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THAS_PIG
ID   THAS_PIG                Reviewed;         534 AA.
AC   P47787;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Thromboxane-A synthase;
DE            Short=TXA synthase;
DE            Short=TXS;
DE            EC=5.3.99.5 {ECO:0000269|PubMed:3745158};
DE   AltName: Full=Cytochrome P450 5A1;
DE   AltName: Full=Hydroperoxy icosatetraenoate dehydratase;
DE            EC=4.2.1.152;
GN   Name=TBXAS1; Synonyms=CYP5, CYP5A1;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Lung;
RX   PubMed=8144035; DOI=10.1016/0378-1119(94)90555-x;
RA   Shen R.F., Zhang L., Baek S.J., Tai H.H., Lee K.D.;
RT   "The porcine thromboxane synthase-encoding cDNA: sequence, mRNA expression
RT   and enzyme production in Sf9 insect cells.";
RL   Gene 140:261-265(1994).
RN   [2]
RP   CATALYTIC ACTIVITY, FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=3745158; DOI=10.1016/s0021-9258(18)67284-9;
RA   Shen R.F., Tai H.H.;
RT   "Immunoaffinity purification and characterization of thromboxane synthase
RT   from porcine lung.";
RL   J. Biol. Chem. 261:11592-11599(1986).
CC   -!- FUNCTION: Catalyzes the conversion of prostaglandin H2 (PGH2) to
CC       thromboxane A2 (TXA2), a potent inducer of blood vessel constriction
CC       and platelet aggregation (PubMed:3745158). Cleaves also PGH2 to 12-
CC       hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is
CC       known to act as a mediator of DNA damage. 12-HHT and malondialdehyde
CC       are formed stoichiometrically in the same amounts as TXA2.
CC       Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-
CC       (5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and
CC       15-HETE (By similarity). {ECO:0000250|UniProtKB:P24557,
CC       ECO:0000269|PubMed:3745158}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=prostaglandin H2 = thromboxane A2; Xref=Rhea:RHEA:17137,
CC         ChEBI:CHEBI:57405, ChEBI:CHEBI:57445; EC=5.3.99.5;
CC         Evidence={ECO:0000269|PubMed:3745158};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17138;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=prostaglandin H2 = (12S)-hydroxy-(5Z,8E,10E)-
CC         heptadecatrienoate + malonaldehyde; Xref=Rhea:RHEA:48644,
CC         ChEBI:CHEBI:57405, ChEBI:CHEBI:90694, ChEBI:CHEBI:566274;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a hydroperoxyeicosatetraenoate = an oxoeicosatetraenoate +
CC         H2O; Xref=Rhea:RHEA:55556, ChEBI:CHEBI:15377, ChEBI:CHEBI:59720,
CC         ChEBI:CHEBI:131859; EC=4.2.1.152;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55557;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-
CC         (5Z,8Z,11Z,13E)-eicosatetraenoate + H2O; Xref=Rhea:RHEA:48636,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:57410, ChEBI:CHEBI:57446;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:48637;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + AH2 =
CC         (5Z,8Z,11Z,13E,15S)-hydroxyeicosatetraenoate + A + H2O;
CC         Xref=Rhea:RHEA:48856, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:57409, ChEBI:CHEBI:57446;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:48857;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000250|UniProtKB:P24557};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=12 uM for prostaglandin H2 {ECO:0000269|PubMed:3745158};
CC         Vmax=0.23 umol/min/mg enzyme for prostaglandin H2 as substrate
CC         {ECO:0000269|PubMed:3745158};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P24557}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:P24557}; Multi-pass membrane protein
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in lung, kidney and thymus.
CC       {ECO:0000269|PubMed:8144035}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; L13128; AAA31127.1; -; mRNA.
DR   RefSeq; NP_999211.1; NM_214046.1.
DR   AlphaFoldDB; P47787; -.
DR   SMR; P47787; -.
DR   BioGRID; 1149247; 1.
DR   STRING; 9823.ENSSSCP00000017486; -.
DR   PaxDb; P47787; -.
DR   PeptideAtlas; P47787; -.
DR   Ensembl; ENSSSCT00070044468; ENSSSCP00070037469; ENSSSCG00070022371.
DR   GeneID; 397112; -.
DR   KEGG; ssc:397112; -.
DR   CTD; 6916; -.
DR   eggNOG; KOG0158; Eukaryota.
DR   InParanoid; P47787; -.
DR   OMA; WPHPETF; -.
DR   OrthoDB; 786853at2759; -.
DR   Reactome; R-SSC-211979; Eicosanoids.
DR   Reactome; R-SSC-2162123; Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Chromosome 18.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0036134; F:12-hydroxyheptadecatrienoic acid synthase activity; IEA:RHEA.
DR   GO; GO:0020037; F:heme binding; ISS:UniProtKB.
DR   GO; GO:0106256; F:hydroperoxy icosatetraenoate dehydratase activity; ISS:UniProtKB.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0004796; F:thromboxane-A synthase activity; IBA:GO_Central.
DR   GO; GO:0006690; P:icosanoid metabolic process; ISS:UniProtKB.
DR   GO; GO:0001516; P:prostaglandin biosynthetic process; ISS:UniProtKB.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 2.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Heme; Iron; Isomerase; Lipid biosynthesis; Lipid metabolism; Lyase;
KW   Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Prostaglandin biosynthesis; Prostaglandin metabolism; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..534
FT                   /note="Thromboxane-A synthase"
FT                   /id="PRO_0000052258"
FT   TOPO_DOM        1..10
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P24557"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        32..75
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:P24557"
FT   TRANSMEM        76..96
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        97..223
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P24557"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        245..336
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:P24557"
FT   TRANSMEM        337..357
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        358..534
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P24557"
FT   BINDING         480
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P14779"
SQ   SEQUENCE   534 AA;  60485 MW;  635347A1B3508715 CRC64;
     MEVLGFLSPE LNGPMVTMAL AVVLLALLKW YSTSAFSRLE KLGIRHPKPS PFIGNLTFFR
     QGFWESHMEL RKQYGPLSGY YLGRRMIVVI SDPDMIKQVL AEKFSNFTNR MATGLESKPV
     ADSILFLRDK RWEEVRSVLT SAFSPKKLNK LTPLISQACD LLLAHLERYA ESGDAFDIQR
     CYCCYTTDVV ASVAFGTQVN SSEEPEHPFV KHCRRFFAFS VPRLILVLIL SFPSIMVPLA
     RILPNKKRDE VNGFFNKLIR NVIALRDQQA AEERRQDFLQ MVLDLRHSAP SVGVENFDIV
     RQAFSSAKGC PADPSQPHLP RPLSKPLTVD EVVGQAFLFL IAGYEIITNT LSFVTYLLAT
     NPDCQEKLLR EVDDFSKKHP SPEHCSLQQG LPYLDMVLSE TLRMYPPAFR FTREAARDCE
     VLGQRIPAGT VLEVAVGALH HDPKHWPHPE TFDPERFTAE AQRLQQPFTY LPFGAGPRSC
     LGVQLGLLEI KLTLLHILRK FRFEACPETQ VPLQLESKSA LSPKNGVYIR IVPR
 
 
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