ID   BRK2_PITAZ              Reviewed;          61 AA.
AC   Q0VTT8; P84934;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Probradykinin-2;
DE   Contains:
DE     RecName: Full=[Val1,Hyp2,Thr6]-bradykinyl-Gln,Thr {ECO:0000303|PubMed:17120273};
DE     AltName: Full=[Val1,Hyp2,Thr6]-bradykinin-Gln,Thr {ECO:0000303|PubMed:17120273};
DE   Contains:
DE     RecName: Full=[Val1,Hyp2,Thr6]-bradykinin {ECO:0000303|PubMed:17120273};
DE   Flags: Precursor;
GN   Name=pb-H2 {ECO:0000312|EMBL:CAK55550.1};
OS   Pithecopus azureus (Orange-legged monkey tree frog) (Phyllomedusa azurea).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=2034991;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAK55550.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-61, SUBCELLULAR
RP   LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND HYDROXYLATION AT
RP   PRO-52.
RC   TISSUE=Skin, and Skin secretion {ECO:0000269|PubMed:17120273};
RX   PubMed=17120273; DOI=10.1002/rcm.2791;
RA   Thompson A.H., Bjourson A.J., Shaw C., McClean S.;
RT   "Bradykinin-related peptides from Phyllomedusa hypochondrialis azurea: Mass
RT   spectrometric structural characterisation and cloning of precursor cDNAs.";
RL   Rapid Commun. Mass Spectrom. 20:3780-3788(2006).
CC   -!- FUNCTION: May produce in vitro relaxation of rat arterial smooth muscle
CC       and constriction of intestinal smooth muscle (By similarity). May
CC       target bradykinin receptors (BDKRB). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17120273}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:17120273}.
CC   -!- MASS SPECTROMETRY: [[Val1,Hyp2,Thr6]-bradykinyl-Gln,Thr]: Mass=1261.66;
CC       Method=MALDI; Evidence={ECO:0000269|PubMed:17120273};
CC   -!- MASS SPECTROMETRY: [[Val1,Hyp2,Thr6]-bradykinin]: Mass=1032.58;
CC       Method=MALDI; Evidence={ECO:0000269|PubMed:17120273};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Bradykinin-related peptide subfamily. {ECO:0000305}.
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DR   EMBL; AM283483; CAK55550.1; -; mRNA.
DR   AlphaFoldDB; Q0VTT8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Hydroxylation; Secreted;
KW   Signal; Toxin; Vasoactive; Vasodilator.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..50
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000250594"
FT   PEPTIDE         51..61
FT                   /note="[Val1,Hyp2,Thr6]-bradykinyl-Gln,Thr"
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000250595"
FT   PEPTIDE         51..59
FT                   /note="[Val1,Hyp2,Thr6]-bradykinin"
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000343885"
FT   REGION          24..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         52
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:17120273"
SQ   SEQUENCE   61 AA;  7258 MW;  BBF9EF9919DB1CAC CRC64;
     MSFLKKSLFL VLFLGLVSFS ICEEEKRETE EEENEDEIEE QSEEKKRFEP VPPGFTPFRQ
     T