BRK1_ARATH
ID BRK1_ARATH Reviewed; 85 AA.
AC Q94JY4; Q9ZQ49;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein BRICK 1;
DE Short=AtBRK1;
GN Name=BRK1; OrderedLocusNames=At2g22640; ORFNames=T9I22.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=12015123; DOI=10.1016/s0960-9822(02)00819-9;
RA Frank M.J., Smith L.G.;
RT "A small, novel protein highly conserved in plants and animals promotes the
RT polarized growth and division of maize leaf epidermal cells.";
RL Curr. Biol. 12:849-853(2002).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=15316111; DOI=10.1105/tpc.104.023739;
RA Brembu T., Winge P., Seem M., Bones A.M.;
RT "NAPP and PIRP encode subunits of a putative wave regulatory protein
RT complex involved in plant cell morphogenesis.";
RL Plant Cell 16:2335-2349(2004).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=15296760; DOI=10.1016/j.cub.2004.06.062;
RA El-Din El-Assal S., Le J., Basu D., Mallery E.L., Szymanski D.B.;
RT "Arabidopsis GNARLED encodes a NAP125 homolog that positively regulates
RT ARP2/3.";
RL Curr. Biol. 14:1405-1409(2004).
RN [8]
RP INTERACTION WITH SCAR1 AND SCAR3.
RX PubMed=15534215; DOI=10.1073/pnas.0407392101;
RA Frank M., Egile C., Dyachok J., Djakovic S., Nolasco M., Li R., Smith L.G.;
RT "Activation of Arp2/3 complex-dependent actin polymerization by plant
RT proteins distantly related to Scar/WAVE.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16379-16384(2004).
RN [9]
RP INTERACTION WITH SCAR2.
RX PubMed=16006582; DOI=10.1105/tpc.104.028670;
RA Zhang X., Dyachok J., Krishnakumar S., Smith L.G., Oppenheimer D.G.;
RT "IRREGULAR TRICHOME BRANCH1 in Arabidopsis encodes a plant homolog of the
RT actin-related protein2/3 complex activator Scar/WAVE that regulates actin
RT and microtubule organization.";
RL Plant Cell 17:2314-2326(2005).
CC -!- FUNCTION: Involved in regulation of actin and microtubule organization.
CC Part of a WAVE complex that activates the Arp2/3 complex.
CC {ECO:0000269|PubMed:12015123}.
CC -!- SUBUNIT: Binds SCAR1 and/or SCAR2 and/or SCAR3.
CC -!- INTERACTION:
CC Q94JY4; Q94JY4: BRK1; NbExp=3; IntAct=EBI-1547691, EBI-1547691;
CC Q94JY4; Q6AWX6: SCAR1; NbExp=2; IntAct=EBI-1547691, EBI-1547775;
CC Q94JY4; Q5XPJ9: SCAR2; NbExp=3; IntAct=EBI-1547691, EBI-1547795;
CC Q94JY4; Q9LP46: SCAR3; NbExp=3; IntAct=EBI-1547691, EBI-1547699;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC -!- TISSUE SPECIFICITY: Expressed in roots, root hairs, hypocotyls,
CC cotyledons, stems, leaves, trichomes, and flowers.
CC {ECO:0000269|PubMed:15296760, ECO:0000269|PubMed:15316111}.
CC -!- SIMILARITY: Belongs to the BRK1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD15572.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC006340; AAD15572.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07332.1; -; Genomic_DNA.
DR EMBL; AF370530; AAK48957.1; -; mRNA.
DR EMBL; BT000017; AAN15336.1; -; mRNA.
DR EMBL; AY085644; AAM62865.1; -; mRNA.
DR PIR; A84615; A84615.
DR RefSeq; NP_179849.2; NM_127829.3.
DR AlphaFoldDB; Q94JY4; -.
DR SMR; Q94JY4; -.
DR BioGRID; 2148; 13.
DR IntAct; Q94JY4; 12.
DR STRING; 3702.AT2G22640.1; -.
DR PaxDb; Q94JY4; -.
DR PRIDE; Q94JY4; -.
DR ProteomicsDB; 240705; -.
DR EnsemblPlants; AT2G22640.1; AT2G22640.1; AT2G22640.
DR GeneID; 816795; -.
DR Gramene; AT2G22640.1; AT2G22640.1; AT2G22640.
DR KEGG; ath:AT2G22640; -.
DR Araport; AT2G22640; -.
DR TAIR; locus:2066055; AT2G22640.
DR eggNOG; ENOG502S3PY; Eukaryota.
DR HOGENOM; CLU_175202_0_0_1; -.
DR InParanoid; Q94JY4; -.
DR OMA; MPCCCWE; -.
DR OrthoDB; 1579787at2759; -.
DR PhylomeDB; Q94JY4; -.
DR PRO; PR:Q94JY4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q94JY4; baseline and differential.
DR Genevisible; Q94JY4; AT.
DR GO; GO:0071944; C:cell periphery; IDA:TAIR.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0031209; C:SCAR complex; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IDA:UniProtKB.
DR GO; GO:0044877; F:protein-containing complex binding; IEA:InterPro.
DR GO; GO:0030041; P:actin filament polymerization; TAS:TAIR.
DR GO; GO:0045010; P:actin nucleation; TAS:TAIR.
DR GO; GO:0000902; P:cell morphogenesis; IMP:TAIR.
DR GO; GO:0048870; P:cell motility; IBA:GO_Central.
DR GO; GO:0008064; P:regulation of actin polymerization or depolymerization; IBA:GO_Central.
DR GO; GO:0010090; P:trichome morphogenesis; IMP:TAIR.
DR InterPro; IPR033378; BRICK1.
DR PANTHER; PTHR33668; PTHR33668; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..85
FT /note="Protein BRICK 1"
FT /id="PRO_0000165368"
FT COILED 45..79
FT /evidence="ECO:0000255"
SQ SEQUENCE 85 AA; 9481 MW; CEF8D18BEB4B761C CRC64;
MAKAGGITNA VNVGIAVQAD WENREFISHI SLNVRRLFEF LVQFESTTKS KLASLNEKLD
LLERRLEMLE VQVSTATANP SLFAT
