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THI4_CANAL
ID   THI4_CANAL              Reviewed;         354 AA.
AC   Q5ANB7; A0A1D8PKC0;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Thiamine thiazole synthase {ECO:0000255|HAMAP-Rule:MF_03158};
DE            EC=2.4.2.60 {ECO:0000255|HAMAP-Rule:MF_03158};
DE   AltName: Full=Thiazole biosynthetic enzyme {ECO:0000255|HAMAP-Rule:MF_03158};
GN   Name=THI4 {ECO:0000255|HAMAP-Rule:MF_03158};
GN   OrderedLocusNames=CAALFM_C305130CA; ORFNames=CaO19.13407, CaO19.5986;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- FUNCTION: Involved in biosynthesis of the thiamine precursor thiazole.
CC       Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-
CC       (2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an
CC       adenylated thiazole intermediate. The reaction includes an iron-
CC       dependent sulfide transfer from a conserved cysteine residue of the
CC       protein to a thiazole intermediate. The enzyme can only undergo a
CC       single turnover, which suggests it is a suicide enzyme. May have
CC       additional roles in adaptation to various stress conditions and in DNA
CC       damage tolerance. {ECO:0000255|HAMAP-Rule:MF_03158}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-
CC         thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-
CC         carboxylate + 2 H(+) + 3 H2O + nicotinamide; Xref=Rhea:RHEA:55708,
CC         Rhea:RHEA-COMP:14264, Rhea:RHEA-COMP:14265, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:29950,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:57540, ChEBI:CHEBI:90873,
CC         ChEBI:CHEBI:139151; EC=2.4.2.60; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_03158};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_03158};
CC       Note=Binds 1 Fe cation per subunit. {ECO:0000255|HAMAP-Rule:MF_03158};
CC   -!- SUBUNIT: Homooctamer. {ECO:0000255|HAMAP-Rule:MF_03158}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03158}.
CC       Nucleus {ECO:0000255|HAMAP-Rule:MF_03158}.
CC   -!- PTM: During the catalytic reaction, a sulfide is transferred from Cys-
CC       210 to a reaction intermediate, generating a dehydroalanine residue.
CC       {ECO:0000255|HAMAP-Rule:MF_03158}.
CC   -!- SIMILARITY: Belongs to the THI4 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03158}.
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DR   EMBL; CP017625; AOW28528.1; -; Genomic_DNA.
DR   RefSeq; XP_723197.1; XM_718104.1.
DR   AlphaFoldDB; Q5ANB7; -.
DR   SMR; Q5ANB7; -.
DR   STRING; 237561.Q5ANB7; -.
DR   PRIDE; Q5ANB7; -.
DR   GeneID; 3635160; -.
DR   KEGG; cal:CAALFM_C305130CA; -.
DR   CGD; CAL0000199344; THI4.
DR   VEuPathDB; FungiDB:C3_05130C_A; -.
DR   eggNOG; KOG2960; Eukaryota.
DR   HOGENOM; CLU_053727_0_0_1; -.
DR   InParanoid; Q5ANB7; -.
DR   OMA; MWGGGMM; -.
DR   OrthoDB; 1111148at2759; -.
DR   PRO; PR:Q5ANB7; -.
DR   Proteomes; UP000000559; Chromosome 3.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-UniRule.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016763; F:pentosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0052837; P:thiazole biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.50.50.60; -; 1.
DR   HAMAP; MF_03158; THI4; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027495; Sti35.
DR   InterPro; IPR002922; Thi4_fam.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00292; TIGR00292; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Iron; Metal-binding; NAD; Nucleus; Reference proteome;
KW   Thiamine biosynthesis; Transferase.
FT   CHAIN           1..354
FT                   /note="Thiamine thiazole synthase"
FT                   /id="PRO_0000415871"
FT   BINDING         83
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
FT   BINDING         104..105
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
FT   BINDING         112
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
FT   BINDING         177
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
FT   BINDING         212
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
FT   BINDING         227
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
FT   BINDING         305
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
FT   BINDING         315..317
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
FT   MOD_RES         210
FT                   /note="2,3-didehydroalanine (Cys)"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03158"
SQ   SEQUENCE   354 AA;  38170 MW;  19288EFF22340F66 CRC64;
     MTPPTMLQTA PVESFNLNPK STQQAINLKS DAKNGKVSFA DWNEFKFAPI RESTVSRAMT
     RRYFADLDKF AESDIVIIGA GSAGLSAAYT LGKNRPDLKI AIIEASVSPG GGCWLGGQLF
     SAMVLRKPAH LFLDDMGLDY EDEGDYVVVK HAALFMSTLM SKVLQFPNIK LFNATAVEDL
     ITRKDPATNL QRIAGVVVNW AQLDHDTQSC MDPNTINCNV VLSTSGHDGP FGAFTAKRLE
     QLGRAPRDVT AGFTKPSITT SKLQEPEPIS NFQLGGMKGL DMNKAEDAIV KGTREVVPGL
     VIAGMELAEV DGSNRMGPTF GAMALSGVKA AESVLNVLEL RKQQNEACYG AYKG
 
 
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