BRKP2_PHYNA
ID BRKP2_PHYNA Reviewed; 134 AA.
AC A0A088MIT0;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 26-NOV-2014, sequence version 1.
DT 25-MAY-2022, entry version 21.
DE RecName: Full=Bradykinin-related peptides {ECO:0000303|PubMed:26443407};
DE Contains:
DE RecName: Full=Nattererimorphin {ECO:0000303|PubMed:26443407};
DE Contains:
DE RecName: Full=[Val1,Thr6]-bradykinyl-Ser,Pro,Ala {ECO:0000303|PubMed:26443407};
DE Contains:
DE RecName: Full=Bradykinin {ECO:0000303|PubMed:26443407};
DE Contains:
DE RecName: Full=Des-Arg9-bradykinin {ECO:0000303|PubMed:26443407};
DE Contains:
DE RecName: Full=[Hyp3]-bradykinyl-Val,Asp {ECO:0000303|PubMed:26443407};
DE Flags: Precursor;
GN Name=BBN {ECO:0000303|PubMed:26443407};
OS Physalaemus nattereri (Cuyaba dwarf frog) (Eupemphix nattereri).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Hyloidea; Leptodactylidae; Leiuperinae;
OC Physalaemus.
OX NCBI_TaxID=248869 {ECO:0000312|EMBL:AIN40268.1};
RN [1] {ECO:0000312|EMBL:AIN40268.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 47-56; 59-70; 85-96 AND
RP 123-134, SUBCELLULAR LOCATION, MASS SPECTROMETRY, HYDROXYLATION AT PRO-126,
RP AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Skin {ECO:0000312|EMBL:AIN40268.1};
RX PubMed=26443407; DOI=10.1002/rcm.7313;
RA Barbosa E.A., Iembo T., Martins G.R., Silva L.P., Prates M.V.,
RA Andrade A.C., Bloch C. Jr.;
RT "Skin secretion peptides: the molecular facet of the deimatic behavior of
RT the four-eyed frog, Physalaemus nattereri (Anura, Leptodactylidae).";
RL Rapid Commun. Mass Spectrom. 29:2061-2068(2015).
CC -!- FUNCTION: [[Val1,Thr6]-bradykinyl-Ser,Pro,Ala]: May produce in vitro
CC relaxation of rat arterial smooth muscle and constriction of intestinal
CC smooth muscle. May target bradykinin receptors (BDKRB).
CC {ECO:0000250|UniProtKB:P84899}.
CC -!- FUNCTION: [Bradykinin]: May produce in vitro relaxation of rat arterial
CC smooth muscle and constriction of intestinal smooth muscle. May target
CC bradykinin receptors (BDKRB). {ECO:0000250|UniProtKB:L0PIN3}.
CC -!- FUNCTION: [Des-Arg9-bradykinin]: May produce in vitro relaxation of rat
CC arterial smooth muscle and constriction of intestinal smooth muscle.
CC May target bradykinin receptors (BDKRB).
CC {ECO:0000250|UniProtKB:L0PIN3}.
CC -!- FUNCTION: [[Hyp3]-bradykinyl-Val,Asp]: May produce in vitro relaxation
CC of rat arterial smooth muscle and constriction of intestinal smooth
CC muscle. May target bradykinin receptors (BDKRB).
CC {ECO:0000250|UniProtKB:L0PIN3}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26443407}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands. Expression levels in
CC inguinal glands are much higher than in granular glands.
CC {ECO:0000305|PubMed:26443407}.
CC -!- MASS SPECTROMETRY: [Nattererimorphin]: Mass=1062.59; Method=MALDI;
CC Note=Nattererimorphin.; Evidence={ECO:0000269|PubMed:26443407};
CC -!- MASS SPECTROMETRY: [[Val1,Thr6]-bradykinyl-Ser,Pro,Ala]: Mass=1272.76;
CC Method=MALDI; Note=[Val1,Thr6]-bradykinyl-Ser,Pro,Ala.;
CC Evidence={ECO:0000269|PubMed:26443407};
CC -!- MASS SPECTROMETRY: [[Hyp3]-bradykinyl-Val,Asp]: Mass=1060.65;
CC Method=MALDI; Note=Bradykinin.; Evidence={ECO:0000269|PubMed:26443407};
CC -!- MASS SPECTROMETRY: [Bradykinin]: Mass=1076.65; Method=MALDI;
CC Note=[Hyp3]-bradykinin.; Evidence={ECO:0000269|PubMed:26443407};
CC -!- MASS SPECTROMETRY: [Des-Arg9-bradykinin]: Mass=904.50; Method=MALDI;
CC Note=Des-Arg9-bradykinin.; Evidence={ECO:0000269|PubMed:26443407};
CC -!- MASS SPECTROMETRY: [[Hyp3]-bradykinyl-Val,Asp]: Mass=1290.74;
CC Method=MALDI; Note=[Hyp3]-bradykinyl-Val,Asp.;
CC Evidence={ECO:0000269|PubMed:26443407};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Bradykinin-related peptide subfamily. {ECO:0000305}.
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DR EMBL; KJ955468; AIN40268.1; -; mRNA.
DR AlphaFoldDB; A0A088MIT0; -.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR Pfam; PF03032; FSAP_sig_propep; 1.
PE 1: Evidence at protein level;
KW Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW G-protein coupled receptor impairing toxin; Hydroxylation; Secreted;
KW Signal; Toxin.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..44
FT /evidence="ECO:0000305|PubMed:26443407"
FT /id="PRO_0000438948"
FT PEPTIDE 47..56
FT /note="Nattererimorphin"
FT /evidence="ECO:0000269|PubMed:26443407"
FT /id="PRO_0000438949"
FT PEPTIDE 59..70
FT /note="[Val1,Thr6]-bradykinyl-Ser,Pro,Ala"
FT /evidence="ECO:0000269|PubMed:26443407"
FT /id="PRO_0000438950"
FT PROPEP 71..82
FT /evidence="ECO:0000305|PubMed:26443407"
FT /id="PRO_0000438951"
FT PEPTIDE 85..96
FT /note="[Val1,Thr6]-bradykinyl-Ser,Pro,Ala"
FT /evidence="ECO:0000269|PubMed:26443407"
FT /id="PRO_0000438952"
FT PROPEP 99..121
FT /evidence="ECO:0000305|PubMed:26443407"
FT /id="PRO_0000438953"
FT PEPTIDE 124..134
FT /note="[Hyp3]-bradykinyl-Val,Asp"
FT /evidence="ECO:0000269|PubMed:26443407"
FT /id="PRO_0000438956"
FT PEPTIDE 124..132
FT /note="Bradykinin"
FT /evidence="ECO:0000269|PubMed:26443407"
FT /id="PRO_0000438954"
FT PEPTIDE 124..131
FT /note="Des-Arg9-bradykinin"
FT /evidence="ECO:0000269|PubMed:26443407"
FT /id="PRO_0000438955"
FT REGION 24..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..44
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 126
FT /note="4-hydroxyproline; partial; in form [Hyp3]-bradykinin
FT and [Hyp3]-bradykinin-Val,Asp"
FT /evidence="ECO:0000269|PubMed:26443407"
SQ SEQUENCE 134 AA; 15058 MW; D162C87255B9D4E3 CRC64;
MAFLKKSLFL VLFLGVVSLS FCEEEKREEH EEEKRDEEDA ESLGKRYGGL SPLRISKRVP
PGFTPFRSPA RSISGLTPIR LSKRVPPGFT PFRSPARRIS EADPGFTPSF VVIKGLSPLR
GKRRPPGFSP FRVD
