THI1_SCHPO
ID THI1_SCHPO Reviewed; 775 AA.
AC P36598; P40378; Q9UTJ9;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=Thiamine repressible genes regulatory protein thi1;
DE AltName: Full=Transcription factor ntf1;
GN Name=thi1; Synonyms=ntf1; ORFNames=SPAC1486.10, SPAC6G10.01;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=8088540; DOI=10.1016/0378-1119(94)90054-x;
RA Frankhauser H., Schweingruber M.E.;
RT "Thiamine-repressible genes in Schizosaccharomyces pombe are regulated by a
RT Cys6 zinc-finger motif-containing protein.";
RL Gene 147:141-144(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE OF 1-728.
RC STRAIN=972 / ATCC 24843;
RX PubMed=8163491; DOI=10.1016/s0021-9258(17)32660-1;
RA Tang C.S.L., Bueno A., Russell P.;
RT "ntf1+ encodes a 6-cysteine zinc finger-containing transcription factor
RT that regulates the nmt1 promoter in fission yeast.";
RL J. Biol. Chem. 269:11921-11926(1994).
RN [4]
RP FUNCTION.
RX PubMed=16874521; DOI=10.1007/s00294-006-0086-x;
RA McQuire T.A., Young P.G.;
RT "Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in
RT Schizosaccharomyces pombe.";
RL Curr. Genet. 50:269-279(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-208, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Transcription factor that activates the nmt1 promoter.
CC Regulation of thiamine repressible genes. Positively regulates
CC conjugation during meiosis. {ECO:0000269|PubMed:16874521}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; X77512; CAA54648.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB62420.1; -; Genomic_DNA.
DR EMBL; L25912; AAA19010.1; -; Unassigned_DNA.
DR PIR; S41962; S41962.
DR RefSeq; NP_594098.2; NM_001019522.2.
DR AlphaFoldDB; P36598; -.
DR SMR; P36598; -.
DR BioGRID; 278078; 5.
DR STRING; 4896.SPAC1486.10.1; -.
DR iPTMnet; P36598; -.
DR MaxQB; P36598; -.
DR PaxDb; P36598; -.
DR PRIDE; P36598; -.
DR EnsemblFungi; SPAC1486.10.1; SPAC1486.10.1:pep; SPAC1486.10.
DR GeneID; 2541581; -.
DR KEGG; spo:SPAC1486.10; -.
DR PomBase; SPAC1486.10; thi1.
DR VEuPathDB; FungiDB:SPAC1486.10; -.
DR eggNOG; ENOG502RZ2S; Eukaryota.
DR HOGENOM; CLU_360982_0_0_1; -.
DR InParanoid; P36598; -.
DR OMA; FRACKHC; -.
DR PhylomeDB; P36598; -.
DR PRO; PR:P36598; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IMP:PomBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISM:PomBase.
DR GO; GO:0008270; F:zinc ion binding; ISM:PomBase.
DR GO; GO:0031047; P:gene silencing by RNA; IDA:UniProtKB.
DR GO; GO:0090180; P:positive regulation of thiamine biosynthetic process; IMP:PomBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:PomBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:PomBase.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..775
FT /note="Thiamine repressible genes regulatory protein thi1"
FT /id="PRO_0000114983"
FT DNA_BIND 39..65
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 676..695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 754..775
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 208
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT CONFLICT 10
FT /note="N -> K (in Ref. 3; AAA19010)"
FT /evidence="ECO:0000305"
FT CONFLICT 325..326
FT /note="SA -> LR (in Ref. 3; AAA19010)"
FT /evidence="ECO:0000305"
FT CONFLICT 684
FT /note="S -> C (in Ref. 3; AAA19010)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 775 AA; 88060 MW; 8EE2A48072F678FB CRC64;
MNEEIGFLKN QLFADVKDLE RKKKRRVPPE QRRRVFRACK HCRQKKIKCN GGQPCISCKT
LNIECVYAQK SQNKTLSREY LEELSERQLC LEYIFSRMCP NFNLETKNLI SISKKLSENE
NLPVSKIAEV TNELDTLVRI NDQLSRNHIS GTTEEMQSSS SLIAGEVQPG ISFRDQLKVG
KLEDTLYLGP TTSEAFIERL QNELELESIS EDDLYSKRLS PSVSYSEFDE QLLLHARSLI
PSKAVVEFLI NSFFINVQTN LFVYHPHFFK CRLEIFLAME NQIDAGFLCI LLMVLAFGNQ
YTAEQQEDVS KSNFHASNIG NRLFSAALSI FPLVLLQSDV SAVQSSLLIG LYLQSTIYEK
SSFAYFGLAI KFAVALGLHK NSDDPSLTQN SKELRNRLLW SVFCIDRFVS MTTGRRPSIP
LECISIPYPV ILPDLEIPGS QSIVENMRAV INLAKLTNEI CDSLYWNPSP SFESQVNSVR
RIYARLELWK SDLHSSVVFD ESAVQHPLFR SNAHVQMIYD NAIMLTTRVI MVKKLKDKDL
TAENRRYIQL CVESATRVIN IAHLLLTHKC LSSLSFFGLH VPFASAPILL LSLHYENSQD
IQAVVTKLWQ VLEFLSSRCE FARESLNYLK SFNKQLSRRN APDINNPIAD FQNSFQNWQS
WVGDMSHGDM LSTFKLTGES SNGSNSTPNE AFQPFDQTSS LYNVPGLNKS YVSNQPSLLT
PETFLPDPVL NLEVDKQWTA PTFLSWTELL GPTNVSEQSS HTAEQTSNLT LEKNG
