THH1_ARATH
ID THH1_ARATH Reviewed; 293 AA.
AC Q948R8; Q84WT2; Q8LFE7; Q9M9R0; Q9MA29;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Protein TOM THREE HOMOLOG 1;
GN Name=THH1; OrderedLocusNames=At1g14530; ORFNames=F14L17.31, T5E21.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11836427; DOI=10.1128/jvi.76.5.2491-2497.2002;
RA Yamanaka T., Imai T., Satoh R., Kawashima A., Takahashi M., Tomita K.,
RA Kubota K., Meshi T., Naito S., Ishikawa M.;
RT "Complete inhibition of tobamovirus multiplication by simultaneous
RT mutations in two homologous host genes.";
RL J. Virol. 76:2491-2497(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16847136; DOI=10.1099/vir.0.81942-0;
RA Fujisaki K., Ravelo G.B., Naito S., Ishikawa M.;
RT "Involvement of THH1, an Arabidopsis thaliana homologue of the TOM1 gene,
RT in tobamovirus multiplication.";
RL J. Gen. Virol. 87:2397-2401(2006).
CC -!- FUNCTION: Contributes to the intracellular multiplication of
CC tobamoviruses, probably being a membrane anchor promoting the formation
CC of the replication complex. {ECO:0000269|PubMed:16847136}.
CC -!- SUBUNIT: Constituent of tobamovirus replication complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: When associated with TOM1 disruption, reduced
CC efficiency of intracellular multiplication of tobamoviruses (e.g.
CC crucifer strain TMV-Cg), characterized by a reduced accumulation of
CC viral coat protein (CP) and reduced amplification of TMV-related RNAs.
CC {ECO:0000269|PubMed:16847136}.
CC -!- SIMILARITY: Belongs to the plant tobamovirus multiplication TOM1
CC protein family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF43955.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAF63179.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM61457.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB057678; BAB68339.1; -; mRNA.
DR EMBL; AC010657; AAF63179.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC012188; AAF43955.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE29177.1; -; Genomic_DNA.
DR EMBL; BT002800; AAO22624.1; -; mRNA.
DR EMBL; BT020325; AAV85680.1; -; mRNA.
DR EMBL; BT021134; AAX22269.1; -; mRNA.
DR EMBL; AY084894; AAM61457.1; ALT_INIT; mRNA.
DR PIR; B86280; B86280.
DR RefSeq; NP_849661.1; NM_179330.4.
DR AlphaFoldDB; Q948R8; -.
DR STRING; 3702.AT1G14530.1; -.
DR PaxDb; Q948R8; -.
DR ProteomicsDB; 234217; -.
DR EnsemblPlants; AT1G14530.2; AT1G14530.2; AT1G14530.
DR GeneID; 838015; -.
DR Gramene; AT1G14530.2; AT1G14530.2; AT1G14530.
DR KEGG; ath:AT1G14530; -.
DR Araport; AT1G14530; -.
DR TAIR; locus:2012552; AT1G14530.
DR eggNOG; ENOG502QQMF; Eukaryota.
DR HOGENOM; CLU_059685_0_0_1; -.
DR InParanoid; Q948R8; -.
DR OMA; VYIVQIT; -.
DR OrthoDB; 1067070at2759; -.
DR PRO; PR:Q948R8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q948R8; baseline and differential.
DR Genevisible; Q948R8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; ISS:UniProtKB.
DR InterPro; IPR040226; THH1/TOM1/TOM3.
DR InterPro; IPR009457; THH1/TOM1/TOM3_dom.
DR PANTHER; PTHR31142; PTHR31142; 1.
DR Pfam; PF06454; DUF1084; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix; Vacuole.
FT CHAIN 1..293
FT /note="Protein TOM THREE HOMOLOG 1"
FT /id="PRO_0000423671"
FT TOPO_DOM 1..38
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..74
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..107
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 129..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..173
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..255
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..293
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 72
FT /note="T -> A (in Ref. 4; AAO22624)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="W -> G (in Ref. 4; AAO22624)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 293 AA; 34049 MW; 22064752ADF9FD17 CRC64;
MRSGGLEMMS SSAIVAFNLK EGKNWWWDVN ESPVWQDRIF HVLAVLYGIV SVIAVIQLVR
IQLRVPEYGW TTQKVFHFLN FMVNGVRALV FLFRRDAQNM QPEILQHILL DIPSLAFFTT
YALLVLFWAE IYYQARAVST DGLRPSFFTI NAVVYVIQIA LWLVLWWKPV HLMVIISKMF
FAGVSLFAAL GFLLYGGRLF LMLQRFPVES KGRRKKLQEV GYVTTICFTC FLIRCIMMCF
DAFDDAADLD VLDHPILNFI YYLLVEILPS SLVLFILRKL PPKRGITQYH QIQ
