ID   BRK2_BOMMX              Reviewed;         114 AA.
AC   P83055;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2002, sequence version 2.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Kininogen-2;
DE   AltName: Full=BMK-2;
DE   Contains:
DE     RecName: Full=Maximakinin;
DE   Contains:
DE     RecName: Full=Bradykinin;
DE   Contains:
DE     RecName: Full=Maximakinin-associated peptide;
DE   Flags: Precursor;
OS   Bombina maxima (Giant fire-bellied toad) (Chinese red belly toad).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Bombinatoridae; Bombina.
OX   NCBI_TaxID=161274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 77-95 AND 105-114,
RP   AMIDATION AT ARG-113, AND VARIANT LEU-74.
RC   TISSUE=Skin secretion;
RX   PubMed=12948837; DOI=10.1016/s0196-9781(03)00167-0;
RA   Chen T., Bjourson A.J., McClean S., Orr D.F., O'Kane E.J., Rao P., Shaw C.;
RT   "Cloning of maximakinin precursor cDNAs from Chinese toad, Bombina maxima,
RT   venom.";
RL   Peptides 24:853-861(2003).
CC   -!- FUNCTION: Potent vasodilator. Binds B1 (BDKRB1) and B2 (BDKRB2)
CC       bradykinin receptors.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   EMBL; AJ440236; CAD29346.1; -; mRNA.
DR   AlphaFoldDB; P83055; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR009608; Bradykinin.
DR   Pfam; PF06753; Bradykinin; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Repeat; Secreted; Signal; Toxin; Vasoactive; Vasodilator.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..114
FT                   /note="Kininogen-2"
FT                   /id="PRO_0000003425"
FT   PEPTIDE         77..95
FT                   /note="Maximakinin"
FT                   /id="PRO_0000003426"
FT   PEPTIDE         87..95
FT                   /note="Bradykinin"
FT                   /id="PRO_0000003427"
FT   PEPTIDE         105..113
FT                   /note="Maximakinin-associated peptide"
FT                   /id="PRO_0000003428"
FT   REGION          35..114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        59..83
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         113
FT                   /note="Arginine amide"
FT                   /evidence="ECO:0000269|PubMed:12948837"
FT   VARIANT         74
FT                   /note="F -> L"
FT                   /evidence="ECO:0000269|PubMed:12948837"
SQ   SEQUENCE   114 AA;  13361 MW;  51D4525AB970C853 CRC64;
     MRLWFCLSFF IVLCLEHFPG TLADERNNRD YTIRTRLHGH HKPSRNNRYA IKTSIHGHHI
     PRNVPESEEK TEQFLRDLPK INRKGPRPPG FSPFRGKFHS QSLRQIPGLG PLRG