BRK1_DICDI
ID BRK1_DICDI Reviewed; 68 AA.
AC Q54X65;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Protein BRICK1;
GN Name=brk1; Synonyms=hspc300; ORFNames=DDB_G0279175;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP IDENTIFICATION.
RX PubMed=12956949; DOI=10.1016/s0960-9822(03)00580-3;
RA Blagg S.L., Stewart M., Sambles C., Insall R.H.;
RT "PIR121 regulates pseudopod dynamics and SCAR activity in Dictyostelium.";
RL Curr. Biol. 13:1480-1487(2003).
RN [3]
RP IDENTIFICATION IN THE WAVE COMPLEX, INTERACTION WITH SCRA, AND FUNCTION.
RX PubMed=17314411; DOI=10.1091/mbc.e06-06-0518;
RA Caracino D., Jones C., Compton M., Saxe C.L. III;
RT "The N-terminus of Dictyostelium Scar interacts with Abi and HSPC300 and is
RT essential for proper regulation and function.";
RL Mol. Biol. Cell 18:1609-1620(2007).
CC -!- FUNCTION: Involved in regulation of actin and microtubule organization.
CC {ECO:0000269|PubMed:17314411}.
CC -!- SUBUNIT: Part of a Scar/WAVE complex containing brk1, scrA, abiA, pirA
CC and napA. Interacts with scrA. {ECO:0000269|PubMed:17314411}.
CC -!- INTERACTION:
CC Q54X65; Q54NF8: scrA; NbExp=3; IntAct=EBI-1808171, EBI-1808146;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BRK1 family. {ECO:0000305}.
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DR EMBL; AAFI02000029; EAL67856.1; -; Genomic_DNA.
DR RefSeq; XP_641829.1; XM_636737.1.
DR PDB; 3PP5; X-ray; 1.50 A; A=1-68.
DR PDBsum; 3PP5; -.
DR AlphaFoldDB; Q54X65; -.
DR SMR; Q54X65; -.
DR IntAct; Q54X65; 1.
DR STRING; 44689.DDB0231424; -.
DR PaxDb; Q54X65; -.
DR EnsemblProtists; EAL67856; EAL67856; DDB_G0279175.
DR GeneID; 8621907; -.
DR KEGG; ddi:DDB_G0279175; -.
DR dictyBase; DDB_G0279175; hspc300.
DR eggNOG; ENOG502S3PY; Eukaryota.
DR HOGENOM; CLU_175202_0_0_1; -.
DR InParanoid; Q54X65; -.
DR OMA; MPCCCWE; -.
DR PhylomeDB; Q54X65; -.
DR Reactome; R-DDI-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR Reactome; R-DDI-5663213; RHO GTPases Activate WASPs and WAVEs.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR Reactome; R-DDI-9013404; RAC2 GTPase cycle.
DR Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR PRO; PR:Q54X65; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0031252; C:cell leading edge; IDA:dictyBase.
DR GO; GO:0060187; C:cell pole; IDA:dictyBase.
DR GO; GO:0005911; C:cell-cell junction; IDA:dictyBase.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0032433; C:filopodium tip; IDA:dictyBase.
DR GO; GO:0031143; C:pseudopodium; IDA:dictyBase.
DR GO; GO:0031209; C:SCAR complex; IDA:dictyBase.
DR GO; GO:0042802; F:identical protein binding; IPI:dictyBase.
DR GO; GO:0060090; F:molecular adaptor activity; IDA:dictyBase.
DR GO; GO:0044877; F:protein-containing complex binding; IEA:InterPro.
DR GO; GO:0007015; P:actin filament organization; IEA:InterPro.
DR GO; GO:0048870; P:cell motility; IMP:dictyBase.
DR GO; GO:0046847; P:filopodium assembly; IMP:dictyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0031269; P:pseudopodium assembly; IMP:dictyBase.
DR GO; GO:0008064; P:regulation of actin polymerization or depolymerization; IMP:dictyBase.
DR InterPro; IPR033378; BRICK1.
DR PANTHER; PTHR33668; PTHR33668; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..68
FT /note="Protein BRICK1"
FT /id="PRO_0000331406"
FT COILED 37..62
FT /evidence="ECO:0000255"
FT HELIX 6..64
FT /evidence="ECO:0007829|PDB:3PP5"
SQ SEQUENCE 68 AA; 8198 MW; F3FF3A098EA5D721 CRC64;
MSTKTNIQKD WEQREFIEDM SINIQKIVEF LNKFELSTRN KLSDLNEKLT ILDRQVDYLE
ATFKTVQE
