ID   BRK2_PHYSA              Reviewed;          11 AA.
AC   P84697; P84695; P84696;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   26-FEB-2020, entry version 29.
DE   RecName: Full=Phyllokinin;
DE   Contains:
DE     RecName: Full=Bradykinin;
OS   Phyllomedusa sauvagei (Sauvage's leaf frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Phyllomedusa.
OX   NCBI_TaxID=8395;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SULFATION AT TYR-11, AND MASS SPECTROMETRY.
RC   TISSUE=Skin {ECO:0000269|PubMed:14612182};
RX   PubMed=14612182; DOI=10.1016/j.peptides.2003.06.002;
RA   Chen T., Shaw C.;
RT   "Cloning of the (Thr6)-phyllokinin precursor from Phyllomedusa sauvagei
RT   skin confirms a non-consensus tyrosine 0-sulfation motif.";
RL   Peptides 24:1123-1130(2003).
CC   -!- FUNCTION: May produce in vitro relaxation of rat arterial smooth muscle
CC       and constriction of intestinal smooth muscle. May target bradykinin
CC       receptors (BDKRB).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: [Phyllokinin]: Mass=1417.26; Method=MALDI;
CC       Note=Sulfated.; Evidence={ECO:0000269|PubMed:14612182};
CC   -!- MASS SPECTROMETRY: [Phyllokinin]: Mass=1334.89; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:14612182};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Secreted; Sulfation; Toxin;
KW   Vasoactive; Vasodilator.
FT   PEPTIDE         1..11
FT                   /note="Phyllokinin"
FT                   /id="PRO_0000043219"
FT   PEPTIDE         1..9
FT                   /note="Bradykinin"
FT                   /id="PRO_0000043220"
FT   MOD_RES         11
FT                   /note="Sulfotyrosine; partial"
FT                   /evidence="ECO:0000269|PubMed:14612182"
SQ   SEQUENCE   11 AA;  1337 MW;  25051393D775B9C8 CRC64;
     RPPGFSPFRI Y