THEG_RAT
ID THEG_RAT Reviewed; 378 AA.
AC Q5XHX8;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Testicular haploid expressed gene protein;
GN Name=Theg;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-290, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: May be involved (but not essential) in spermatogenesis.
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CCT5. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Note=Localized
CC predominantly in the nucleus of haploid round spermatid. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5XHX8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5XHX8-2; Sequence=VSP_028448;
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DR EMBL; AABR03056546; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC083921; AAH83921.1; -; mRNA.
DR RefSeq; NP_001013120.1; NM_001013102.1. [Q5XHX8-2]
DR RefSeq; XP_006240919.1; XM_006240857.2. [Q5XHX8-1]
DR AlphaFoldDB; Q5XHX8; -.
DR STRING; 10116.ENSRNOP00000010549; -.
DR iPTMnet; Q5XHX8; -.
DR PhosphoSitePlus; Q5XHX8; -.
DR PaxDb; Q5XHX8; -.
DR Ensembl; ENSRNOT00000010549; ENSRNOP00000010549; ENSRNOG00000007960. [Q5XHX8-1]
DR Ensembl; ENSRNOT00000042792; ENSRNOP00000044536; ENSRNOG00000007960. [Q5XHX8-2]
DR GeneID; 299599; -.
DR KEGG; rno:299599; -.
DR UCSC; RGD:1309240; rat. [Q5XHX8-1]
DR CTD; 51298; -.
DR RGD; 1309240; Theg.
DR eggNOG; ENOG502SBEN; Eukaryota.
DR GeneTree; ENSGT00940000154630; -.
DR HOGENOM; CLU_061711_0_0_1; -.
DR InParanoid; Q5XHX8; -.
DR OMA; YAWISPR; -.
DR OrthoDB; 1299841at2759; -.
DR PhylomeDB; Q5XHX8; -.
DR TreeFam; TF329290; -.
DR PRO; PR:Q5XHX8; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Bgee; ENSRNOG00000007960; Expressed in testis and 2 other tissues.
DR Genevisible; Q5XHX8; RN.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; ISO:RGD.
DR InterPro; IPR006623; THEG.
DR InterPro; IPR042401; THEG-like.
DR PANTHER; PTHR15901; PTHR15901; 1.
DR Pfam; PF14912; THEG; 5.
DR SMART; SM00705; THEG; 7.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Differentiation; Nucleus;
KW Phosphoprotein; Reference proteome; Repeat; Spermatogenesis.
FT CHAIN 1..378
FT /note="Testicular haploid expressed gene protein"
FT /id="PRO_0000306269"
FT REPEAT 113..132
FT /note="THEG 1"
FT REPEAT 179..198
FT /note="THEG 2"
FT REPEAT 217..236
FT /note="THEG 3"
FT REPEAT 253..272
FT /note="THEG 4"
FT REPEAT 285..304
FT /note="THEG 5"
FT REPEAT 321..340
FT /note="THEG 6"
FT REPEAT 355..374
FT /note="THEG 7"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 47..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 290
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT VAR_SEQ 300..378
FT /note="TPKALSEKCVPDRSPQWEVLNVTKNAVASSRIISLAQPKIRKDLNEGYNPYY
FT ISPASLVAQASPRIYELAVPKHITKKV -> KLKHPDVGARQTAQRVRALAALPEDRGS
FT IPPSTHTAAHNRQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028448"
SQ SEQUENCE 378 AA; 43448 MW; C53035638760B57D CRC64;
MGELGEHRSR SSLLSIPVLD TKTSGGSEYR ESDGSLDLQS TLFEDHWLQS SQATTERNTD
DPEEEIPPEE MVGEELPEVS NLEDSLRRDL EVEVVGMSHL SINERTTPTT SSAKCRKKKN
HRLLELAKPK FNWQCLKDRT GRCCKGHVWI SPRKTNLQFC LYWPSVYWTE RFIEDTTLTI
TVPEVSRRVE ELSRPKRFYQ EYYNNNRTTP IWPIPRSTLE YQASNRLKRL ATPKIRNNIW
SINMSEVSQV SRAAQMAVPT PRTLRLAKPR APATLLEEWD PMPKPKPYVS DYNRLLQLAT
PKALSEKCVP DRSPQWEVLN VTKNAVASSR IISLAQPKIR KDLNEGYNPY YISPASLVAQ
ASPRIYELAV PKHITKKV
