ID   BRKP1_PHYNA             Reviewed;          61 AA.
AC   A0A088MI62;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   26-NOV-2014, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=[Val1,Thr6]-bradykinyl-Val,Asp {ECO:0000305};
DE   Flags: Precursor;
GN   Name=BKL {ECO:0000303|PubMed:26443407};
OS   Physalaemus nattereri (Cuyaba dwarf frog) (Eupemphix nattereri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Leptodactylidae; Leiuperinae;
OC   Physalaemus.
OX   NCBI_TaxID=248869 {ECO:0000312|EMBL:AIN40269.1};
RN   [1] {ECO:0000312|EMBL:AIN40269.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-61, SUBCELLULAR
RP   LOCATION, MASS SPECTROMETRY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Skin {ECO:0000312|EMBL:AIN40269.1}, and
RC   Skin secretion {ECO:0000303|PubMed:26443407};
RX   PubMed=26443407; DOI=10.1002/rcm.7313;
RA   Barbosa E.A., Iembo T., Martins G.R., Silva L.P., Prates M.V.,
RA   Andrade A.C., Bloch C. Jr.;
RT   "Skin secretion peptides: the molecular facet of the deimatic behavior of
RT   the four-eyed frog, Physalaemus nattereri (Anura, Leptodactylidae).";
RL   Rapid Commun. Mass Spectrom. 29:2061-2068(2015).
CC   -!- FUNCTION: Induces contraction of rat ileum smooth muscle (EC(50)=2.73
CC       uM) but has no activity towards rat smooth muscle from tail artery,
CC       urinary bladder or uterus. Binds to both bradykinin receptor B1
CC       (BDKRB1) and B2 (BDKRB2); the effect via BDKRB1 is stronger.
CC       {ECO:0000250|UniProtKB:P84899}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26443407}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands. Expression levels in
CC       inguinal glands and granular glands are virtually the same.
CC       {ECO:0000269|PubMed:26443407}.
CC   -!- MASS SPECTROMETRY: Mass=1231.74; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:26443407};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Bradykinin-related peptide subfamily. {ECO:0000305}.
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DR   EMBL; KJ955469; AIN40269.1; -; mRNA.
DR   AlphaFoldDB; A0A088MI62; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..48
FT                   /evidence="ECO:0000305|PubMed:26443407"
FT                   /id="PRO_0000438932"
FT   PEPTIDE         51..61
FT                   /note="[Val1,Thr6]-bradykinyl-Val,Asp"
FT                   /evidence="ECO:0000269|PubMed:26443407"
FT                   /id="PRO_5001837609"
FT   REGION          27..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   61 AA;  7016 MW;  5275B0D261E0A455 CRC64;
     MAFLKKSLFL VLFLGVVSLS FCEEEEREEH EEEKREAEAA ESAENLISKR VPPGFTPFRV
     D