ID   BRKP4_PHYNA             Reviewed;           9 AA.
AC   C0HKA8;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2017, sequence version 1.
DT   26-FEB-2020, entry version 8.
DE   RecName: Full=[Thr6]-bradykinin {ECO:0000303|PubMed:26443407};
DE   Contains:
DE     RecName: Full=Des-Arg9-[Thr6]-bradykinin {ECO:0000303|PubMed:26443407};
OS   Physalaemus nattereri (Cuyaba dwarf frog) (Eupemphix nattereri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Leptodactylidae; Leiuperinae;
OC   Physalaemus.
OX   NCBI_TaxID=248869 {ECO:0000303|PubMed:26443407};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, HYDROXYLATION AT
RP   PRO-3, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000303|PubMed:26443407};
RX   PubMed=26443407; DOI=10.1002/rcm.7313;
RA   Barbosa E.A., Iembo T., Martins G.R., Silva L.P., Prates M.V.,
RA   Andrade A.C., Bloch C. Jr.;
RT   "Skin secretion peptides: the molecular facet of the deimatic behavior of
RT   the four-eyed frog, Physalaemus nattereri (Anura, Leptodactylidae).";
RL   Rapid Commun. Mass Spectrom. 29:2061-2068(2015).
CC   -!- FUNCTION: Thr6-bradykinin: Induces relaxation of rat smooth muscle from
CC       tail artery and contraction of that from ileum, urinary bladder and
CC       uterus. Binds to both bradykinin receptor B1 (BDKRB1) and B2 (BDKRB2).
CC       {ECO:0000250|UniProtKB:L0PIN3}.
CC   -!- FUNCTION: [Hyp3,Thr6]-bradykinin: May produce in vitro relaxation of
CC       rat arterial smooth muscle and constriction of intestinal smooth
CC       muscle. May target bradykinin receptors (BDKRB).
CC       {ECO:0000250|UniProtKB:L0PIN3}.
CC   -!- FUNCTION: [Des-Arg9-[Thr6]-bradykinin]: May produce in vitro relaxation
CC       of rat arterial smooth muscle and constriction of intestinal smooth
CC       muscle. May target bradykinin receptors (BDKRB).
CC       {ECO:0000250|UniProtKB:L0PIN3}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26443407}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:26443407}.
CC   -!- MASS SPECTROMETRY: [[Thr6]-bradykinin]: Mass=1074.67; Method=MALDI;
CC       Note=[Thr6]-bradykinin.; Evidence={ECO:0000269|PubMed:26443407};
CC   -!- MASS SPECTROMETRY: [[Thr6]-bradykinin]: Mass=1090.66; Method=MALDI;
CC       Note=[Hyp3,Thr6]-bradykinin.; Evidence={ECO:0000269|PubMed:26443407};
CC   -!- MASS SPECTROMETRY: [Des-Arg9-[Thr6]-bradykinin]: Mass=918.54;
CC       Method=MALDI; Note=[Thr6,des-Arg9]-bradykinin.;
CC       Evidence={ECO:0000269|PubMed:26443407};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Bradykinin receptor impairing toxin;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Hydroxylation; Secreted; Toxin.
FT   PEPTIDE         1..9
FT                   /note="[Thr6]-bradykinin"
FT                   /evidence="ECO:0000269|PubMed:26443407"
FT                   /id="PRO_0000438958"
FT   PEPTIDE         1..8
FT                   /note="Des-Arg9-[Thr6]-bradykinin"
FT                   /evidence="ECO:0000269|PubMed:26443407"
FT                   /id="PRO_0000438959"
FT   MOD_RES         3
FT                   /note="4-hydroxyproline; partial; in form [Thr6]-
FT                   bradykinin"
FT                   /evidence="ECO:0000269|PubMed:26443407"
SQ   SEQUENCE   9 AA;  1074 MW;  3393D771A9C86777 CRC64;
     RPPGFTPFR