ID   BRK2_BOMOR              Reviewed;         161 AA.
AC   P83059; Q90W15;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2002, sequence version 2.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Kininogen-2;
DE   AltName: Full=BOK-2;
DE   Contains:
DE     RecName: Full=[Thr6]-bradykinin;
DE   Contains:
DE     RecName: Full=Bradykinin inhibitor peptide DV-28;
DE              Short=DV-28 amide;
DE   Flags: Precursor;
OS   Bombina orientalis (Oriental fire-bellied toad).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Bombinatoridae; Bombina.
OX   NCBI_TaxID=8346 {ECO:0000305};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 87-95 AND 115-123,
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Skin, and Skin secretion;
RX   PubMed=12217414; DOI=10.1016/s0196-9781(02)00095-5;
RA   Chen T., Orr D.F., Bjourson A.J., McClean S., O'Rourke M., Hirst D.G.,
RA   Rao P., Shaw C.;
RT   "Bradykinins and their precursor cDNAs from the skin of the fire-bellied
RT   toad (Bombina orientalis).";
RL   Peptides 23:1547-1555(2002).
RN   [2]
RP   PROTEIN SEQUENCE OF 133-160, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, MASS SPECTROMETRY, DISULFIDE BOND, AND AMIDATION AT VAL-160.
RC   TISSUE=Skin secretion;
RX   PubMed=20138946; DOI=10.1016/j.peptides.2010.01.016;
RA   Wang L., Chen Y., Yang M., Zhou M., Chen T., Sui D.Y., Shaw C.;
RT   "Peptide DV-28 amide: An inhibitor of bradykinin-induced arterial smooth
RT   muscle relaxation encoded by Bombina orientalis skin kininogen-2.";
RL   Peptides 31:979-982(2010).
CC   -!- FUNCTION: [[Thr6]-bradykinin]: Inhibits ACE with a Ki of 1.6 uM, and
CC       targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth
CC       muscle preparation (ileum). In vivo, induces an early hyperalgesic
CC       effects in living rats after intraplantar injection (By similarity).
CC       {ECO:0000250}.
CC   -!- FUNCTION: [Bradykinin inhibitor peptide DV-28]: Inhibits the
CC       bradykinin-induced in vitro relaxation of rat arterial smooth muscle
CC       and constriction of intestinal smooth muscle. May target bradykinin
CC       receptors (BDKRB). {ECO:0000269|PubMed:12217414,
CC       ECO:0000269|PubMed:20138946}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12217414,
CC       ECO:0000269|PubMed:20138946, ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:12217414, ECO:0000269|PubMed:20138946}.
CC   -!- MASS SPECTROMETRY: [Bradykinin inhibitor peptide DV-28]: Mass=3198.51;
CC       Method=MALDI; Note=The measured mass is that of bradykinin inhibitor
CC       peptide DV-28.; Evidence={ECO:0000269|PubMed:20138946};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   EMBL; AJ347942; CAC70660.1; -; mRNA.
DR   AlphaFoldDB; P83059; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IDA:UniProtKB.
DR   GO; GO:0045776; P:negative regulation of blood pressure; IDA:UniProtKB.
DR   GO; GO:0045987; P:positive regulation of smooth muscle contraction; IDA:UniProtKB.
DR   InterPro; IPR009608; Bradykinin.
DR   Pfam; PF06753; Bradykinin; 2.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Direct protein sequencing; Disulfide bond;
KW   G-protein coupled receptor impairing toxin; Repeat; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..161
FT                   /note="Kininogen-2"
FT                   /id="PRO_0000003434"
FT   PEPTIDE         87..95
FT                   /note="[Thr6]-bradykinin"
FT                   /id="PRO_0000003435"
FT   PEPTIDE         115..123
FT                   /note="[Thr6]-bradykinin"
FT                   /id="PRO_0000003436"
FT   PEPTIDE         133..160
FT                   /note="Bradykinin inhibitor peptide DV-28"
FT                   /id="PRO_0000401148"
FT   MOD_RES         160
FT                   /note="Valine amide"
FT                   /evidence="ECO:0000269|PubMed:20138946"
FT   DISULFID        150..156
FT                   /evidence="ECO:0000269|PubMed:20138946"
SQ   SEQUENCE   161 AA;  19042 MW;  200A7235C3657AAC CRC64;
     MRLWFCLSFF VVLCLEHFPG TLADERNNRD YPIRTHLHGP HIPRNNRDYP IRTHLHGHHI
     PRNVPESEEK TEQFLRDLSE ISRLQRRPPG FTPFRGKFHS QSLRDLSEIS RLQRRPPGFT
     PFRGKFHSQS LRDMYEIKGF KSAHGRPRVC PPGEQCPIWV G