ID   BRK1B_BOMMX             Reviewed;         208 AA.
AC   Q90WZ1;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Kininogen-1b;
DE   Contains:
DE     RecName: Full=Maximakinin;
DE     AltName: Full=Bombinakinin M;
DE   Contains:
DE     RecName: Full=Bradykinin;
DE   Flags: Precursor;
OS   Bombina maxima (Giant fire-bellied toad) (Chinese red belly toad).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Bombinatoridae; Bombina.
OX   NCBI_TaxID=161274;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAL02326.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 41-59, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000312|EMBL:AAL02326.1};
RX   PubMed=11500030; DOI=10.1006/bbrc.2001.5359;
RA   Lai R., Liu H., Lee W.H., Zhang Y.;
RT   "A novel bradykinin-related peptide from skin secretions of toad Bombina
RT   maxima and its precursor containing six identical copies of the final
RT   product.";
RL   Biochem. Biophys. Res. Commun. 286:259-263(2001).
CC   -!- FUNCTION: In vitro, produces constriction of guinea pig ileum smooth
CC       muscle. May target bradykinin receptors (BDKRB).
CC       {ECO:0000269|PubMed:11500030}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11500030}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:11500030}.
CC   -!- MASS SPECTROMETRY: [Maximakinin]: Mass=2179; Mass_error=0.4;
CC       Method=FAB; Evidence={ECO:0000269|PubMed:11500030};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   EMBL; AY046319; AAL02326.1; -; mRNA.
DR   PIR; JC7755; JC7755.
DR   AlphaFoldDB; Q90WZ1; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; NAS:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IDA:UniProtKB.
DR   GO; GO:0045987; P:positive regulation of smooth muscle contraction; IDA:UniProtKB.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR009608; Bradykinin.
DR   Pfam; PF06753; Bradykinin; 6.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Repeat; Secreted; Signal;
KW   Toxin; Vasoactive; Vasodilator.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..208
FT                   /note="Kininogen-1b"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000003403"
FT   PEPTIDE         41..59
FT                   /note="Maximakinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003404"
FT   PEPTIDE         51..59
FT                   /note="Bradykinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003405"
FT   PEPTIDE         69..87
FT                   /note="Maximakinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003406"
FT   PEPTIDE         79..87
FT                   /note="Bradykinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003407"
FT   PEPTIDE         97..115
FT                   /note="Maximakinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003408"
FT   PEPTIDE         107..115
FT                   /note="Bradykinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003409"
FT   PEPTIDE         125..143
FT                   /note="Maximakinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003410"
FT   PEPTIDE         135..143
FT                   /note="Bradykinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003411"
FT   PEPTIDE         153..171
FT                   /note="Maximakinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003412"
FT   PEPTIDE         163..171
FT                   /note="Bradykinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003413"
FT   PEPTIDE         181..199
FT                   /note="Maximakinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003414"
FT   PEPTIDE         191..199
FT                   /note="Bradykinin"
FT                   /evidence="ECO:0000269|PubMed:11500030"
FT                   /id="PRO_0000003415"
FT   REGION          27..208
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        27..47
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   208 AA;  24104 MW;  4EA9A7773E22711D CRC64;
     MRLWFCLSFF IVLCLEHFPE TLADERNVPE SEEKTEQYLR DLPKINRKGP RPPGFSPFRG
     KFHSQSLRDL PKINRKGPRP PGFSPFRGKF HSQSLRDLPK INRKGPRPPG FSPFRGKFHS
     QSLRDLPKIN RKGPRPPGFS PFRGKFHSQS LRDLPKINRK GPRPPGFSPF RGKFHSQSLR
     DLPKINRKGP RPPGFSPFRG KFHSQSHV