ID   BRK3_BOMVA              Reviewed;           9 AA.
AC   P83058;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   26-FEB-2020, entry version 46.
DE   RecName: Full=[Thr6]-bradykinin;
OS   Bombina variegata (Yellow-bellied toad).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Bombinatoridae; Bombina.
OX   NCBI_TaxID=8348 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Skin secretion;
RA   Chen T.B., Orr D.F., Bjourson A.J., McClean S., Rao P.F., Shaw C.;
RT   "Cloning and post-translational processing of frog skin kininogens.";
RL   Submitted (JUL-2001) to UniProtKB.
RN   [2]
RP   SKIN SECRETION COMPOSITION.
RC   TISSUE=Skin secretion;
RX   PubMed=12230583; DOI=10.1046/j.1432-1033.2002.03174.x;
RA   Chen T., Orr D.F., Bjourson A.J., McClean S., O'Rourke M., Hirst D.G.,
RA   Rao P., Shaw C.;
RT   "Novel bradykinins and their precursor cDNAs from European yellow-bellied
RT   toad (Bombina variegata) skin.";
RL   Eur. J. Biochem. 269:4693-4700(2002).
CC   -!- FUNCTION: [Thr6]-bradykinin: inhibits ACE with a Ki of 1.6 uM, and
CC       targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth
CC       muscle preparation (ileum). In vivo, induces an early hyperalgesic
CC       effects in living rats after intraplantar injection (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1, ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Secreted; Toxin.
FT   PEPTIDE         1..9
FT                   /note="[Thr6]-bradykinin"
FT                   /id="PRO_0000043520"
SQ   SEQUENCE   9 AA;  1074 MW;  3393D771A9C86777 CRC64;
     RPPGFTPFR