ID   BRKP1_PITAZ             Reviewed;          62 AA.
AC   Q0VTT9; P84932;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Phyllokinin-1 {ECO:0000303|PubMed:17120273};
DE   Contains:
DE     RecName: Full=Asp,Pro,Glu-[Thr6,Val10]-phyllokinin {ECO:0000303|PubMed:17120273};
DE   Contains:
DE     RecName: Full=[Thr6,Val10]-phyllokinin {ECO:0000303|PubMed:17120273};
DE   Contains:
DE     RecName: Full=[Thr6]-bradykinin {ECO:0000303|PubMed:17120273};
DE   Contains:
DE     RecName: Full=Des-Arg9-[Thr6]-bradykinin;
DE              Short=Des-Arg-[Thr6]-bradykinin {ECO:0000303|PubMed:17120273};
DE   Flags: Precursor;
GN   Name=pp-H1 {ECO:0000312|EMBL:CAK55549.1};
OS   Pithecopus azureus (Orange-legged monkey tree frog) (Phyllomedusa azurea).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=2034991;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 49-62, SUBCELLULAR
RP   LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND SULFATION AT TYR-62.
RC   TISSUE=Skin, and Skin secretion {ECO:0000269|PubMed:17120273};
RX   PubMed=17120273; DOI=10.1002/rcm.2791;
RA   Thompson A.H., Bjourson A.J., Shaw C., McClean S.;
RT   "Bradykinin-related peptides from Phyllomedusa hypochondrialis azurea: Mass
RT   spectrometric structural characterisation and cloning of precursor cDNAs.";
RL   Rapid Commun. Mass Spectrom. 20:3780-3788(2006).
CC   -!- FUNCTION: [[Thr6]-bradykinin]: Inhibits ACE with a Ki of 1.6 uM, and
CC       targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth
CC       muscle preparation (ileum). In vivo, induces an early hyperalgesic
CC       effects in living rats after intraplantar injection (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17120273}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:17120273}.
CC   -!- PTM: Asp,Pro,Glu-[Thr6,Val10]-phyllokinin and [Thr6,Val10]-phyllokinin
CC       occur in sulfated and nonsulfated forms. [Thr6]-bradykinin and Des-Arg-
CC       [Thr6]-bradykinin are nonsulfated. {ECO:0000269|PubMed:17120273}.
CC   -!- MASS SPECTROMETRY: [Asp,Pro,Glu-[Thr6,Val10]-phyllokinin]:
CC       Mass=1756.86; Method=MALDI; Note=Sulfated.;
CC       Evidence={ECO:0000269|PubMed:17120273};
CC   -!- MASS SPECTROMETRY: [Asp,Pro,Glu-[Thr6,Val10]-phyllokinin]:
CC       Mass=1676.86; Method=MALDI; Note=Nonsulfated.;
CC       Evidence={ECO:0000269|PubMed:17120273};
CC   -!- MASS SPECTROMETRY: [[Thr6,Val10]-phyllokinin]: Mass=1415.73;
CC       Method=MALDI; Note=Sulfated.; Evidence={ECO:0000269|PubMed:17120273};
CC   -!- MASS SPECTROMETRY: [[Thr6,Val10]-phyllokinin]: Mass=1335.77;
CC       Method=MALDI; Note=Nonsulfated.;
CC       Evidence={ECO:0000269|PubMed:17120273};
CC   -!- MASS SPECTROMETRY: [[Thr6]-bradykinin]: Mass=1073.61; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17120273};
CC   -!- MASS SPECTROMETRY: [Des-Arg9-[Thr6]-bradykinin]: Mass=917.42;
CC       Method=MALDI; Evidence={ECO:0000269|PubMed:17120273};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Bradykinin-related peptide subfamily. {ECO:0000305}.
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DR   EMBL; AM283482; CAK55549.1; -; mRNA.
DR   AlphaFoldDB; Q0VTT9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Secreted; Signal; Sulfation; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..48
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000250596"
FT   PEPTIDE         49..62
FT                   /note="Asp,Pro,Glu-[Thr6,Val10]-phyllokinin"
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000343886"
FT   PEPTIDE         52..62
FT                   /note="[Thr6,Val10]-phyllokinin"
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000250597"
FT   PEPTIDE         52..60
FT                   /note="[Thr6]-bradykinin"
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000343887"
FT   PEPTIDE         52..59
FT                   /note="Des-Arg9-[Thr6]-bradykinin"
FT                   /evidence="ECO:0000269|PubMed:17120273"
FT                   /id="PRO_0000343888"
FT   REGION          22..62
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        26..44
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         62
FT                   /note="Sulfotyrosine; partial"
FT                   /evidence="ECO:0000269|PubMed:17120273"
SQ   SEQUENCE   62 AA;  7386 MW;  794399AED8A48E72 CRC64;
     MSFLKKSLFL VLFLGLVSSS ICEEEKRETE EEENEDEIEE ESEEKKREDP ERPPGFTPFR
     VY