG VSESLAADAW PALVTVLAPR RPALAVTPVL
FDHGTPEALW VSALLQEARA AGGAAVRYAA GERSVAVLAR AANAASENAA RLAGGAPLRT
GGCYLLTGGL GGLGRLFAVR LMRRYGARVV ALGRSAHDAG VQASVAALCA EAGAGTLRYL
QADVCDAAAM AAVLDDIGRH EGRLNGVIHA AGCEDRASLA DKSLDDVHAV LAPKLAAATV
LDRLTAGLPL DFVCLFSSLA GIVGDFGCGA YALGNRLLMS YAQRADGRVD AHGRRRRVVA
IAWPLWRDGA MGFDDPVKRD RYLAASGQRM LDADEGFAWF ERLLASPSAA PVVLAGERAR
IVSWLGSIDG APVADASPGA ASSGAAAPNA ASDASRDTER AEATDAAAPI VAAGAPAHAH
ASAPRERDAH GSARLPALRG IAARVLGADA ALLDEQASFA DLGFDSIGLM DFARAVGEAF
GIDMSPAMLF SHVTLKRLAG HLATRLDDAP RAVAGARSGA AHASAGRACA AAGRADGARA
SRIDVRPPEA GAGERAADAR PPLTAPEIAP EIAPESASGF ASAPHPAPIP PAYEPIAIVG
MSGRFPDARD IDAFWSLLMS GRGAARRVDP HDRFAAPRDE AAAWLAPVPG IDEFDPLFFD
IAPAEAQRMD PRERLLLQHA WLALEDAGIG ARALADHALG MFVGAEAGEY GGLSREPRSI
VSDHNGVLAA RLSYFLDLTG PNLSVNTACS SGLVALHLAI RSLHARECGI ALAAGVNLML
SAELHDKMAR AGMLSQHGVC RALDRDADGF VPGEAVVVVA LKRLADALAD GDAIHGVIRA
SGVNYDGRTN GMTAPSGLAQ ARLLTDAWRT AGIDPAELGY VVAHGTGTRL GDPVELNALG
DALRASTGRR HFCAVTSNKP NVGHTLAASG LVSLVNLVEA LRRAVIPPSA HWREGNEFVA
WGDSPLRVNT VASAWPDGAR RLGGASAFGM SGTNAHVIVE AAEPARARAP HAPRGGVLFV
VSAKTGDALA QRLRDLADCL ASDAWSDGDL ADVAHTLLHG RQHFEHRSAV FARGKADAID
ALRAAADAVR NAPARRTGAL RLLDDYAAHL SARYAAWRDA PARDVDEARR LLEAIGDAYR
EGATPSALVV APAPGRTVRL PGYPFACERH WHPAPARAGG ASAGANAREP GARERAEAAA
APHVVVLTLT GSEPYLRDHL IDGQRVLPGA AHLDMARAAF ERVRAAGGRA ARFVALRDVV
WRAPLVAGDA GAEVHVELTP SGTAFAYRIL SRPADRAAAV GAALTLHSEG VVDELNEPAP
EPLDLDGWRA ACAGERVDGA ALHARFASLG IGYGPSHRGV AHVRLGEHAA LARLDLGALA
DADFDGFGLH PGIVDSAFQA SAALSAPRED AAVPFMLRAL HVYSRAARTM WAAIRVAAPA
GGGAGGVDTR PIDIDLVHDD GAICASLRGW VSARWRKLAG APPAARATAA LRGWRWRAAE
PDPRAAEAAT RVVLLAPDFG ALAAELAAHD AVRCEMLDDV SSHALPDAFA ARATQAFAAV
RRELAAKPGR PVLFQAVCRE ADSATAWGLA GLFKTAADEN PLASGHVVLV SESFDRARLA
AALLAERAWT GHLRYGGAGR EAFAIDADED DRDGREPAGG PPLRDDGVYA ISGGAGGIGR
AIARDIVART RAARVYLLGR SAHAPSDLFA TADERARIRY CRVDVADREQ VHAWIAGLKR
DGEALRGVVH AAGVVDDAFV VAKEPSRVHA VLRPKVAGAV WLDEATRDAP LDFFVAFSSL
ASAFGNAGQA DYAMANAFVD GYMTERRARV AGGVSRGASL SIQWPLWADA GMRMPAAIAS
RLAAATGLAP LPSAEGIAAW RAALARDDAN VAVLYGERAA IAAWLATSDA MPPRARAARP
MPAARVDLHA LRALAASLIG VDAHDINVDA DIDEYGLDAV ALAHLAREIG ERAGRADVGL
GFVRGERTLR AIARALDASA PGGDAEVDIG ADADADADNA PDAEACVECA GIDARATPPD
AGAPQRGAHA AAAEGSARDE AARSIAPLAG AGSPDGASAL RARVGARLSA LLADVLKVPV
ARLEPDAPFE RYGIDSLTVV ALNESLGRHV DALPKTLFFE YRTLAELTDY FVRRHAGAAW
FAPDARAEHG AAGALATLGA ATRATQTEQA EQSRVAPLAP PRRVFAAATA ATRSRAASAP
PAATADAIAV IGLAGRYPQA RDLDAFWRNL RDGRDCITEI PAERWNHGDF FDPQKGVAGK
TYSKWGGFIE GVDEFDAAFF NIAPRDAERM DPQERLFLQA SYQAIEDAGY ARASLGAGRV
GVFAGVMYSE YQLYGIEESA AGRPAALSGS AASIANRVSY HLDLHGPSMA VDTMCSSSLT
ALHLACRSLQ RGECELALAG GVNVSVHPNK YLMLADNRFV SSAGRCESFG AGGDGYVPAE
GVGVAVLKPL RAAIADGDAI HGVICATALN HGGKNNGYTV PNPAWQAAVI EAALGEAGVA
PGDVSYVEAH GTGTTLGDPI EIAGLARAFG EPGARRGAPC AIGSVKSNIG HAESAAGIAG
LTKVLLQMRH RMLVPSLHAD TLNPNIAFET TPFCVQRALE RWERPGDGER VAGVSSFGAG
GANAHVIVRE YRSDDERDGR DEPASTAPAR ARPAWIVLSA RNEDGLRARA AQLRELAAGC
EGDADLHAIA YTLQTGRDAM DERLAFEATS IADLIASLDA FARGEPGKKQ LRGNGRARRG
DAPPAGVADA RLARGEHRAA LDDWVRGASI DWRRAYGPGA PFGPAPRRMH LPVYPFARTR
HWLPAPLDAR RRAARAGGGL HPMLDANRSE FGRQRFVVEF DGREPWLADH RVDGRRVLPG
VAYLEMARAA LAASAPDMHA DGGATLEDVR WLRPCIVPDG GATLEIALER DGDGIAFSIS
QTSAHAQPAL CCTGRSPSRA ARGGGERIDV DAMRDAFRAA PAFDADACYR AFARRGVQYG
PSHRTIERVW ADGDRALARL RSARPADPRL VMWPGLLDGA LQSLIGLHGL DGDLLAAPYR
LARLDVHGAC GPAMWALARR AGDGALDLVL CDDAGEACVT MRGFASRAAA SWRTAAQAAG
APHEAVAGGD ARAAFGPAAE SPSAATSTSA ATSPAISTSA ATPAAADGDD WLLLPRWLAC
DLDPERDAGA RAVAPRSVLA IADDACVHDL SAAAFGGASV RRMPASDAAD AARLAAILGD
APRLDALVFV APGAHARSAQ ALIDAQESGA IALFRIVKAL LAHGYRDDAL TLAIVTEQAV
ALYPGEPIDP AHAALHGMAG VLGRDLPRWR VLCADVERAR AYAGAALVAQ AGPAGEGPRI
NRLGRWHRRA LARVDAPAAR ESAFRRGGVY VIVGGAGGLG RVLTEHLIRR ADARVVWVGR
RAADGRIAAD CASFESIGAP PLYLQADASD AGAMGAVRDA VKARYGAIHG VVHSALALRD
ATLATMTEAD FRRVLAAKLD TSVRLAEAFA DEPLDFMLFF SSFAAFSFPQ GQANYAAGCA
FQDAFAQHLA ARAPFAVKTV NWGFWGHAGV VATPAHRDRM ARLGIGSIEP DAAMRSLECL
LACDVGQLAL INVRRDDAIA PLMTPRRVAL HAPGAGPRAL DSVARVRPDA ERTAAARLHG
GLQRDEWRDV LLSLLDATFD ALGARRAAEA GGDALRAALG IAPRHARWFA ASLDWLRALR
EGAHAAAAMS ADDAWRAWAR LGAACDGDAG RSAQYALVDA TLRHLADIVT GRRKATEIMF
PKGSMALVEN VYRHHPVADY FNLAVAEIVA ARAADVVRER GVRILEVGAG TGGTTARVLA
VLRERGVRVD DYRFTDVSPG FLDHAAARFG AGAPFMSYGL FDVMRAPAAQ GIAPHGFDIV
VATNVLHATA DVRASLRHCR DALRAGGLLV VNEISDKSLF THLTFGLLDG WWAYEDAALR
IDGSPALDAH HWAFALRSEG YADVAFPWAD AHDLGQQIVL ADAGDVVDAA DAQAMRAREA
EPWAWATGAE SESVLEAVSE TETALTPAPT QSPAAPSDAT RTAALLRSLL GEALKVEPVS
IEGDGAFGDY GLDSIIGMGF VDAINRALDL SLDVTAVFEH NTVDALAAYV GSQLAARAPA
AAGARDVEPA SSLASSSASD FVSARLPAVD AAASSAFDAA PRARTGADAP DTSLASSASS
ISSARASSPA SPARDAASFD VAIVGASCRF PGADGLDALW RCIVDERSCV RDVGAKWLRT
RDPADAGADY RAAVLDGIDR FDAARFGISP REARRMDPQQ RLLLTEAWRA LQDAGDAARA
AAHRTGVFVA AGANEYGAGL DAADNPFSMT SMAPALMPNR ISYALDLRGP SEMTDTACSS
SLVALHRAVR SLRDGECDQA VVAAVNLLLS AEKFEGFAEL GFLSPSGRTR SFDAAGDGFV
RGEGAAALVL KPLAAARRDG DFVYACIKGT AVHHGGRGAA LTAPNAAGIR EAMSAAYRNA
GIDARTVSYL EAHGVGSPVG DAIELNAIRD AYAALSGEPA PAAPAASCRI GSVKPVFGHV
ELASGLLAVC KVLMALRHGV LPGVPGFERP NPHANLAGSP LVVARAAAPW PAPRDDANGA
AVPRRASVNS FGFGGVNAHV VLEEDVSSRH APRVFAQPPL DGARHWECPR EIAGAPAARP
GRPAPDAPHA RIEAVIRDAL AQALGVAPDA FELAVPLGEY GADSMLDLHL ATRIEETLGV
QLSVRELFAH RTLGALRDHV AERIARDGGR RAAEAARAPD AAMASDVAEA SVVSEATEAS
DASEASDASE ASEASEASEA SKAPADLAAL LERFRAGQMD LDDIVDLV
