BRKP1_AGACL
ID BRKP1_AGACL Reviewed; 61 AA.
AC L0PJV8;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 1.
DT 25-MAY-2022, entry version 26.
DE RecName: Full=[Thr6]-bradykinyl-Val,Asp {ECO:0000303|PubMed:24394432};
DE AltName: Full=Bradykinin-related peptide RD-11 {ECO:0000303|PubMed:24394432};
DE Contains:
DE RecName: Full=[Thr6]-bradykinin {ECO:0000303|PubMed:24394432};
DE Flags: Precursor;
OS Agalychnis callidryas (Red-eyed tree frog) (Phyllomedusa callidryas).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC Agalychnis.
OX NCBI_TaxID=197464 {ECO:0000312|EMBL:CCJ67651.1};
RN [1] {ECO:0000312|EMBL:CCJ67651.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-61, FUNCTION,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, HYDROXYLATION
RP AT PRO-53, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Skin {ECO:0000312|EMBL:CCJ67651.1}, and
RC Skin secretion {ECO:0000303|PubMed:24394432};
RX PubMed=24394432; DOI=10.1016/j.peptides.2013.12.013;
RA Jiang Y., Xi X., Ge L., Yang N., Hou X., Ma J., Ma C., Wu Y., Guo X.,
RA Li R., Zhou M., Wang L., Chen T., Shaw C.;
RT "Bradykinin-related peptides (BRPs) from skin secretions of three genera of
RT phyllomedusine leaf frogs and their comparative pharmacological effects on
RT mammalian smooth muscles.";
RL Peptides 52:122-133(2014).
CC -!- FUNCTION: [[Thr6]-bradykinin]: Induces relaxation of rat smooth muscle
CC from tail artery (EC(50)=16.8 nM) and contraction of that from ileum
CC (EC(50)=205 nM), urinary bladder (EC(50)=895 nM) and uterus
CC (EC(50)=60.3 nM). Binds to both bradykinin receptor B1 (BDKRB1) and B2
CC (BDKRB2). {ECO:0000269|PubMed:24394432}.
CC -!- FUNCTION: [Hyp3,Thr6]-bradykinin: Induces relaxation of rat smooth
CC muscle from tail artery (EC(50)=56.7 nM) and contraction of that from
CC ileum (EC(50)=588 nM), urinary bladder (EC(50)=4.6 uM) and uterus
CC (EC(50)=3.9 nM). Binds to both bradykinin receptor B1 (BDKRB1) and B2
CC (BDKRB2). In arterial smooth muscle, the effect via BDKRB1 is stronger,
CC in uterus, ileum and urinary bladder that via BDKRB2.
CC {ECO:0000269|PubMed:24394432}.
CC -!- FUNCTION: [[Thr6]-bradykinyl-Val,Asp]: Induces relaxation of rat smooth
CC muscle from tail artery (EC(50)=10.8 nM) and contraction of that from
CC ileum (EC(50)=645 nM), urinary bladder (EC(50)=1.1 uM) and uterus
CC (EC(50)=1.2 uM). Binds to both bradykinin receptor B1 (BDKRB1) and B2
CC (BDKRB2). Apart from uterus smooth muscle, the effect via B2 is
CC stronger. {ECO:0000269|PubMed:24394432}.
CC -!- FUNCTION: [Hyp3,Thr6]-bradykinyl-Val,Asp: Induces relaxation of rat
CC smooth muscle from tail artery (EC(50)=3.5 nM) and contraction of that
CC from ileum (EC(50)=223 nM), urinary bladder (EC(50)=1.5 uM) and uterus
CC (EC(50)=356 nM). Binds to both bradykinin receptor B1 (BDKRB1) and B2
CC (BDKRB2); the effects via B2 a stronger. {ECO:0000269|PubMed:24394432}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255,
CC ECO:0000269|PubMed:24394432}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000269|PubMed:24394432}.
CC -!- MASS SPECTROMETRY: [[Thr6]-bradykinyl-Val,Asp]: Mass=1303.80;
CC Method=MALDI; Note=[Hyp3,Thr6]-bradykinyl-Val,Asp.;
CC Evidence={ECO:0000269|PubMed:24394432};
CC -!- MASS SPECTROMETRY: [[Thr6]-bradykinyl-Val,Asp]: Mass=1287.65;
CC Method=MALDI; Note=[Thr6]-bradykinyl-Val,Asp.;
CC Evidence={ECO:0000269|PubMed:24394432};
CC -!- MASS SPECTROMETRY: [[Thr6]-bradykinin]: Mass=1089.55; Method=MALDI;
CC Note=[Hyp3,Thr6]-bradykinin.; Evidence={ECO:0000269|PubMed:24394432};
CC -!- MASS SPECTROMETRY: [[Thr6]-bradykinin]: Mass=1073.35; Method=MALDI;
CC Note=Thr6-bradykinin.; Evidence={ECO:0000269|PubMed:24394432};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Bradykinin-related peptide subfamily. {ECO:0000305}.
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DR EMBL; HE967330; CCJ67651.1; -; mRNA.
DR AlphaFoldDB; L0PJV8; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0031712; F:B1 bradykinin receptor binding; IDA:UniProtKB.
DR GO; GO:0031713; F:B2 bradykinin receptor binding; IDA:UniProtKB.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:1905655; P:negative regulation of artery smooth muscle contraction; IDA:UniProtKB.
DR GO; GO:1904349; P:positive regulation of small intestine smooth muscle contraction; IDA:UniProtKB.
DR GO; GO:1904320; P:positive regulation of smooth muscle contraction involved in micturition; IDA:UniProtKB.
DR GO; GO:0070474; P:positive regulation of uterine smooth muscle contraction; IDA:UniProtKB.
DR GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR Pfam; PF03032; FSAP_sig_propep; 1.
PE 1: Evidence at protein level;
KW Amphibian defense peptide; Direct protein sequencing;
KW G-protein coupled receptor impairing toxin; Hydroxylation; Secreted;
KW Signal; Toxin; Vasoactive; Vasodilator.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..50
FT /evidence="ECO:0000305|PubMed:24394432"
FT /id="PRO_0000438936"
FT PEPTIDE 51..61
FT /note="[Thr6]-bradykinyl-Val,Asp"
FT /id="PRO_0000438937"
FT PEPTIDE 51..59
FT /note="[Thr6]-bradykinin"
FT /evidence="ECO:0000269|PubMed:24394432"
FT /id="PRO_0000438938"
FT REGION 24..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..40
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 53
FT /note="4-hydroxyproline; in form [Hyp3,Thr6]-bradykinyl-
FT Val,Asp and [Hyp3,Thr6]-bradykinin"
FT /evidence="ECO:0000269|PubMed:24394432"
SQ SEQUENCE 61 AA; 7245 MW; 9649FA9D121ACE44 CRC64;
MSFLKKSLFL VLFLGLVSFS ICEEEKRETE EEENEDEMDK ESEEKRESPE RPPGFTPFRV
D
