ID   BRK_MEGFL               Reviewed;          11 AA.
AC   P12797;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   23-FEB-2022, entry version 69.
DE   RecName: Full=Megascoliakinin {ECO:0000303|PubMed:27096870};
DE            Short=MBK {ECO:0000303|PubMed:27096870};
DE   AltName: Full=Thr6-bradykinin-Lys-Ala {ECO:0000303|PubMed:3617088};
DE   Contains:
DE     RecName: Full=Thr6-bradykinin {ECO:0000303|PubMed:3617088};
DE     AltName: Full=Bradykinin-like peptide {ECO:0000303|PubMed:3617088};
DE     AltName: Full=Bradykinin-related peptide {ECO:0000305};
OS   Megascolia flavifrons (Garden dagger wasp) (Solitary wasp).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Scolioidea;
OC   Scoliidae; Megascolia.
OX   NCBI_TaxID=7437;
RN   [1]
RP   PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=3617088; DOI=10.1016/0041-0101(87)90288-1;
RA   Yasuhara T., Mantel P., Nakajima T., Piek T.;
RT   "Two kinins isolated from an extract of the venom reservoirs of the
RT   solitary wasp Megascolia flavifrons.";
RL   Toxicon 25:527-535(1987).
RN   [2]
RP   REVIEW.
RX   PubMed=27096870; DOI=10.3390/toxins8040114;
RA   Konno K., Kazuma K., Nihei K.;
RT   "Peptide toxins in solitary wasp venoms.";
RL   Toxins 8:114-114(2016).
CC   -!- FUNCTION: [Thr6-bradykinin]: Inhibits ACE with a Ki of 1.6 uM, and
CC       targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth
CC       muscle preparation (ileum). In vivo, induces an early hyperalgesic
CC       effects in living rats after intraplantar injection (By similarity).
CC       {ECO:0000250}.
CC   -!- FUNCTION: [Megascoliakinin]: Has bradykinin-like, although lower
CC       activities (e.g. smooth muscle contraction). May target bradykinin
CC       receptors (BDKRB).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3617088}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:3617088}.
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   PIR; A26744; A26744.
DR   PIR; B26744; B26744.
DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0045776; P:negative regulation of blood pressure; ISS:UniProtKB.
DR   GO; GO:0045987; P:positive regulation of smooth muscle contraction; TAS:UniProtKB.
PE   1: Evidence at protein level;
KW   Bradykinin receptor impairing toxin; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Secreted; Toxin.
FT   PEPTIDE         1..11
FT                   /note="Megascoliakinin"
FT                   /evidence="ECO:0000269|PubMed:3617088"
FT                   /id="PRO_0000003381"
FT   PEPTIDE         1..9
FT                   /note="Thr6-bradykinin"
FT                   /evidence="ECO:0000269|PubMed:3617088"
FT                   /id="PRO_0000003382"
SQ   SEQUENCE   11 AA;  1274 MW;  33867393D771A9C8 CRC64;
     RPPGFTPFRK A