ID   BRKP3_PHYNA             Reviewed;          11 AA.
AC   C0HKA7;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2017, sequence version 1.
DT   26-FEB-2020, entry version 8.
DE   RecName: Full=[Thr6]-phyllokinin {ECO:0000303|PubMed:26443407};
OS   Physalaemus nattereri (Cuyaba dwarf frog) (Eupemphix nattereri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Leptodactylidae; Leiuperinae;
OC   Physalaemus.
OX   NCBI_TaxID=248869 {ECO:0000303|PubMed:26443407};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, HYDROXYLATION AT
RP   PRO-3, SULFATION AT TYR-11, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000303|PubMed:26443407};
RX   PubMed=26443407; DOI=10.1002/rcm.7313;
RA   Barbosa E.A., Iembo T., Martins G.R., Silva L.P., Prates M.V.,
RA   Andrade A.C., Bloch C. Jr.;
RT   "Skin secretion peptides: the molecular facet of the deimatic behavior of
RT   the four-eyed frog, Physalaemus nattereri (Anura, Leptodactylidae).";
RL   Rapid Commun. Mass Spectrom. 29:2061-2068(2015).
CC   -!- FUNCTION: May produce in vitro relaxation of rat arterial smooth muscle
CC       and constriction of intestinal smooth muscle. May target bradykinin
CC       receptors (BDKRB). {ECO:0000250|UniProtKB:L0PIN3}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26443407}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:26443407}.
CC   -!- MASS SPECTROMETRY: Mass=1350.85; Method=MALDI; Note=[Thr6]-
CC       Phyllokinin.; Evidence={ECO:0000269|PubMed:26443407};
CC   -!- MASS SPECTROMETRY: Mass=1366.82; Method=MALDI; Note=[Hyp3,Thr6]-
CC       Phyllokinin.; Evidence={ECO:0000269|PubMed:26443407};
CC   -!- MASS SPECTROMETRY: Mass=1430.83; Method=MALDI; Note=[Thr6]-Phyllokinin,
CC       sulfated.; Evidence={ECO:0000269|PubMed:26443407};
CC   -!- MASS SPECTROMETRY: Mass=1446.78; Method=MALDI; Note=[Hyp3,Thr6]-
CC       Phyllokinin, sulfated.; Evidence={ECO:0000269|PubMed:26443407};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Hydroxylation; Secreted; Sulfation; Toxin.
FT   PEPTIDE         1..11
FT                   /note="[Thr6]-phyllokinin"
FT                   /evidence="ECO:0000269|PubMed:26443407"
FT                   /id="PRO_0000438957"
FT   MOD_RES         3
FT                   /note="4-hydroxyproline; in form [Hyp3,Thr6]-Phyllokinin"
FT                   /evidence="ECO:0000269|PubMed:26443407"
FT   MOD_RES         11
FT                   /note="Sulfotyrosine; partial; in form [Thr6]-Phyllokinin
FT                   and [Hyp3,Thr6]-Phyllokinin"
FT                   /evidence="ECO:0000269|PubMed:26443407"
SQ   SEQUENCE   11 AA;  1351 MW;  25051393D771A9C8 CRC64;
     RPPGFTPFRI Y