ID   BRKP1_PITHY             Reviewed;          62 AA.
AC   P84900; Q0VZ35;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   20-FEB-2007, sequence version 2.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Phyllokininogen-1;
DE   Contains:
DE     RecName: Full=Phyllokinin;
DE   Flags: Precursor;
OS   Pithecopus hypochondrialis (Orange-legged leaf frog) (Phyllomedusa
OS   hypochondrialis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=317381;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Skin;
RX   PubMed=16713656; DOI=10.1016/j.peptides.2006.04.006;
RA   Chen T., Zhou M., Gagliardo R., Walker B., Shaw C.;
RT   "Elements of the granular gland peptidome and transcriptome persist in air-
RT   dried skin of the South American orange-legged leaf frog, Phyllomedusa
RT   hypocondrialis.";
RL   Peptides 27:2129-2136(2006).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 52-62, MASS SPECTROMETRY, HYDROXYLATION AT PRO-54, AND
RP   SULFATION AT TYR-62.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16797783};
RX   PubMed=16797783; DOI=10.1016/j.peptides.2006.04.020;
RA   Brand G.D., Krause F.C., Silva L.P., Leite J.R.S.A., Melo J.A.T.,
RA   Prates M.V., Pesquero J.B., Santos E.L., Nakaie C.R., Costa-Neto C.M.,
RA   Bloch C. Jr.;
RT   "Bradykinin-related peptides from Phyllomedusa hypochondrialis.";
RL   Peptides 27:2137-2146(2006).
RN   [3]
RP   HYDROXYLATION AT PRO-54, SULFATION AT TYR-62, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RX   PubMed=17120273; DOI=10.1002/rcm.2791;
RA   Thompson A.H., Bjourson A.J., Shaw C., McClean S.;
RT   "Bradykinin-related peptides from Phyllomedusa hypochondrialis azurea: Mass
RT   spectrometric structural characterisation and cloning of precursor cDNAs.";
RL   Rapid Commun. Mass Spectrom. 20:3780-3788(2006).
CC   -!- FUNCTION: Produces in vitro relaxation of rat arterial smooth muscle
CC       and constriction of intestinal smooth muscle (By similarity). May
CC       target bradykinin receptors (BDKRB). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:16797783}.
CC   -!- MASS SPECTROMETRY: [Phyllokinin]: Mass=1336.74; Mass_error=0.1;
CC       Method=MALDI; Evidence={ECO:0000269|PubMed:16797783};
CC   -!- MASS SPECTROMETRY: [Phyllokinin]: Mass=1352.74; Mass_error=0.1;
CC       Method=MALDI; Note=Hydroxylated.;
CC       Evidence={ECO:0000269|PubMed:16797783};
CC   -!- MASS SPECTROMETRY: [Phyllokinin]: Mass=1416.70; Mass_error=0.1;
CC       Method=MALDI; Note=Sulfated.; Evidence={ECO:0000269|PubMed:16797783};
CC   -!- MASS SPECTROMETRY: [Phyllokinin]: Mass=1432.70; Mass_error=0.1;
CC       Method=MALDI; Note=Hydroxylated and sulfated.;
CC       Evidence={ECO:0000269|PubMed:16797783};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Bradykinin-related peptide subfamily. {ECO:0000305}.
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DR   EMBL; AM229017; CAJ76141.1; -; mRNA.
DR   AlphaFoldDB; P84900; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Hydroxylation; Secreted;
KW   Signal; Sulfation; Toxin; Vasoactive; Vasodilator.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..62
FT                   /note="Phyllokininogen-1"
FT                   /id="PRO_0000278793"
FT   PEPTIDE         52..62
FT                   /note="Phyllokinin"
FT                   /id="PRO_0000248470"
FT   REGION          25..62
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        26..42
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         54
FT                   /note="4-hydroxyproline; partial"
FT                   /evidence="ECO:0000269|PubMed:16797783,
FT                   ECO:0000269|PubMed:17120273"
FT   MOD_RES         62
FT                   /note="Sulfotyrosine; partial"
FT                   /evidence="ECO:0000269|PubMed:16797783,
FT                   ECO:0000269|PubMed:17120273"
SQ   SEQUENCE   62 AA;  7326 MW;  794CBF6B39C20B7D CRC64;
     MSILKKSLFL VLFLGLVSFS ICEEEKREAE EEENEDEIEE ESDEKKRESP DRPPGFSPFR
     IY