THI2_YEAST
ID THI2_YEAST Reviewed; 450 AA.
AC P38141; D6VQN6;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Thiamine biosynthesis regulatory protein;
GN Name=THI2; Synonyms=PHO6; OrderedLocusNames=YBR240C; ORFNames=YBR1624;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / GRF88;
RA Nishimura H., Nosaka K., Kaneko Y., Iwashima A.;
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- FUNCTION: Positive regulator of thiamine biosynthesis.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; D32073; BAA22093.1; -; Genomic_DNA.
DR EMBL; Z36109; CAA85203.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07356.1; -; Genomic_DNA.
DR PIR; S46117; S46117.
DR RefSeq; NP_009799.3; NM_001178588.3.
DR AlphaFoldDB; P38141; -.
DR SMR; P38141; -.
DR BioGRID; 32935; 33.
DR DIP; DIP-5362N; -.
DR IntAct; P38141; 1.
DR MINT; P38141; -.
DR STRING; 4932.YBR240C; -.
DR PaxDb; P38141; -.
DR PRIDE; P38141; -.
DR EnsemblFungi; YBR240C_mRNA; YBR240C; YBR240C.
DR GeneID; 852542; -.
DR KEGG; sce:YBR240C; -.
DR SGD; S000000444; THI2.
DR VEuPathDB; FungiDB:YBR240C; -.
DR eggNOG; ENOG502QU1Y; Eukaryota.
DR HOGENOM; CLU_050082_0_0_1; -.
DR InParanoid; P38141; -.
DR OMA; CWACRLK; -.
DR BioCyc; YEAST:G3O-29171-MON; -.
DR PRO; PR:P38141; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38141; protein.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0090180; P:positive regulation of thiamine biosynthetic process; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 4: Predicted;
KW Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW Thiamine biosynthesis; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..450
FT /note="Thiamine biosynthesis regulatory protein"
FT /id="PRO_0000114984"
FT DNA_BIND 30..57
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 450 AA; 51823 MW; 1A8B1627EC66FD93 CRC64;
MVNSKRQQRS KKVASSSKVP PTKGRTFTGC WACRFKKRRC DENRPICSLC AKHGDNCSYD
IRLMWLEENI YKVRKHSLIS SLQARKSKSK PLCQKISKSR FKQMTHFRQL SPPTSDCEDS
VHEASKETTL PNDNTFTISV RRLKIYNNAV ASVFGSMTNR DYTQKRIDKK LDELLNMVEN
DISVVNLNCS KHGPYSVFRA NPAAVTSALT DQLPSPGHSM SSAEETTTAA LSSPPEDSTS
LIDIIQGKIF GILWFNCYGN MILNRQEYTT WFINKMRNSL TTEFIRFLGK IIDDPDINMA
SCLFKECIAR WSCVDWQSIA ITMLVIIHGY TCPNLTKLLR VWFLQQKLLR FSMYPLVNFI
INNTQDLDVL YHCNGLLGNA DLFEDPYQDE LTSELHVLVT ERLVNSWKDT ILQQLCSCQD
TTLSCSQLRY WQLQLKCNQQ FYKDVYAMQD
