ID   BRK5_PITHY              Reviewed;           9 AA.
AC   P84896;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   02-DEC-2020, entry version 29.
DE   RecName: Full=[Thr6]-bradykinin;
OS   Pithecopus hypochondrialis (Orange-legged leaf frog) (Phyllomedusa
OS   hypochondrialis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=317381;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND HYDROXYLATION AT PRO-3.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16797783};
RX   PubMed=16797783; DOI=10.1016/j.peptides.2006.04.020;
RA   Brand G.D., Krause F.C., Silva L.P., Leite J.R.S.A., Melo J.A.T.,
RA   Prates M.V., Pesquero J.B., Santos E.L., Nakaie C.R., Costa-Neto C.M.,
RA   Bloch C. Jr.;
RT   "Bradykinin-related peptides from Phyllomedusa hypochondrialis.";
RL   Peptides 27:2137-2146(2006).
CC   -!- FUNCTION: [Thr6]-bradykinin: inhibits ACE with a Ki of 1.6 uM, and
CC       targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth
CC       muscle preparation (ileum). In vivo, induces an early hyperalgesic
CC       effects in living rats after intraplantar injection (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:16797783}.
CC   -!- MASS SPECTROMETRY: Mass=1074.56; Mass_error=0.1; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16797783};
CC   -!- MASS SPECTROMETRY: Mass=1090.61; Mass_error=0.1; Method=MALDI;
CC       Note=Hydroxylated.; Evidence={ECO:0000269|PubMed:16797783};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Hydroxylation; Secreted; Toxin.
FT   PEPTIDE         1..9
FT                   /note="[Thr6]-bradykinin"
FT                   /evidence="ECO:0000269|PubMed:16797783"
FT                   /id="PRO_0000248466"
FT   MOD_RES         3
FT                   /note="4-hydroxyproline; partial"
FT                   /evidence="ECO:0000269|PubMed:16797783"
SQ   SEQUENCE   9 AA;  1074 MW;  3393D771A9C86777 CRC64;
     RPPGFTPFR