ID   THCL_BACBA              Reviewed;          14 AA.
AC   P0C8P7;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   12-AUG-2020, entry version 19.
DE   RecName: Full=Thiocillin;
OS   Bacillus badius.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1455;
RN   [1]
RP   CHARACTERIZATION, AND SUBCELLULAR LOCATION.
RC   STRAIN=AR-91;
RX   PubMed=819410; DOI=10.7164/antibiotics.29.366;
RA   Shoji J., Hinoo H., Wakisaka Y., Koizumi K., Mayama M.;
RT   "Isolation of three new antibiotics, thiocillins I, II and III, related to
RT   micrococcin P. Studies on antibiotics from the genus Bacillus. VIII.";
RL   J. Antibiot. 29:366-374(1976).
RN   [2]
RP   STRUCTURE BY NMR, DEHYDRATION AT THR-4 AND THR-13, METHYLATION AT THR-8,
RP   AND HYDROXYLATION AT VAL-6.
RC   STRAIN=AR-91;
RX   PubMed=7328054; DOI=10.7164/antibiotics.34.1126;
RA   Shoji J., Kato T., Yoshimura Y., Tori K.;
RT   "Structural studies on thiocillins I, II and III (studies on antibiotics
RT   from the genus Bacillus XXIX).";
RL   J. Antibiot. 34:1126-1136(1981).
CC   -!- FUNCTION: Has bacteriocidal activity against Gram-positive bacteria,
CC       but not against Gram-negative bacteria. Inhibits bacterial protein
CC       biosynthesis by acting on the elongation factor Tu (EF-Tu).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:819410}.
CC   -!- PTM: Maturation of thiazole and oxazole containing antibiotics involves
CC       the enzymatic condensation of a Cys, Ser or Thr with the alpha-carbonyl
CC       of the preceding amino acid to form a thioether or ether bond, then
CC       dehydration to form a double bond with the alpha-amino nitrogen.
CC       Thiazoline or oxazoline ring are dehydrogenated to form thiazole or
CC       oxazole rings.
CC   -!- PTM: Maturation of pyridinyl containing antibiotics involves the cross-
CC       linking of a Ser and a Cys-Ser pair usually separated by 7 or 8
CC       residues along the peptide chain. The Ser residues are dehydrated to
CC       didehydroalanines, then bonded between their beta carbons. The alpha
CC       carbonyl of the Cys condenses with alpha carbon of the first Ser to
CC       form a pyridinyl ring. The ring may be multiply dehydrogenated to form
CC       a pyridine ring with loss of the amino nitrogen of the first Ser.
CC   -!- PTM: 3 isomers may exist: thiocillin I, identified in B.cereus G-15,
CC       thiocillin II, identified in both B.badius and B.cereus G-15, and
CC       thiocillin III identified in B.badius only. The structural differences
CC       between them lie in the extent of the modifications at two positions,
CC       as shown in the feature table.
CC   -!- PTM: The structure of the 2,3-didehydrobutyrines is not discussed in
CC       PubMed:7328054. However, in Fig. 1 the residues are diagrammed as Z-
CC       isomers.
CC   -!- SIMILARITY: Belongs to the thiocillin family. {ECO:0000305}.
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DR   iPTMnet; P0C8P7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Hydroxylation; Methylation;
KW   Secreted; Thioether bond.
FT   PEPTIDE         1..14
FT                   /note="Thiocillin"
FT                   /id="PRO_0000363168"
FT   SITE            6
FT                   /note="Not hydroxylated; in form thiocillin III"
FT   MOD_RES         4
FT                   /note="(Z)-2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000269|PubMed:7328054"
FT   MOD_RES         6
FT                   /note="3-hydroxyvaline (Val); in form thiocillin II"
FT                   /evidence="ECO:0000269|PubMed:7328054"
FT   MOD_RES         8
FT                   /note="O-methylthreonine; in form thiocillin II and form
FT                   thiocillin III"
FT                   /evidence="ECO:0000269|PubMed:7328054"
FT   MOD_RES         13
FT                   /note="(Z)-2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000269|PubMed:7328054"
FT   MOD_RES         14
FT                   /note="Decarboxylated threonine"
FT                   /evidence="ECO:0000250"
FT   CROSSLNK        1..10
FT                   /note="Pyridine-2,5-dicarboxylic acid (Ser-Ser) (with C-9)"
FT   CROSSLNK        1..9
FT                   /note="Pyridine-2,5-dicarboxylic acid (Ser-Cys) (with S-
FT                   10)"
FT   CROSSLNK        1..2
FT                   /note="Thiazole-4-carboxylic acid (Ser-Cys)"
FT   CROSSLNK        4..5
FT                   /note="Thiazole-4-carboxylic acid (Thr-Cys)"
FT   CROSSLNK        6..7
FT                   /note="Thiazole-4-carboxylic acid (Val-Cys)"
FT   CROSSLNK        8..9
FT                   /note="Thiazole-4-carboxylic acid (Thr-Cys)"
FT   CROSSLNK        10..11
FT                   /note="Thiazole-4-carboxylic acid (Ser-Cys)"
FT   CROSSLNK        11..12
FT                   /note="Thiazole-4-carboxylic acid (Cys-Cys)"
SQ   SEQUENCE   14 AA;  1416 MW;  9C6D61FAF3777EAE CRC64;
     SCTTCVCTCS CCTT