ID   BRK1_PITHY              Reviewed;           9 AA.
AC   P84895;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Bradykinin;
OS   Pithecopus hypochondrialis (Orange-legged leaf frog) (Phyllomedusa
OS   hypochondrialis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=317381;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND HYDROXYLATION AT PRO-3.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16797783};
RX   PubMed=16797783; DOI=10.1016/j.peptides.2006.04.020;
RA   Brand G.D., Krause F.C., Silva L.P., Leite J.R.S.A., Melo J.A.T.,
RA   Prates M.V., Pesquero J.B., Santos E.L., Nakaie C.R., Costa-Neto C.M.,
RA   Bloch C. Jr.;
RT   "Bradykinin-related peptides from Phyllomedusa hypochondrialis.";
RL   Peptides 27:2137-2146(2006).
CC   -!- FUNCTION: Produces in vitro relaxation of rat arterial smooth muscle
CC       and constriction of intestinal smooth muscle (By similarity). May
CC       target bradykinin receptors (BDKRB). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:16797783}.
CC   -!- MASS SPECTROMETRY: Mass=1060.51; Mass_error=0.1; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16797783};
CC   -!- MASS SPECTROMETRY: Mass=1076.63; Mass_error=0.1; Method=MALDI;
CC       Note=Hydroxylated.; Evidence={ECO:0000269|PubMed:16797783};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   SMR; P84895; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Hydroxylation; Secreted; Toxin;
KW   Vasoactive; Vasodilator.
FT   PEPTIDE         1..9
FT                   /note="Bradykinin"
FT                   /evidence="ECO:0000269|PubMed:16797783"
FT                   /id="PRO_0000248462"
FT   MOD_RES         3
FT                   /note="4-hydroxyproline; partial"
FT                   /evidence="ECO:0000269|PubMed:16797783"
SQ   SEQUENCE   9 AA;  1060 MW;  3393D775B9C86777 CRC64;
     RPPGFSPFR