ID   BRK1_TRICX              Reviewed;          10 AA.
AC   P0DM72;
DT   13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2013, sequence version 1.
DT   03-AUG-2022, entry version 12.
DE   RecName: Full=Nephilakinin-1;
DE   AltName: Full=Nephilakinin-I;
OS   Trichonephila clavipes (Golden silk orbweaver) (Nephila clavipes).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Araneoidea; Nephilidae; Trichonephila.
OX   NCBI_TaxID=2585209;
RN   [1]
RP   PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, SYNTHESIS, FUNCTION,
RP   BIOASSAY, TOXIC DOSE, AND AMIDATION AT ARG-10.
RX   PubMed=16202476; DOI=10.1016/j.peptides.2005.08.011;
RA   Volsi E.C., Mendes M.A., Marques M.R., dos Santos L.D., Santos K.S.,
RA   de Souza B.M., Babieri E.F., Palma M.S.;
RT   "Multiple bradykinin-related peptides from the capture web of the spider
RT   Nephila clavipes (Araneae, Tetragnatidae).";
RL   Peptides 27:690-697(2006).
CC   -!- FUNCTION: Causes constriction on isolated rat ileum preparations and
CC       relaxation on rat duodenum muscle preparations at amounts higher than
CC       bradykinin. Is a partial agonist bradykinin receptor B2 (BDKRB2). Is
CC       moderately lethal to honeybees. May be related to the predation of
CC       insects by the spider webs. {ECO:0000269|PubMed:16202476}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TOXIC DOSE: LD(50) is 98 pmoles/mg when injected into honeybees. Dose
CC       that causes muscle constriction (ED(50)) is 7 uM. Dose that causes
CC       muscle relaxation (ED(50)) is 10 uM. {ECO:0000269|PubMed:16202476}.
CC   -!- MISCELLANEOUS: Has been extracted from the spider web.
CC       {ECO:0000305|PubMed:16202476}.
CC   -!- MISCELLANEOUS: Does not target bradykinin receptor B1 (BDKRB1).
CC       {ECO:0000305|PubMed:16202476}.
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Secreted; Toxin.
FT   PEPTIDE         1..10
FT                   /note="Nephilakinin-1"
FT                   /id="PRO_0000424404"
FT   MOD_RES         10
FT                   /note="Arginine amide"
FT                   /evidence="ECO:0000269|PubMed:16202476"
SQ   SEQUENCE   10 AA;  1075 MW;  074E4C75B9C86764 CRC64;
     GPNPGFSPFR