THI5_SCHPO
ID THI5_SCHPO Reviewed; 857 AA.
AC O94278;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Thiamine repressible genes regulatory protein thi5;
DE AltName: Full=Transcription factor ntf1 5;
GN Name=thi5; ORFNames=SPBP8B7.30c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16874521; DOI=10.1007/s00294-006-0086-x;
RA McQuire T.A., Young P.G.;
RT "Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in
RT Schizosaccharomyces pombe.";
RL Curr. Genet. 50:269-279(2006).
CC -!- FUNCTION: Transcription factor that activates the nmt1 promoter.
CC Regulation of thiamine repressible genes. Negatively regulates
CC conjugation during meiosis, by inducing negative regulators which delay
CC conjugation. Involved in thi1 regulation.
CC {ECO:0000269|PubMed:16874521}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227,
CC ECO:0000269|PubMed:16874521}.
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DR EMBL; CU329671; CAA21815.1; -; Genomic_DNA.
DR PIR; T40824; T40824.
DR RefSeq; NP_596537.1; NM_001022458.2.
DR AlphaFoldDB; O94278; -.
DR SMR; O94278; -.
DR BioGRID; 277899; 16.
DR PaxDb; O94278; -.
DR EnsemblFungi; SPBP8B7.30c.1; SPBP8B7.30c.1:pep; SPBP8B7.30c.
DR GeneID; 2541388; -.
DR KEGG; spo:SPBP8B7.30c; -.
DR PomBase; SPBP8B7.30c; thi5.
DR VEuPathDB; FungiDB:SPBP8B7.30c; -.
DR HOGENOM; CLU_360982_0_0_1; -.
DR InParanoid; O94278; -.
DR OMA; FEHICPS; -.
DR PhylomeDB; O94278; -.
DR PRO; PR:O94278; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0016021; C:integral component of membrane; ISM:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IMP:PomBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISM:PomBase.
DR GO; GO:0008270; F:zinc ion binding; ISM:PomBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:PomBase.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..857
FT /note="Thiamine repressible genes regulatory protein thi5"
FT /id="PRO_0000351074"
FT DNA_BIND 38..64
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 152..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 797..819
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 857 AA; 95579 MW; 5D74EEC81CD74A73 CRC64;
MCPSDFSSRS LFLEAKEEEY KQRRRVPLDS RRRVRRACLS CRAKKIRCSG SEPCQACIAT
PSQCKYADSL KERTPSKQFI AELSQRQKCF EYLMELLCPS LPHDTRSLVK LCKHVESSLA
SGSDVRSLLI PGSIDQSVLK VAATNNKDDS SAVKSANVSF PSSSTPPSSD SNFSSIQNTD
LNTSIKFTEN ISANAIHVNQ DKVIRSANNL IINSQPILPV RSFLDALGEP IYLSPTSSTY
FLNSVLASLQ LNSSNTPESL LIDYQSKIPE DLSSSLLPLE LNTLQSTPTS VSVGSASSQG
THEYSIINLT SNHSSFSLKA LKKIAHNLLP PVSVAERYAN EFFLRYQSFF YFYPPSVFSN
RYQILADGLV NSDTLDTGFL AVALLIVVLG HFSNNNINVL PKEVQLSHID SMIQISEQLI
SSLLNRCTLS SIQAVFLLSL YHFLTGNFKC AYSYLGFAIH SAHTLGLEHG STDNSTLNEV
STEETSIRIC WSLRVLASFL YIQSGITPII NLFPNGLNSL KLPTVVPELE ARHLPSSVFH
FVAIIKYAEI SVRSLNSLYE SSSDYLMDVS NFPKLLLKVE RIYSQARNWK LNLTHQQLNG
TNDTSDLLFH SNVSLHLFYH YLIVKVSCPI MFFYLNNWYS VKPSSFTKGL NNKPSLEDVF
SNLETCYESA KAIVQLSSKL LSAGQMDKCF YLEFEILYCS AIVLFLFSVM HLGAENPLLE
SIYLLEDMGS RTIDSKVRLE KLKSAIEIFD INSATEMPIN EPLSASFESV NKENSQSGYM
AWQNWVTELS SSNIPLGHAL GNPESNNSSN SFKPSHPSQS FLVNESLDFS SNPKEPPFLP
WSEALLNMDM QLNRLGP
