ID   BRK3_PITAZ              Reviewed;          11 AA.
AC   P84935;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   26-FEB-2020, entry version 28.
DE   RecName: Full=[Val1,Hyp2,Thr6]-bradykinyl-Gln,Ser {ECO:0000303|PubMed:17120273};
DE   AltName: Full=[Val1,Hyp2,Thr6]-bradykinin-Gln,Ser {ECO:0000303|PubMed:17120273};
OS   Pithecopus azureus (Orange-legged monkey tree frog) (Phyllomedusa azurea).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=2034991;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP   SPECTROMETRY, AND HYDROXYLATION AT PRO-2.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:17120273};
RX   PubMed=17120273; DOI=10.1002/rcm.2791;
RA   Thompson A.H., Bjourson A.J., Shaw C., McClean S.;
RT   "Bradykinin-related peptides from Phyllomedusa hypochondrialis azurea: Mass
RT   spectrometric structural characterisation and cloning of precursor cDNAs.";
RL   Rapid Commun. Mass Spectrom. 20:3780-3788(2006).
CC   -!- FUNCTION: May produce in vitro relaxation of rat arterial smooth muscle
CC       and constriction of intestinal smooth muscle (By similarity). May
CC       target bradykinin receptors (BDKRB). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17120273}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:17120273}.
CC   -!- MASS SPECTROMETRY: Mass=1247.75; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17120273};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR009608; Bradykinin.
DR   Pfam; PF06753; Bradykinin; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Hydroxylation; Secreted; Toxin;
KW   Vasoactive; Vasodilator.
FT   PEPTIDE         1..11
FT                   /note="[Val1,Hyp2,Thr6]-bradykinyl-Gln,Ser"
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000250426"
FT   MOD_RES         2
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:17120273"
SQ   SEQUENCE   11 AA;  1232 MW;  2BF39687D771A9C8 CRC64;
     VPPGFTPFRQ S