ID   THBI_THETS              Reviewed;          67 AA.
AC   P82354;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Theromin;
DE   AltName: Full=Thrombin inhibitor;
OS   Theromyzon tessulatum (Duck leech).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC   Hirudinea; Rhynchobdellida; Glossiphoniidae; Theromyzon.
OX   NCBI_TaxID=13286;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Head;
RX   PubMed=10837466; DOI=10.1074/jbc.m000787200;
RA   Salzet M., Chopin V., Baert J.-L., Matias I., Malecha J.;
RT   "Theromin, a novel leech thrombin inhibitor.";
RL   J. Biol. Chem. 275:30774-30780(2000).
CC   -!- FUNCTION: Potent thrombin-specific inhibitor.
CC       {ECO:0000269|PubMed:10837466}.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Eight disulfide bonds are present.
CC   -!- MASS SPECTROMETRY: Mass=14491; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:10837466};
CC   -!- SIMILARITY: Belongs to the protease inhibitor I15 (antistasin) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P82354; -.
DR   SMR; P82354; -.
DR   MEROPS; I15.003; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004094; Antistasin-like.
DR   PROSITE; PS51252; ANTISTASIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..67
FT                   /note="Theromin"
FT                   /id="PRO_0000155198"
FT   DOMAIN          2..27
FT                   /note="Antistasin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   SITE            36..37
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   67 AA;  7257 MW;  1956FEE93A57B4FA CRC64;
     ECENTECPRA CPGEYEFDED GCNTCVCKGC DDAQCRCSSD ANGCESFCTC NTRCSAADEC
     NPRCTCK