ID   BRK2_BOMVA              Reviewed;          96 AA.
AC   P83057; Q90W87;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2002, sequence version 2.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Kininogen-2;
DE   AltName: Full=BVK-2;
DE   Contains:
DE     RecName: Full=[Val1,Thr3,Thr6]-bradykinin;
DE   Contains:
DE     RecName: Full=Kininogen-2-associated peptide;
DE   Flags: Precursor;
OS   Bombina variegata (Yellow-bellied toad).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Bombinatoridae; Bombina.
OX   NCBI_TaxID=8348 {ECO:0000305};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-59, AND
RP   CHARACTERIZATION.
RC   TISSUE=Skin, and Skin secretion;
RX   PubMed=12230583; DOI=10.1046/j.1432-1033.2002.03174.x;
RA   Chen T., Orr D.F., Bjourson A.J., McClean S., O'Rourke M., Hirst D.G.,
RA   Rao P., Shaw C.;
RT   "Novel bradykinins and their precursor cDNAs from European yellow-bellied
RT   toad (Bombina variegata) skin.";
RL   Eur. J. Biochem. 269:4693-4700(2002).
CC   -!- FUNCTION: [Val1,Thr3,Thr6]bradykinin: produces in vitro relaxation of
CC       rat arterial smooth muscle and constriction of intestinal smooth
CC       muscle. May target bradykinin receptors (BDKRB).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   EMBL; AJ318509; CAC47938.1; -; mRNA.
DR   AlphaFoldDB; P83057; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR009608; Bradykinin.
DR   Pfam; PF06753; Bradykinin; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Cleavage on pair of basic residues;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Secreted; Signal; Toxin; Vasoactive; Vasodilator.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..96
FT                   /note="Kininogen-2"
FT                   /id="PRO_0000003440"
FT   PEPTIDE         51..59
FT                   /note="[Val1,Thr3,Thr6]-bradykinin"
FT                   /id="PRO_0000003441"
FT   PEPTIDE         76..92
FT                   /note="Kininogen-2-associated peptide"
FT                   /id="PRO_0000003442"
SQ   SEQUENCE   96 AA;  11237 MW;  B78F54DE28E03B41 CRC64;
     MILWFCLNFL IVLCLEHFPG TLAAERNVPQ SEEKTEQFLR DLSEISRLQR VPTGFTPFRG
     KFHSQSLRGL SETKKFKAPY NIHWHCKPGL LCKNFN