ID   BRK1_PHYSA              Reviewed;          62 AA.
AC   Q800F1;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Kininogen-1;
DE   Contains:
DE     RecName: Full=[Thr6]-phyllokinin;
DE   Contains:
DE     RecName: Full=[Thr6]-bradykinin;
DE   Flags: Precursor;
OS   Phyllomedusa sauvagei (Sauvage's leaf frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Phyllomedusa.
OX   NCBI_TaxID=8395;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAD70705.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 52-62, HYDROXYLATION AT
RP   PRO-54, SULFATION AT TYR-62, AND MASS SPECTROMETRY.
RC   TISSUE=Skin {ECO:0000312|EMBL:CAD70705.1};
RX   PubMed=14612182; DOI=10.1016/j.peptides.2003.06.002;
RA   Chen T., Shaw C.;
RT   "Cloning of the (Thr6)-phyllokinin precursor from Phyllomedusa sauvagei
RT   skin confirms a non-consensus tyrosine 0-sulfation motif.";
RL   Peptides 24:1123-1130(2003).
CC   -!- FUNCTION: [[Thr6]-bradykinin]: Inhibits ACE with a Ki of 1.6 uM, and
CC       targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth
CC       muscle preparation (ileum). In vivo, induces an early hyperalgesic
CC       effects in living rats after intraplantar injection (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: [[Thr6]-phyllokinin]: Mass=1447.79; Method=MALDI;
CC       Note=Hydroxylated.; Evidence={ECO:0000269|PubMed:14612182};
CC   -!- MASS SPECTROMETRY: [[Thr6]-phyllokinin]: Mass=1431.87; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:14612182};
CC   -!- MASS SPECTROMETRY: [[Thr6]-bradykinin]: Mass=1090.63; Method=MALDI;
CC       Note=Hydroxylated.; Evidence={ECO:0000269|PubMed:14612182};
CC   -!- MASS SPECTROMETRY: [[Thr6]-bradykinin]: Mass=1074.78; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:14612182};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Bradykinin-related peptide subfamily. {ECO:0000305}.
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DR   EMBL; AJ549500; CAD70705.1; -; mRNA.
DR   AlphaFoldDB; Q800F1; -.
DR   TCDB; 1.C.52.1.14; the dermaseptin (dermaseptin) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Hydroxylation; Secreted; Signal; Sulfation; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..62
FT                   /note="Kininogen-1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000043216"
FT   PEPTIDE         52..62
FT                   /note="[Thr6]-phyllokinin"
FT                   /id="PRO_0000043217"
FT   PEPTIDE         52..60
FT                   /note="[Thr6]-bradykinin"
FT                   /id="PRO_0000043218"
FT   REGION          24..62
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        26..42
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         54
FT                   /note="4-hydroxyproline; partial"
FT                   /evidence="ECO:0000269|PubMed:14612182"
FT   MOD_RES         62
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000269|PubMed:14612182"
SQ   SEQUENCE   62 AA;  7382 MW;  6641081ACA16C16A CRC64;
     MDILKKSLFL VLFLGLVSFS ICEEEKRDTE EEENDDEIEE ESEEKKREAP ERPPGFTPFR
     IY